“…Specific hybridization probes were derived from the 3Ј untranslated region of the human HSP70-1, HSP70-2, and HSP70-HOM genes (positions 2143-2301, 2225-2407, 2902-3242, respectively) [Milner and Campbell, 1990] and of the corresponding rat genes HSP70-1, HSP70-2, and HSP70-3 (positions 2143-2301, 1927-2075, 1992-2182 Rothermel et al, 1994] by genomic polymerase chain reaction (PCR) amplification and cloning of the resulting gene fragments into pCRII (Invitrogen, Heidelberg, Germany) or pSPT18 (Boehringer, Mannheim, Germany). Rat HSC70 transcripts were specifically detected by a 508-bp SstI fragment subcloned from rat cosmid pKE (positions 1383-1890) [Rothermel et al, 1995]. For the sake of simplicity, also rat genes will be written in capital contrary to official nomenclature.…”