Characterization and determination of piperine and piperine isomers in eggs

Ternes, Waldemar; Krause, Edburga L.

doi:10.1007/s00216-002-1416-6

Cited by 41 publications

(41 citation statements)

References 14 publications

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“…The above signals are characteristic of piperine and distinguish it from its geometric isomers viz., chavicine, isopiperine, and isochavicine [13]. The 1 H NMR spectrum of the isolated compound did not contain signals characteristic of the geometric isomers of piperine, confirming the purity of the compound [13]. The purity of the isolated crude piperine, as determined by HPTLC, was found to be 90%.…”

Section: Resultsmentioning

confidence: 56%

A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.

et al. 2008

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A simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of Piper nigrum. The method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform and subsequently crystallized. The identity of the compound was confirmed by its melting point, comparison of UV, IR, and mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer chromatography (TLC). The purity of the compound was ascertained by TLC, by recording UV absorption spectra at the start, middle, and end positions of the spot on the plate, and by differential scanning calorimetry (DSC).

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Section: Resultsmentioning

confidence: 56%

A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.

et al. 2008

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“…Previous assays have described several methods for the determination of piperine in biological fluids, including HP-TLC [12], HPLC [13][14][15][16], LC-NMR-MS [17], LC-MS-MS [18], and UFLC [19]. These methods do not meet modern drug development needs with respect to an efficient extraction procedure, shorter run time and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous UFLC–ESI–MS/MS determination of piperine and piperlonguminine in rat plasma after oral administration of alkaloids from Piper longum L.: Application to pharmacokinetic studies in rats

Liu

Luo

et al. 2011

Journal of Chromatography B

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“…Assays described to date include high-performance liquid chromatography (HPLC), magnetic resonance spectroscopy, and mass spectroscopy methods (25–27). There are no published liquid chromatography/tandem mass spectrometry (LC/MSMS) methods for the assay of piperine from human plasma.…”

Section: Introductionmentioning

confidence: 99%

Ultra-low Flow Liquid Chromatography Assay with Ultraviolet (UV) Detection for Piperine Quantitation in Human Plasma

Kakarala

Dubey

Tarnowski

et al. 2010

J. Agric. Food Chem.

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A robust and sensitive ultra-low flow liquid chromatography (UFLC) method that can reproducibly, at reasonable cost, detect low concentrations of piperine from human plasma is necessary. Piperine in plasma was separated and quantified by a gradient method using ultraviolet detection at a maximal absorbance wavelength of 340 nm. An aliquot was injected onto a reversed-phase column Waters SymmetryShield, 2.1 × 100 mm, 3.5 μm, C18 column, attached to a Waters absorbosphere, 4.6 × 30 mm, C18 guard column and eluted with a mobile phase containing a mixture of acetonitrile/water/ acetic acid (25:74.9:0.1, v/v/v) on line A and acetonitrile/acetic acid (99.9:0.1, v/v) on line B. The flow rate was 0.3 mL/min. The gradient method consisted of an opening condition of 20% pump B, with a linear increase to 37% pump B over 8 min, then a linear increase to 100% pump B at 11 min, 2 min at 100% pump B, and then a return to the opening condition (20% pump B) via a linear gradient over 2 min, followed by 5 min re-equilibration at opening conditions. The total run time was 20 min for each sample. All samples were processed protected from ambient light to avoid isomerization of piperine. The plasma assay was linear with R = 0.9995, with a lower limit of detection [signal-to-noise (S/N) > 5:1] of 100 pg of piperine loaded into the analytical system with acceptable accuracy and precision. Extraction recoveries of piperine from human plasma were 88% for quality control high (QCH), 93% for quality control medium (QCM), and 90% for quality control low (QCL), and the matrix effect was <12%. Piperine was quantifiable from a 50 mg oral dose given to human volunteers. A UFLC method for the rapid assay of human plasma with sensitivity to detect as low as 5 ng/mL piperine was developed. The method sensitivity equals that of liquid chromatography/tandem mass spectrometry (LC/MSMS) methods with much less cost.

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Characterization and determination of piperine and piperine isomers in eggs

Cited by 41 publications

References 14 publications

A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.

A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.

Simultaneous UFLC–ESI–MS/MS determination of piperine and piperlonguminine in rat plasma after oral administration of alkaloids from Piper longum L.: Application to pharmacokinetic studies in rats

Ultra-low Flow Liquid Chromatography Assay with Ultraviolet (UV) Detection for Piperine Quantitation in Human Plasma

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