Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Guan, Jiahn Chou; Li, X.-H.; Zhang, Q.-F.; Kochert, Gary; Lin, Ching Yu

doi:10.1007/s00122-002-1145-4

Cited by 4 publications

(6 citation statements)

References 40 publications

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“…A similar expression profile of all sHSP-CI genes at a lower level was suggested by ethanol, H 2 O 2 and CuCl 2 treatments (Guan et al, 2004 (Guan et al, 2003(Guan et al, , 2004. Chang et al (2007), for the first time, reported a fulllength cDNA clone for a class II sHSP, isolated from riceetiolated seedlings heat shocked at 41 o C for 2 h. The coding sequence consists of 501 bp, and the clone encodes a protein of 18.0 kDa.…”

Section: Hsp60 Familysupporting

confidence: 57%

“…The DRs contain 123-bp regions with 99% nucleotide identity to the 5' coding region of the Oshsp16.9B gene. This fragment in proximity 5'-upstream of the OsHSP16.9B gene that was mapped on chromosome 1 with other two class-I sHSP genes, OsHSP16.9A and OsSHP16.9 C. Comparative analysis of the nucleotide sequences of class-I sHSP genes clustered on chromosome 1 showed that only the promoter region of OsHSP16.9B was different (Guan et al, 2003).…”

Section: Shsp Promotersmentioning

confidence: 89%

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An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

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In the last 25 years, a huge amount of literature has been accumulated describing the cell's response to different kinds of environmental stress conditions, such as high temperatures, altered pH, exposure of the cell to toxins, starvation, oxygen, and water deprivation, among others. Heat shock proteins (HSPs) are one of the main expressed products of the cell in response to stresses. HSPs can be classified into six structurally conserved classes according to their molecular weight namely, HSP100, HSP90, HSP70, HSP60, small heat shock proteins (sHSPs) and ubiquitin (8.5 kDa). In eukaryotes, different heat shock genes are expressed uncoordinatedly, whereas in prokaryote, heat shock genes form a regulon and appear simultaneously. sHSPs are associated with nuclei, cytoskeleton and membranes. They bind partially to denatured proteins, preventing irreversible protein aggregation during stress. In animals, only one sHSP gene has been located in yeast cells, ten in mammalian, two in birds and four genes have been found in Drosophila. However, in plants more than 20 sHSPs have been reported and they can be divided into 6 classes, of which, 3 classes (CI, CII and CIII) are in the cytosole or in the nucleus and the other three (CIV, CV and CVI) in the plastids, endoplasmic reticulum and mitochondria. Mitochondrial and chloroplast sHSPs protect electron transport chain. During development in animals, sHSP genes are normally regulated at late neurula and early tailbud stage and in plants during pollen development, seed maturation, seed imbibition and germination. Transcriptional regulation of sHSPs depends on particular activation of heat shock factors (HSF) which recognize the highly conserved heat-shock elements (HSEs). After the heat stress has been released, the sHSPs are quite stable, suggesting that sHSPs may be important for recovery as well.

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Section: Hsp60 Familysupporting

confidence: 57%

Section: Shsp Promotersmentioning

confidence: 89%

An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

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“…In our previous studies, we identified seven sHSP-CI genes in rice using rice Oshsp16.9A as a screening probe Lee et al, 1995;Chang et al, 2001;Guan et al, 2003). These genes were located on the rice chromosomes by RFLP using gene-specific 3¢-UTRs.…”

Section: Rice Shsp-ci Gene Family Contains Nine Membersmentioning

confidence: 99%

“…Because of the distinct abundance and complexity of sHSP-CI in rice, much research has concentrated on the identification of sHSP-CI genes in our laboratory (Tseng et al, 1993;Tzeng et al, 1993;Lee et al, 1995;Chang et al, 2001;Guan et al, 2003). In this report, we identified and characterized nine members of the rice sHSP-CI gene family on chromosome 1 and 3 and examined during seed maturation and the effects of various stresses including HS, Aze, As, Cd, and ethanol on expression profiles of these genes in etiolated seedlings.…”

Section: Introductionmentioning

confidence: 98%

Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.)

et al. 2004

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The cytosolic class I small heat shock proteins (sHSP-CI) represent the most abundant sHSP in plants. Here, we report the characterization and the expression profile of nine members of the sHSP-CI gene family in rice (Oryza sativa Tainung No.67), of which Oshsp16.9A, Oshsp16.9B, Oshsp16.9C, Oshsp16.9D and Oshsp17.9B are clustered on chromosome 1, and Oshsp17.3, Oshsp17.7, Oshsp17.9A and Oshsp18.0 are clustered on chromosome 3. Oshsp17.3 and Oshsp18.0 are linked by a 356-bp putative bi-directional promoter. Individual gene products were identified from the protein subunits of a heat shock complex (HSC) and from in vitro transcription/ translation products by two-dimensional gel electrophoreses (2-DE). All sHSP-CI genes except Oshsp17.9B were induced strongly after a 2-h heat shock treatment. The genes on chromosome 3 were induced rapidly at 32 and 41 degrees C, whereas those on chromosome 1 were induced slowly by similar conditions. Seven of these genes, except Oshsp16.9D and Oshsp17.9B, were induced by arsenite (As), but only genes on chromosome 3 were strongly induced by azetidine-2-carboxylic acid (Aze, a proline analog) and cadmium (Cd). A similar expression profile of all sHSP-CI genes at a lower level was evoked by ethanol, H2O2 and CuCl2 treatments. Transient expression assays of the promoter activity by linking to GUS reporter gene also supported the in vivo selective expression of the sHSP-CI genes by Aze treatment indicating the differential induction of rice sHSP-CI genes is most likely regulated at the transcriptional level. Only Oshsp16.9A abundantly accumulated in mature dry seed also suggested additionally prominent roles played by this HSP in development.

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“…Chromosomal mapping was conducted according to the method of (Guan et al 2003). On the basis of 196 RFLP loci, molecular linkage map was developed and data were scored as described by Wang et al (1998).…”

Section: Chromosome Mappingmentioning

confidence: 99%

Comparative analysis of nine different small heat shock protein gene promoters in Oryza sativa L. subsp. indica

et al. 2016

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Small heat shock proteins (sHSPs), the intra cellular chaperons respond to high temperatures and other stresses, are expressed under the control of the heat shock factors, which recognize highly conserved cis-regulatory elements (CREs). The present study was designed to identify and compare nine different sHSP gene promoters from rice on the basis of their location on chromosomes, sequence similarities, evolutionary relationship and putative binding sites of transcription factors. Additionally, they were analyzed for their functional importance in the regulation of sHSP genes. For this, 5 0 upstream region of nine sHSP genes from Oryza sativa was amplified, sequenced and analyzed. RFLP mapping approach using molecular linkage maps of three-way cross-population showed that OsHSP 16.9A, OsHSP 16.9B, OsHSP 16.9C and OsHSP C-II genes were clustered on the short arm of chromosome 1 with a 1238-bp overlapped sequence between OsHSP 16.9A and OsHSP 16.9B, whereas OsHSP 17.3, OsHSP 17.7, OsHSP 17.9, OsHSP 18.0 and OsHSP 26 genes were clustered on chromosome 3 with a 420-bp overlapped sequence between OsHSP 17.3 and OsHSP 18.0. Evolutionary analysis revealed that sHSPs that clustered on different chromosomes in the same specie showed high sequence similarity and evolved prior to the divergence of their localization. Eighteen CREs remained conserved among all sHSP gene promoters during evolution. These common regulatory elements are related to disease resistance, hormonal responses, light responses, seed storage proteins, tissue-specific expression, pollen development and other environmental stresses. Moreover, CCAATBOX1 was also found in all sHSP gene promoters involved in the regulation of heat shock response. Conclusively, promoter region of sHSP genes seems to be capable of driving genes expression during stress and genetic manipulation might be helpful in producing better quality rice.

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Characterization of a unique genomic clone located 5′ upstream of the Oshsp16.9B gene on chromosome 1 in rice (Oryza sativa L. cv Tainung No. 67)

Cited by 4 publications

References 40 publications

An overview on the small heat shock proteins

An overview on the small heat shock proteins

Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.)

Comparative analysis of nine different small heat shock protein gene promoters in Oryza sativa L. subsp. indica

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