P22 of tomato chlorosis virus, an RNA silencing suppressor, is naturally expressed in the infected plant

Zhao, Ruqian; N, Wang; Liu, S; Ling, Kai-Shu; Zhao, Fan; Zhou, Tao

doi:10.4149/av_2016_04_423

Cited by 12 publications

(16 citation statements)

References 18 publications

(23 reference statements)

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“…The infectious cDNA clone of ToCV was provided by Prof. Tao Zhou (China Agricultural University). The preparation of ToCV-infected tomato plants were performed as described ( Zhao et al, 2016 ). First, plasmids containing RNA1 (pCa-ToCR1) and RNA2 (pCa-ToCR2) were placed respectively in two liquid YEP mediums containing 50 mg/mL kanamycin and 50 mg/mL rifampicin.…”

Section: Methodsmentioning

confidence: 99%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.

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Section: Methodsmentioning

confidence: 99%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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“…Zhongza 9) were kept in whitefly-proof screen-cages in a greenhouse. To achieve systemic infection, plants at the three-true-leaf stage were injected with an infectious ToCV cDNA clone, and 0.5 ml of the infectious cDNA agro clone was injected into each plant (Zhao et al, 2016 ). The infection of was confirmed by the symptoms (chlorotic leaves) and by molecular detection at least 30 days post-inoculation using reverse transcription-polymerase chain reaction (RT-PCR).…”

Section: Methodsmentioning

confidence: 99%

Transmission Efficiency, Preference and Behavior of Bemisia tabaci MEAM1 and MED under the Influence of Tomato Chlorosis Virus

et al. 2018

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Tomato chlorosis virus (ToCV, genus Crinivirus, family Closteroviridae) is an economically important virus in more than 20 countries. In China, ToCV was first detected in 2013 and has already spread throughout the country. ToCV is transmitted in a semi-persistent manner by the whitefly, Bemisia tabaci, but not seed. In the past two decades, the most invasive MEAM1 and MED have replaced the indigenous B. tabaci in China, and currently MED is the most dominant cryptic species. To better understand the prevalence of ToCV with their vectors, we tested the hypothesis that the rapid spread of ToCV in China is closely related to the dominance of MED. ToCV acquisition and accumulation rate following transmission was significantly higher by MED than MEAM1. In addition, ToCV persisted for more than 4 days in MED but only 2 days in MEAM1. Viruliferous MED preferred non-infected over virus-infected plants, although MED performed better on infected than on non-infected plants. Our combined results support the initial hypothesis that the rapid spread of ToCV is associated with the spread of B. tabaci MED in China.

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“…Subsequently, the infectious virions can be transferred to the natural hosts to initiate an infection by bark-flap or stem slash inoculation (CTV; Gowda et al, 2005;Ambrós et al, 2011), whitefly transmission (LIYV; Wang et al, 2009), or grafting (ToCV; Orílio et al, 2014). It is worth mentioning that the cDNA clones generated for a Chinese ToCV isolate are also agroinfectious in N. benthamiana (Zhao et al, 2016) but not in tomato (Tao Zhou, personal communication). Wang et al (2009) suggested a number of factors that could contribute to the inability of agroinfectious viral clones to accomplish systemic infection in the natural host plants, including transcript splicing, thus reducing the functional RNA transcripts reaching the cytoplasm, the difference in virulence conferred by different A. tumefaciens strains, the plant defense responses caused by the combination of specific A. tumefaciens strains and viruses, the inaccessibility of phloem cells, or a strong silencing response at the very early stages of infection.…”

Section: Tocv Clones Containing the Hdv Ribozyme Are Still Not Agroinfectious In Tomatomentioning

confidence: 99%

Infectious Clones of Tomato Chlorosis Virus: Toward Increasing Efficiency by Introducing the Hepatitis Delta Virus Ribozyme

2021

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Tomato chlorosis virus (ToCV) is an emergent plant pathogen that causes a yellow leaf disorder in tomato and other solanaceous crops. ToCV is a positive-sense, single stranded (ss)RNA bipartite virus with long and flexuous virions belonging to the genus Crininivirus (family Closteroviridae). ToCV is phloem-limited, transmissible by whiteflies, and causes symptoms of interveinal chlorosis, bronzing, and necrosis in the lower leaves of tomato accompanied by a decline in vigor and reduction in fruit yield. The availability of infectious virus clones is a valuable tool for reverse genetic studies that has been long been hampered in the case of closterovirids due to their genome size and complexity. Here, attempts were made to improve the infectivity of the available agroinfectious cDNA ToCV clones (isolate AT80/99-IC from Spain) by adding the hepatitis delta virus (HDV) ribozyme fused to the 3′ end of both genome components, RNA1 and RNA2. The inclusion of the ribozyme generated a viral progeny with RNA1 3′ ends more similar to that present in the clone used for agroinoculation. Nevertheless, the obtained clones were not able to infect tomato plants by direct agroinoculation, like the original clones. However, the infectivity of the clones carrying the HDV ribozyme in Nicotiana benthamiana plants increased, on average, by two-fold compared with the previously available clones.

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P22 of tomato chlorosis virus, an RNA silencing suppressor, is naturally expressed in the infected plant

Cited by 12 publications

References 18 publications

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Transmission Efficiency, Preference and Behavior of Bemisia tabaci MEAM1 and MED under the Influence of Tomato Chlorosis Virus

Infectious Clones of Tomato Chlorosis Virus: Toward Increasing Efficiency by Introducing the Hepatitis Delta Virus Ribozyme

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