Characterization and Genome Analysis of a Novel Mu-like Phage VW-6B Isolated from the Napahai Plateau Wetland of China

Cui, Zihong; Xu, Zhiwei; Wei, Yunlin; Zhang, Qi; Qin, Kunhao; Ji, Xiuling

doi:10.1007/s00284-020-02277-9

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…Wetlands occupy only 4-6% of the land surface [15], but store 12% of the world's soil organic carbon (SOC), therefore, wetlands play an important role in regulating the global carbon balance of terrestrial ecosystems [16]. The Napahai plateau wetland in northwest Yunnan belongs to the Jinsha River system, is a unique low-latitude, high-altitude seasonal plateau marsh wetland (altitude 3260 m, N 27°7′58″ ~27°55′ 00″) [17], and is classified as a wetland of international importance, making it a unique representative plateau wetland in China [18]. Due to rich species and diverse habitats (e.g., wintering habitats of migratory birds such as black-necked cranes), it is also one of the global hotspots for biodiversity conservation [19] with high scientific research value.…”

Section: Introductionmentioning

confidence: 99%

Microbial‐driven carbon fixation in natural wetland

Xiong

Zeng

et al. 2023

J Basic Microbiol

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With the development of global industrialization, carbon neutrality has become an issue that we must be paid attention to. Microorganisms not only have an important impact on the carbon chemical cycle between the Earth's biosphere and biogeography but also play a key role in maintaining the global organic carbon balance. Wetlands are the main reservoir of organic carbon in the mainland of China, and wetland carbon sinks are indispensable for China to achieve the goal of “dual carbon,” and China has taken the consolidation and improvement of wetland carbon sink capacity as an important part of the carbon peaking action plan. As a unique low‐latitude, high‐altitude seasonal plateau wetland in China, Napahai shows high research value. However, the role of microbes in maintaining dissolved organic carbon balance in this area has not been reported. In the study, six carbon fixation genes, accA, aclB, acsA, acsB, cbbL, and rbcL, were analyzed based on metagenomics to elucidate the rich genetic diversity, uniqueness and differences in the Napahai plateau wetland. It was found that the microbial diversity in the Napahai plateau wetland was different from other habitats. In addition, the aclB gene, a rare taxon with high genetic diversity and rich species in the Napahai plateau wetland, played a key role in the microbial metabolic pathway. Finally, the construction of a metabolic pathway through the Kyoto encyclopedia for genes and genomes revealed the contribution of microbes to carbon fixation and the role of microbes in maintaining the organic carbon balance of the Napahai plateau wetland.

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Section: Introductionmentioning

confidence: 99%

Microbial‐driven carbon fixation in natural wetland

Xiong

Zeng

et al. 2023

J Basic Microbiol

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“…Located in Jiantang Town, Shangri‐La City, Diqing Prefecture, the Napahai plateau wetland is a natural species resource bank of alpine organisms. Due to its rich species and diverse habitats (such as the wintering habitat of black‐necked cranes), it is also one of the hotspots of global biodiversity conservation [20] and has high scientific research value. Based on the unique geographical location of the Napahai plateau wetland, we suspect that the virus AMGs may have different genes from different habitats, and the hosts on which the virus depends will also be different due to the environment.…”

Section: Introductionmentioning

confidence: 99%

Viral AMGs‐driven pentose phosphate pathway in natural wetland

Li,

Yu,

Xiong

et al. 2023

J Basic Microbiol

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Viruses exist anywhere on earth where there is life, and among them, virus‐encoded auxiliary metabolic genes (AMGs) can maintain ecosystem balance and play a major role in the global ecosystem. Although the function of AMGs has been widely reported, the genetic diversity of AMGs in natural ecosystems is still poorly understood. Exploring the genetic diversity of viral community‐wide AMGs is essential to gain insight into the complex interactions between viruses and hosts. In this article, we studied the phylogenetic tree, principal co‐ordinates analysis (PCoA), α diversity, and metabolic pathways of viral auxiliary metabolism genes involved in the pentose phosphate pathway (PPP) through metagenomics, and the changes of metabolites and genes of host bacteria were further studied by using Pseudomonas mandelii SW‐3 and its lytic phage based on metabolic flow and AMGs expression. We found that the viral AMGs in the Napahai plateau wetland were created by a combination of various external forces, which contributed to the rich genetic diversity, uniqueness, and differences of the virus, which promoted the reproduction of offspring and better adaptation to the environment. Overall, this study systematically describes the genetic diversity of AMGs associated with the PPP in plateau wetland ecosystems and further expands the understanding of phage–host unique interactions.

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Function and distribution of nitrogen-cycling microbial communities in the Napahai plateau wetland

Li,

Xiong,

et al. 2023

Arch Microbiol

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Characterization and Genome Analysis of a Novel Mu-like Phage VW-6B Isolated from the Napahai Plateau Wetland of China

Cited by 5 publications

References 42 publications

Microbial‐driven carbon fixation in natural wetland

Microbial‐driven carbon fixation in natural wetland

Viral AMGs‐driven pentose phosphate pathway in natural wetland

Function and distribution of nitrogen-cycling microbial communities in the Napahai plateau wetland

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