Simultaneous UFLC–ESI–MS/MS determination of piperine and piperlonguminine in rat plasma after oral administration of alkaloids from Piper longum L.: Application to pharmacokinetic studies in rats

Neuroprotective effects of alkaloids fromPiper longumin a MPTP-induced mouse model of Parkinson’s disease

“…The LD 50 value of piperine by intragastric administration is 330 mgkg À1 (Piyachaturawat et al, 1983), whereas PLA is 1509 mgkg À1 , which indicated that PLA is safer with lower toxicity. Pharmacokinetic experiments also demonstrated that the oral bioavailability of PLA is higher than piperine (Liu et al, 2011). Therefore, we speculate that PLA may have pleiotropic effects.…”

Protection effect of piperine and piperlonguminine from Piper longum L. alkaloids against rotenone-induced neuronal injury

“…In a previous study, the PLL alkaloids PIP and PLG were found to exert protective effects in a PD model (Liu et al, 2011). The current study investigated the mechanism underlying these effects by inducing a PD-like syndrome using rotenone, as evidenced by the diminished performance on the rotarod test and loss of dopaminergic neurons in the midbrain (Fig.…”

“…PLL alkaloids and pharmacological reagents PLLE and its main constituents PIP and PLG were obtained as previously described (Liu et al, 2011). Cyclosporin A (Sigma, St. Louis, MO, USA) was used to block the opening of the mPTP (Yarana et al, 2012) and served as a positive control for PLLE, while ruthenium red (RR,Sigma), which blocks mitochondrial influx, was used as negative control (Duzenli et al, 2005).…”

Quality control assessment of polyherbal formulation based on a quantitative determination multimarker approach by ultra high performance liquid chromatography with tandem mass spectrometry using polarity switching combined with multivariate analysis

“…All the compounds dependent MS parameters (precursor ion, product ion, declustering potential (DP) and collision energy (CE) were carefully optimized for each target compound individually, in both positive and negative ion modes, which was performed by FIA of the individual standard solution into the mass spectrometer. [31][32][33][34][35][36][37]. MRM parameters: DP, EP, CE and CXP were optimized to achieve the most abundant, specific and stable MRM transition for each compound as shown in Table S3 and the MS/MS spectra are shown in Fig.…”