Characterization and evolutionary analysis of tributyltin‐binding protein and pufferfish saxitoxin and tetrodotoxin‐binding protein genes in toxic and nontoxic pufferfishes

Hashiguchi, Yasuyuki; Lee, J. M.; Shiraishi, Masayuki; Komatsu, S.; Miki, Shizuho; Shimasaki, Yohei; Mochioka, Noritaka; Kusakabe, Takahiro; Oshima, Yuji

doi:10.1111/jeb.12634

Cited by 29 publications

(29 citation statements)

References 54 publications

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“…Nevertheless, Tetraodontidae was not recommended for human consumption. Hashiguchi et al (2015) reported that family Tetraodontidae or pufferfish contained tetrodotoxin and saxitoxin in their organs and the degree of toxicity was highly variable even within each toxic species. Tetrodotoxin in the pufferfish localized in the ovary of its larvae as protection against predators (Itoi et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Protein and fatty acid profile of marine fishes from Java Sea, Indonesia

et al. 2018

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Priatni S, Ratnaningrum D, Kosasih W, Sriendah E, Srikandace Y, Rosmalina T, Pudjiraharti S. 2018. Protein and fatty acidprofile of marine fishes from Java Sea, Indonesia. Biodiversitas 19: 1737-1742. Indonesia is the second largest producer of capturefisheries products in the world and the most capture fisheries production comes from marine fisheries. Marine fish is a source of protein,amino acid, saturated and unsaturated fatty acids, which are important components of diet. The objective of the study was to investigatethe protein and fatty acids profile of nine marine fish samples from Java Sea of Indramayu West Java, Indonesia. The analysis datashowed that the total protein content of fish samples ranged from 61.07% (Pampus argenteus) to 86.56% (Tetraodontidae). Meanwhile,total lipid content of fish samples ranged from 1.73% (Tetraodontidae) to 9.82% (Leiognathus equulus). The concentration of α-AminoNitrogen (AN) of fish protein hydrolysate was ranging from 31 mM (Nemipterus hexodon) to 69 mM (Mystacoleucus padangensis)and% Degree of Hydrolysis (DH) was ranging from 9.33% to 20.39%. The molecular weight of protein fish samples had similar profilesprimarily for almost all samples, which could be observed from a typical band with the weight around 49 kDa. The saturated fatty acid(Ʃ SFA) compositions of fish species ranged from 1094.03-4233.03 μg/g. Oleic acid (MUFA) content of all fish species ranged from257.91-1216.06 μg/g. However, only three fish species contain of Poly Unsaturated Fatty Acid (PUFA) linoleic acid as the following;Selaroides leptolepis (171.36 μg/g), Oxyeleotris marmorata (249.40μg/g) and Tetraodontidae (140.35 μg/g). The highest SFA contentwas found in S. leptolepis with palmitic acid (C16:0) as the dominant saturated fatty acid (2320.88 μg/g). S. leptolepis also containedhigh oleic acid (1216.06 μg/g) and linoleic acid (171.36 μg/g).

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Section: Discussionmentioning

confidence: 99%

Protein and fatty acid profile of marine fishes from Java Sea, Indonesia

et al. 2018

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“…Therefore, PSTBP is assumed to be a carrier protein that transfers TTX among tissues in toxic species of pufferfish in the genus Takifugu . Hashiguchi et al [ 41 ] examined PSTBP genes in five toxic species of pufferfish in the genus Takifugu and three non-toxic species of pufferfish ( L. wheeleri (at present L. spadiceus ), L. gloveri (at present L. cheesemanii ) and S. pachygaster ). PSTBP genes were cloned in the genus Takifugu , while they were not obtained in the three non-toxic pufferfish.…”

Section: Discussionmentioning

confidence: 99%

Difference in Uptake of Tetrodotoxin and Saxitoxins into Liver Tissue Slices among Pufferfish, Boxfish and Porcupinefish

et al. 2018

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Although pufferfish of the family Tetraodontidae contain high levels of tetrodotoxin (TTX) mainly in the liver, some species of pufferfish, boxfish of the family Ostraciidae, and porcupinefish of the family Diodontidae do not. To clarify the mechanisms, uptake of TTX and saxitoxins (STXs) into liver tissue slices of pufferfish, boxfish and porcupinefish was examined. Liver tissue slices of the pufferfish (toxic species Takifugu rubripes and non-toxic species Lagocephalus spadiceus, L. cheesemanii and Sphoeroides pachygaster) incubated with 50 µM TTX accumulated TTX (0.99–1.55 µg TTX/mg protein) after 8 h, regardless of the toxicity of the species. In contrast, in liver tissue slices of boxfish (Ostracion immaculatus) and porcupinefish (Diodon holocanthus, D. liturosus, D. hystrix and Chilomycterus reticulatus), TTX content did not increase with incubation time, and was about 0.1 µg TTX/mg protein. When liver tissue slices were incubated with 50 µM STXs for 8 h, the STXs content was <0.1 µg STXs/mg protein, irrespective of the fish species. These findings indicate that, like the toxic species of pufferfish T. rubripes, non-toxic species such as L. spadiceus, L. cheesemanii and S. pachygaster, potentially take up TTX into the liver, while non-toxic boxfish and porcupinefish do not take up either TTX or STXs.

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“…The difference of toxin distribution pattern between Takifugu and Arothron is probably caused by the difference of some proteins which are implicated in TTX accumulation system. We previously found a glycoprotein, named as PSTBP (puffer fish saxitoxin and tetrodotoxin binding protein), that binds to TTX and STX in the blood plasma of Takifugu pardalis [ 51 ], and also, it was reported that PSTBP was commonly detected in several Takifugu species [ 52 , 53 ]. We are planning to examine the presence of PSTBP-like protein in Arothron species to get a hint that will help explain the difference of TTX-accumulation tissues between Takifugu and Arothron genus.…”

Section: Discussionmentioning

confidence: 99%

Tetrodotoxin and Its Analogues in the Pufferfish Arothron hispidus and A. nigropunctatus from the Solomon Islands: A Comparison of Their Toxin Profiles with the Same Species from Okinawa, Japan

Puilingi

Kudo

Cho

et al. 2015

Toxins

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Pufferfish poisoning has not been well documented in the South Pacific, although fish and other seafood are sources of protein in these island nations. In this study, tetrodotoxin (TTX) and its analogues in each organ of the pufferfish Arothron hispidus and A. nigropunctatus collected in the Solomon Islands were investigated using high resolution LC-MS. The toxin profiles of the same two species of pufferfish from Okinawa, Japan were also examined for comparison. TTXs concentrations were higher in the skin of both species from both regions, and relatively lower in the liver, ovary, testis, stomach, intestine, and flesh. Due to higher TTX concentrations (51.0 and 28.7 µg/g at highest) detected in the skin of the two species from the Solomon Islands (saxitoxin was <0.02 µg/g), these species should be banned from consumption. Similar results were obtained from fish collected in Okinawa, Japan: TTX in the skin of A. hispidus and A. nigropunctatus were 12.7 and 255 µg/g, respectively, at highest, and saxitoxin was also detected in the skin (2.80 µg/g at highest) and ovary of A. hispidus. TTX, 5,6,11-trideoxyTTX (with its 4-epi form), and its anhydro forms were the most abundant, and 11-oxoTTX was commonly detected in the skin.

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Characterization and evolutionary analysis of tributyltin‐binding protein and pufferfish saxitoxin and tetrodotoxin‐binding protein genes in toxic and nontoxic pufferfishes

Cited by 29 publications

References 54 publications

Protein and fatty acid profile of marine fishes from Java Sea, Indonesia

Protein and fatty acid profile of marine fishes from Java Sea, Indonesia

Difference in Uptake of Tetrodotoxin and Saxitoxins into Liver Tissue Slices among Pufferfish, Boxfish and Porcupinefish

Tetrodotoxin and Its Analogues in the Pufferfish Arothron hispidus and A. nigropunctatus from the Solomon Islands: A Comparison of Their Toxin Profiles with the Same Species from Okinawa, Japan

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