Characterization and mapping of a highly conserved processed pseudogene and an intron-carrying gene of the heat shock cognate protein 70 (Hsc70) gene family in the rat

Rothermel, E; Detzler, E.; Walter, Lutz; Levan, Göran; Günther, Eberhard

doi:10.1007/bf00352365

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…Extending the R1.6 fragment by genome walking Pseudogenes that represent inactive versions of currently active genes were reported in many gene families that were interspersed in the vicinity of the clustered active genes, including hsc70 (Heschl and Baillie 1989;Rothermel et al 1995), hsp47 (Nagai et al 1999), hsp27 (Hickey et al 1986) and hsp25 (Frohli et al 1993). In order to examine whether there were any active rice class-I sHSP genes adjacent to the R1.6 fragment, we carried out PCR-based genomic walking with a LTIR-specific primer (IRSP).…”

Section: Resultsmentioning

confidence: 99%

Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Guan

Zhang

et al. 2003

Theor Appl Genet

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Small heat-shock proteins (sHSP) are the most abundant heat stress-induced proteins in plants. In rice, there are at least seven members of class-I sHSP. A 1.6-kb DNA fragment was isolated from the EcoRI-digested rice genomic library probed with the cDNA pTS1 encoding a 16.9-kDa class-I sHSP. This fragment was composed of 365-bp tandem direct repeats (DRs) and 441-bp near perfect long terminal inverted repeats (LTIRs). The DRs contain 123-bp regions with 99% nucleotide identity to the 5' coding region of the Oshsp16.9B gene. Two putative pseudogenes were deduced from the DRs. Using the LTIR as a specific probe, Southern-blotting analysis showed that there was a single copy of this 1.6-kb DNA fragment in the rice genome. By genomic walking, we located this fragment in proximity 5'-upstream of the Oshsp16.9B gene that was mapped on chromosome 1 with other two class-I sHSP genes, Oshsp16.9A and Oshsp16.9C. By comparative analysis of the nucleotide sequences of class-I sHSP genes clustered on chromosome 1 between Tainung No. 67 and Nipponbare cultivars, we confirmed our mapping results of these genes and only the promoter region of Oshsp16.9B was different. However, we found that the expression profile of Oshsp16.9B upon different heat stresses in Nipponbare was not significantly different relative to that in Tainung No. 67.

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Section: Resultsmentioning

confidence: 99%

Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Guan

Zhang

et al. 2003

Theor Appl Genet

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“…In many cancer types, high level CD44 expression is associated with unfavorable clinical outcome [28]. Heat shock proteins belong to a group of highly conserved proteins with different cellular localizations [29]. Some of these proteins have been discussed recently as biomarkers for hepatocellular carcinomas [19].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics of rat liver and Morris hepatoma 7777 plasma membranes

Clifton

Reutter

et al. 2007

Journal of Chromatography B

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“…Specific hybridization probes were derived from the 3Ј untranslated region of the human HSP70-1, HSP70-2, and HSP70-HOM genes (positions 2143-2301, 2225-2407, 2902-3242, respectively) [Milner and Campbell, 1990] and of the corresponding rat genes HSP70-1, HSP70-2, and HSP70-3 (positions 2143-2301, 1927-2075, 1992-2182 Rothermel et al, 1994] by genomic polymerase chain reaction (PCR) amplification and cloning of the resulting gene fragments into pCRII (Invitrogen, Heidelberg, Germany) or pSPT18 (Boehringer, Mannheim, Germany). Rat HSC70 transcripts were specifically detected by a 508-bp SstI fragment subcloned from rat cosmid pKE (positions 1383-1890) [Rothermel et al, 1995]. For the sake of simplicity, also rat genes will be written in capital contrary to official nomenclature.…”

Section: Gene Probesmentioning

confidence: 99%

Heat-induced expression of MHC-linked HSP70 genes in lymphocytes varies at the single-cell level

Ralf

Günther

1999

J. Cell. Biochem.

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The expression of MHC-linked heat shock protein 70 (HSP70) genes HSP70-1 and HSP70-2 has been studied in human and rat lymphocytes and Concanavalin A (con A)-induced lymphoblasts by in situ hybridization and flow cytometry. In in vitro experiments transcripts of these genes were observed only after heat shock, and mitogen stimulation per se did not lead to induction. Heat shock-induced expression of HSP70-1 and HSP70-2 mRNA varied at the single-cell level. The fraction of HSP70-positive lymphocytes increased continuously with the severity of heat shock. However, 15-35% of the cells always remained HSP70 transcript negative. After fever induction in vivo similar variation of HSP70-1 and HSP70-2 mRNA expression could also be observed. Analysis of heat shocked lymphocytes by flow cytometry demonstrated that HSP70 induction varied also at the protein level, a fraction of cells always remaining unresponsive. Thus, HSP70 induction does not appear to occur in each cell and to a similar extent when a given cell population is exposed to the same stress.

show abstract

Characterization and mapping of a highly conserved processed pseudogene and an intron-carrying gene of the heat shock cognate protein 70 (Hsc70) gene family in the rat

Cited by 9 publications

References 19 publications

Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Comparative proteomics of rat liver and Morris hepatoma 7777 plasma membranes

Heat-induced expression of MHC-linked HSP70 genes in lymphocytes varies at the single-cell level

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