Characterization and Genetic Analysis of a Novel Rice Spotted‐leaf Mutant HM47 with Broad‐spectrum Resistance to <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>

Feng, Bo; Yang, Yang; Shí, Yùzhēn; Shen, Hai-Chao; Wang, Huimei; Huang, Qi-na; Xu, Xiaogang; Lü, Xiang-guang; Wu, Jianli

doi:10.1111/jipb.12021

Cited by 40 publications

(41 citation statements)

References 56 publications

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“…We believe that the identification of the rice lmr mutant and isolation of the corresponding LMR gene provides an additional tool to facilitate the elucidation of the molecular mechanisms underlying PCD in plants in general and in monocots in particular, because the defense response involving PCD is less understood in monocots compared with the mechanisms in dicots (Matin et al 2010). Although more than 55 rice lesion mutants were characterized in rice (Feng et al 2013), only a few of the corresponding genes have been cloned to date. About 8 genes associated with LMMs have been isolated in rice using different cloning strategies (Yamanouchi et al 2002;Zeng et al 2004;Wang et al 2005;Mori et al 2007;Takahashi et al 2007;Qiao et al 2010;Sun et al 2011), and cloning of the LESION MIMIC AND SENESCENCE (LMS) gene B encoding a protein with two double-stranded RNA binding motifs has been reported recently (Undan et al 2012).…”

Section: Discussionmentioning

confidence: 98%

The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response

et al. 2014

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Lesion mimic mutants (LMMs) provide a useful tool to study defense-related programmed cell death (PCD) in plants. Although a number of LMMs have been identified in multiple species, most of the candidate genes are yet to be isolated. Here, we report the identification and characterization of a novel rice (Oryza sativa L.) lesion mimic resembling (lmr) mutant, and cloning of the corresponding LMR gene. The LMR locus was initially delineated to 1.2 Mb region on chromosome 6, which was further narrowed down to 155-kb using insertions/deletions (INDELs) and cleavage amplified polymorphic sequence markers developed in this study. We sequenced the open reading frames predicted within the candidate genomic region, and identified a G-A base substitution causing a premature translation termination in a gene that encodes an ATPase associated with various cellular activities type (AAA-type) protein. RNA interference transgenic lines with reduced LMR transcripts exhibited the lesion mimic phenotype similar to that of lmr plants. Furthermore, expression of the wild-type LMR in the mutant background complemented the lesion phenotype, confirming that the mutation identified in LMR is responsible for the mutant phenotype. The pathogenesis-related (PR) genes PBZ1 and PR1 were induced in lmr, which also showed enhanced resistance to rice blast (Magnaporthe oryzae) and bacterial blight (Xanthomonas oryzae pv. oryzae), suggesting LMR is a negative regulator of cell death in rice. The identification of lmr and cloning of the corresponding LMR gene provide an additional resource for the study of PCD in plants.

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Section: Discussionmentioning

confidence: 98%

The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response

et al. 2014

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“…Primers for qRT-PCR are listed in Supplementary Table 1. PCR and detection were performed as described previously (Feng et al, 2013). The 2 −ΔΔCT method was used to analyze the relative transcript levels in gene expression with the means from three replications.…”

Section: Methodsmentioning

confidence: 99%

Non-photochemical Quenching Plays a Key Role in Light Acclimation of Rice Plants Differing in Leaf Color

et al. 2017

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Non-photochemical quenching (NPQ) is an important photoprotective mechanism in rice; however, little is known regarding its role in the photosynthetic response of rice plants with differing in leaf color to different irradiances. In this study, two rice genotypes containing different chlorophyll contents, namely Zhefu802 (high chlorophyll) and Chl-8 (low chlorophyll), were subjected to moderate or high levels of light intensity at the 6-leaf stage. Chl-8 possessed a lower chlorophyll content and higher chlorophyll a:b ratio compared with Zhefu802, while Pn, Fv/Fm, and ΦPSII contents were higher in Chl-8. Further results indicated that no significant differences were observed in the activities of Rubisco, Mg2+-ATPase, and Ca2+-ATPase between these genotypes. This suggested that no significant difference in the capacity for CO2 assimilation exists between Zhe802 and Chl-8. Additionally, no significant differences in stomatal limitation were observed between the genotypes. Interestingly, higher NPQ and energy quenching (qE), as well as lower photoinhibitory quenching (qI) and production of reactive oxygen species (ROS) was observed in Chl-8 compared with Zhefu802 under both moderate and high light treatments. This indicated that NPQ could improve photosynthesis in rice under both moderate and high light intensities, particularly the latter, whereby NPQ alleviates photodamage by reducing ROS production. Both zeaxanthin content and the expression of PsbS1 were associated with the induction of NPQ under moderate light, while only zeaxanthin was associated with NPQ induction under high light. In summary, NPQ could improve photosynthesis in rice under moderate light and alleviate photodamage under high light via a decrease in ROS generation.

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“…Activation of the SA response causes disease resistance to bacterial leaf blight pathogen in pelota Our previous studies have shown that rice pelota is broadly resistant to Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial leaf blight in rice (Feng et al 2013). Although resistance to Xoo is highly associated with the spotted-leaf phenotype and a couple of defense genes were found to be upregulated in pelota (Feng et al 2013), the resistance mechanism was still unclear.…”

Section: Resultsmentioning

confidence: 99%

“…The rice pelota shows impaired growth, spotted‐leaf, and broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae (Feng et al ).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, a rice spotted‐leaf mutant obtained from ethyl methanesulfonate (EMS) mutagenesis was characterized and the causal gene locus was narrowed down to a 74 kb region in chromosome 4 by map‐based cloning (Feng et al ). Here, we show that the rice PELOTA protein (OsPELOTA), encoded by LOC_Os04g56480 , controls the spotted‐leaf phenotype.…”

Section: Introductionmentioning

confidence: 99%

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A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

Zhang

Feng

Wang

et al. 2018

JIPB

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We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the mapbased cloning of the causal gene OsPELOTA (originally termed spl HM47). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.

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Characterization and Genetic Analysis of a Novel Rice Spotted‐leaf Mutant HM47 with Broad‐spectrum Resistance to Xanthomonas oryzae pv. oryzae

Cited by 40 publications

References 56 publications

The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response

The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response

Non-photochemical Quenching Plays a Key Role in Light Acclimation of Rice Plants Differing in Leaf Color

A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

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