Characterization and full genome sequence analysis of a Chinese isolate of tomato chlorosis virus

Zhao, Ruipeng; N, Wang; Wang, R; Chen, H; Shí, Yùzhēn; Zhao, Fan; Zhou, Tong

doi:10.4149/av_2014_01_92

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…To date, 17 complete ToCV genome sequences have been published, one each from the USA (Wintermantel et al , ), Spain (Lozano et al , , ), Greece (Kataya et al , ), Brazil (Albuquerque et al , ) and Taiwan (Kang et al , ), two from China (Zhao et al , , 2014b) and ten from Korea (Lee et al , ).…”

Section: Genome Organization and Diversitymentioning

confidence: 99%

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

Fiallo‐Olivé

Navas‐Castillo

2019

Molecular Plant Pathology

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Summary Tomato chlorosis virus (ToCV) causes an important disease that primarily affects tomato, although it has been found infecting other economically important vegetable crops and a wide range of wild plants. First described in Florida (USA) and associated with a ‘yellow leaf disorder’ in the mid‐1990s, ToCV has been found in 35 countries and territories to date, constituting a paradigmatic example of an emergent plant pathogen. ToCV is transmitted semipersistently by whiteflies (Hemiptera: Aleyrodidae) belonging to the genera Bemisia and Trialeurodes . Whitefly transmission is highly efficient and cases of 100% infection are frequently observed in the field. To date, no resistant or tolerant tomato plants are commercially available and the control of the disease relies primarily on the control of the insect vector. Taxonomy Tomato chlorosis virus is one of the 14 accepted species in the genus Crinivirus , one of the four genera in the family Closteroviridae of plant viruses. Virion and genome properties The genome of ToCV is composed of two molecules of single‐stranded positive‐sense RNA, named RNA1 and RNA2, separately encapsidated in long, flexuous, rod‐like virions. As has been shown for other closterovirids, ToCV virions are believed to have a bipolar structure. RNA1 contains four open reading frames (ORFs) encoding proteins associated with virus replication and suppression of gene silencing, whereas RNA2 contains nine ORFs encoding proteins putatively involved in encapsidation, cell‐to‐cell movement, gene silencing suppression and whitefly transmission. Host range In addition to tomato, ToCV has been found to infect 84 dicot plant species belonging to 25 botanical families, including economically important crops. Transmission Like all species within the genus Crinivirus , ToCV is semipersistently transmitted by whiteflies, being one of only two criniviruses transmitted by members of the genera Bemisia and Trialeurodes . Disease symptoms Tomato ‘yellow leaf disorder’ syndrome includes interveinal yellowing and thickening of leaves. Symptoms first develop on lower leaves and then advance towards the upper part of the plant. Bronzing and necrosis of the older leaves are accompanied by a decline in vigour and reduction in fruit yield. In other hosts the most common symptoms include interveinal chlorosis and mild yellowing on older leaves. Control Control of the disease caused by ToCV is based on the use of healthy seedlings for transplanting, limiting accessibility of alternate host plants that can serve as virus reservoirs and the spraying of insecticides for vector control. Although several wild tomato species have been sh...

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Section: Genome Organization and Diversitymentioning

confidence: 99%

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

Fiallo‐Olivé

Navas‐Castillo

2019

Molecular Plant Pathology

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“…The chlorotic leaf symptom of tomatoes was observed in Florida in 1989, and the virus responsible was first named Tomato chlorosis virus (ToCV) in the USA ( Wisler et al, 1998b ). Subsequently, this virus has been distributed to many parts of the world including European, American, African and Asian countries ( Abou-Jawdah et al, 2006 ; Accotto et al, 2001 ; Alvarez-Ruiz et al, 2007 ; Arruabarrena et al, 2015 ; Barbosa et al, 2008 ; Bese et al, 2011 ; Castro et al, 2009 ; Çevik and Erkıß, 2008 ; Dalmon et al, 2005 ; Dovas et al, 2002 ; Fiallo-Olivé et al, 2011 ; Hirota et al, 2010 ; Jacquemond et al, 2009 ; Lett et al, 2009 ; Louro et al, 2000 ; Segev et al, 2004 ; Sundaraj et al, 2011 ; Wintermantel et al, 2001 ; Wintermantel and Wisler, 2006 ; Zhao et al, 2013a ). In northeast Asia, ToCV occurrence was originally reported in China, Taiwan and Japan ( Hirota et al, 2010 ; Tsai et al, 2004 ; Zhao et al, 2013b ), but was not reported in Korea until 2013.…”

mentioning

confidence: 99%

Phylogenetic Characterization of Tomato chlorosis virus Population in Korea: Evidence of Reassortment between Isolates from Different Origins

et al. 2018

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“…Viral genomic RNAs were prepared from purified virions. Specific primers were designed based on the complete genome sequence of ToCV-BJ (accession number KC887998 and KC887999) (17). First strand cDNA was synthesized with moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, USA) according to the manufacturer's protocol.…”

mentioning

confidence: 99%

“…To develop an infectious cDNA clone of ToCV Beijing isolate (ToCV-BJ) (17), reverse transcription and polymerase chain reactions (RT-PCR) were used to synthesize and amplify full-length cDNA of ToCV-BJ RNA 1 and 2. Viral genomic RNAs were prepared from purified virions.…”

mentioning

confidence: 99%

P22 of tomato chlorosis virus, an RNA silencing suppressor, is naturally expressed in the infected plant

Zhao¹,

N²,

Liu³

et al. 2016

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Characterization and full genome sequence analysis of a Chinese isolate of tomato chlorosis virus

Cited by 5 publications

References 22 publications

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

Tomato chlorosis virus, an emergent plant virus still expanding its geographical and host ranges

Phylogenetic Characterization of Tomato chlorosis virus Population in Korea: Evidence of Reassortment between Isolates from Different Origins

P22 of tomato chlorosis virus, an RNA silencing suppressor, is naturally expressed in the infected plant

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