Characterization and kinetics of lipase inhibitory Activity of streptomyces tendae

Singh, Gurmeet Kaur Surindar; Bayineni, Venkata Krishna; Kadeppagari, Ravi-Kumar

doi:10.22376/ijpbs.2017.8.2.b270-274

Cited by 4 publications

(7 citation statements)

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“…Streptomyces was reported to exert significant contribution to pancreatic lipase inhibition. Singh et al (2017) reported that S. tendae was found to be active as pancreatic lipase inhibitor with IC 50 value of 147.58 µg mL -1 , which was higher than Orlistat as positive control (IC 50 value of 0.65 µg mL -1 ). Other cases showed that pancreatic lipase inhibitor from Streptomyces soil isolate which showed 61.67% with 50 mg mL -1 butanol extract concentration (Kekuda et al, 2011).…”

Section: Ethyl Acetate Extract Inhibition Of Potential Endophytic Actinobacteria and Its Phytochemical Compoundsmentioning

confidence: 95%

“…Meanwhile, three other isolates RZP 1.3, RSS 2.1, and RSSB 3.2 were closely related to S. tendae and S. rubrogriseus (99.90%). Recently, S. tendae have been reported to have potential as pancreatic lipase inhibitors (Singh et al, 2017). Species that have similarity of > 99.0% to S. tendae showed to have antimicrobial activity (Laidi et al, 2006).…”

Section: Molecular Identity Of Potential Endophytic Actinobacteria Based On 16s Rrna Genementioning

confidence: 99%

“…Butanol extract from S. variabilis strains PO-178 could be used as pancreatic lipase inhibitor with IC 50 value of 44.31 mg mL -1 (Kekuda et al, 2014). Streptomyces tendae is also recognized as of pancreatic lipase inhibitor with IC 50 value of 147.58 µg mL -1 (Singh et al, 2017). Ethyl acetate extract of endophytic actinobacteria from Rhododendron spp.…”

Section: Molecular Identity Of Potential Endophytic Actinobacteria Based On 16s Rrna Genementioning

confidence: 99%

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Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.

2021

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Antiobesity medication is available as therapeutic compounds that can reduce fat digestion by the inhibition of pancreatic lipase. Actinobacteria have the potency as source of bioactive compounds with various biological function including as pancreatic lipase inhibitor. However, the potency of endophytic actinobacteria from Rhododendron spp. as source of pancreatic lipase inhibitor producer has not been reported yet. The aim of this study was to examine the potential of pancreatic lipase inhibitory activity of 23 endophytic actinobacteria from Rhododendron spp.; to characterize their colony based on morphology and molecular analysis. Screening test of pancreatic lipase inhibitor was conducted using the supernatant of endophytic actinobacteria, lipase pancreatic porcine (L3126) and p-nitrophenyl butyrate. The supernatant of selected isolates was extracted using ethyl acetate. The result showed that various inhibitory activities ranging between 0.00 until 91.69%. There were 11 out of 23 isolates that have potential as pancreatic inhibitor. Amongst them, the extract of four selected isolates, i.e. RZP 1.3, RSSB 3.2, RSS 2.1, and RJB F3.2 demonstrated inhibitory percentage of more than 80%. The RJB F3.2 extract showed to have IC50 value by 431.48 µg mL-1 compared to control, i.e. Xenical (89.07 µg mL-1). Phytochemical analysis exhibited that the extract of the selected isolates contained alkaloid which may function as pancreatic lipase inhibitor. Based on the morphological character, the selected isolates have various morphological colonies and 16S rRNA gene sequence revealed the sequence homology to Streptomyces spp. The data clearly indicate that endophytic actinobacteria from Rhododendron spp. have potency as pancreatic lipase inhibitor producer and further studies could be explored for the development of antiobesity agent.

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Section: Ethyl Acetate Extract Inhibition Of Potential Endophytic Actinobacteria and Its Phytochemical Compoundsmentioning

confidence: 95%

Section: Molecular Identity Of Potential Endophytic Actinobacteria Based On 16s Rrna Genementioning

confidence: 99%

Section: Molecular Identity Of Potential Endophytic Actinobacteria Based On 16s Rrna Genementioning

confidence: 99%

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Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.

2021

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“…The antidiabetic and antilipase activities of Jurinea members are known to be related with the phytochemical contents of the plants in question (Singh et al 2015, Singh et al 2016, Trendafilova et al 2018). In the present study, we aimed to investigate the α-amylase, αglucosidase and lipase inhibitory activities of J. brevicaulis to reveal its antiobesity potential and to determine the volatile and phenolic contents of the plant by using Solid-phase microextraction Gas Chromatography-Mass Spectrometry (SPME-GC/MS) and RP-HPLC techniques, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Triterpenes, a large structurally diverse group of natural compounds, display various biological activities including antitumor, antibacterial, antiviral, antiinflammatory and anti-diabetic (Zhang et al 2017). Bioactive molecules including saponins, phenols, terpenes, glycosides, alkaloids, carotenoids and polysaccharides from natural resources have been known to exhibit lipase inhibitory activity (Singh et al 2015). For instance, lipase inhibitory activity was reported for the chloroform extract and fractions of Jurinea tzarferdinandii Davidov, and the fractions were demonstrated to contain triterpenes, flavonoids and sesquiterpene lactones (Trendafilova et al 2018).…”

Section: Introductionmentioning

confidence: 99%

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Şener

Kanbolat

Çolak

et al. 2023

Trakya University Journal of Natural Sciences

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Obesity, defined as new world syndrome, is an epidemic that causes global health problems and big economic losses. Natural products have been gain increasing importance for the epidemic because of their antiobesity potency. Jurinea genus comprised approximately 200 species worldwide and has been used as a therapeutic agent for colic, fever, gout, and rheumatism, traditionally. The aim of this study was to analyze the volatile components, to determine its phenolic compounds and to evaluate its antiobesity potentcy of Jurinea brevicaulis BOISS. A widespread method based on solid-phase microextraction technique (SPME) was employed for GC-MS analysis of volatile components of J. brevicaulis. Quantitative analysis of phenolic compounds of J. brevicaulis was performed using RP-HPLC. To evaluate the antiobesity effect, the inhibitory effects of α-amylase, α-glucosidase, and lipase of the species were determined. A total of 19 volatile components were specified and o-cymene (10.60 %), β-Bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to HPLC analysis, sinapic acid, p-coumaric acid, and quercetin were determined for the species. IC50 value of the species was determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found 50.31 ± 3.75 μg/mL, according to lipase inhibition studies. Considering all the findings, it has been determined that the species is included diverse volatile components, antiobesity effective three phenolic compounds, and showed promising antiobesity potential so J. brevicaulis may be the up-and-coming candidate of natural product for obesity.

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ANTILIPASE ACTIVITY AND CHEMICAL CONSTITUENTS OFSolanum aculeatissimum Jacq. FRUITS

Megwee

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The nineteen Thai medicinal plants were investigated for anti-lipase activity. These plants were extracted with methanol and DI water. The methanolic and aqueous crude extracts of these plants were evaluated on anti-lipase activity. It was found that the methanolic extract of fruits of Solanum aculeatissimum showed the strongest inhibition of lipase activity. Thus, methanolic crude extract of S. aculeatissimum fruits were extracted by liquid-liquid extraction to obtain hexane, ethyl acetate, n-butanol and aqueous crude extracts. The hexane and n-butanol crude extracts of S. aculeatissimum fruits showed strong lipase inhibitory activity at a concentration of 25 mg/mL with percent inhibition as 81.88 ± 0.98% and 80.74 ± 1.39%, respectively. The ethyl acetate crude extract of S. aculeatissimum fruits showed moderate lipase inhibitory activity at a concentration of 25 mg/mL with percent inhibition as 60.41 ± 7.36%. Aqueous crude extract showed weak activity at a concentration of 25 mg/mL with percent inhibition as 22.12 ± 1.29%. The hexane and n-butanol crude extracts were further isolated to obtain six compounds. The hexane crude extract was isolated to give a mixture of fatty acids (compound 209) and steroidal mixture (compound 210). The n-butanol crude extract was isolated to give a mixture of steroidal glycosides (compound 211), solasodine (compound 212), solasonine (compound 213) and alpha-solanine (compound 214). Compounds 209 - 212 showed weak lipase inhibitory activity with IC50 values of 523.30 ± 0.91, 675.58 ± 1.29, 565.06 ± 1.27 µg/mL and 404.26 ± 1.39 µM, respectively. Compounds 213 and 214 exhibited moderate lipase inhibitory activity with IC50 values of 198.75 ± 0.96 and 223.43 ± 0.16 µM, respectively. Orlistat showed lipase inhibitory activity with IC50 value of 0.27 ± 0.07 µM. Compounds 209 - 214 exhibited in ranging of weak to moderate lipase inhibitory activity whereas hexane and n-butanol crude extracts showed strong lipase inhibitory. These results suggested that the chemical constituents of S. aculeatissimum fruits should be further investigated for the active components responsible for anti-lipase activity.

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Characterization and kinetics of lipase inhibitory Activity of streptomyces tendae

Cited by 4 publications

References 4 publications

Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.

Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

ANTILIPASE ACTIVITY AND CHEMICAL CONSTITUENTS OFSolanum aculeatissimum Jacq. FRUITS

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