Characterization and Determination of Antimicrobial Sensitivity Pattern of Staphylococcus aureus Associated with Urinary Tract Infection

Ali, Muhammad Asad; Diso, Sani U.; Zage, Abubakar U.; Muhammad, A. A.; Garba, M.

doi:10.9734/jabb/2017/31125

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…It was then allowed to cool down and the lid was removed. After 15 minutes, the peptone water was poured in sterile universal bottle while the media poured into sterile Petri dish and allowed to solidify at room temperature (14) .…”

Section: Media Preparationmentioning

confidence: 99%

Antibiotic Susceptibility Pattern of Staphylococcus Aureus and Escherichia Coli Isolated from Urinary Tract Infection (UTI) Patients Attending Murtala Muhammad Specialist Hospital, Kano, Kano State, Nigeria

Umar

Sani

Muhammad³

2023

Gal Int J Health Sci Res

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The widespread use of antibiotics both inside and outside of medicine is playing a significant role in the emergence of resistant bacteria. The study was aimed to determine the antibiotic sensitivity patterns of Staphylococcus aureus and Escherichia coli isolated from Urinary Tract Infected (UTI) patients. Antibiotic susceptibility patterns of the bacterial isolates were determined using modified Kirby Bauer method. The susceptibility of antibiotic against S. aureus showed that most of the isolates were resistant to more than 3 antibiotics. Most of the isolates were sensitive to gentamicin, ofloxacin, Septrin and Augmentin. However, the isolates were more resistant to streptomycin, Nalidixic acid and levofloxacin. The susceptibility pattern of S. aureus against the antibiotics showed that 14 out of the 20 isolates were multi drug resistant. For E. coli, result showed that most of the isolates were resistant to more than 3 antibiotics. Most of the isolates were sensitive to Levofloxacin, ofloxacin, Streptomycin and gentamicin. However, the isolates were more resistant to Ceporex, Augmentin and ampicillin and 14 out of the 20 isolates were multi drug resistant. It is concluded that the bacteria isolated from UTI patients are mostly resistance to some classes of antibiotics. Keywords: Urinary Tract Infection (UTI) and antibiotics

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Section: Media Preparationmentioning

confidence: 99%

Antibiotic Susceptibility Pattern of Staphylococcus Aureus and Escherichia Coli Isolated from Urinary Tract Infection (UTI) Patients Attending Murtala Muhammad Specialist Hospital, Kano, Kano State, Nigeria

Umar

Sani

Muhammad³

2023

Gal Int J Health Sci Res

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“…Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Resistant Coagulase-Negative Staphylococci (MR-CoNS) has been identified as multidrug-resistant zoonotic pathogens in humans and many animal species [4,5]. Even through, coagulase negative Staphylococci may also be a normal flora for skin and mucous membranes of human and animal species [6].…”

Section: Introductionmentioning

confidence: 99%

Molecular Determination of Resistance Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin and Wound

S.U.,

M. D.,

U. A.

2024

JBR

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The wide spread use of antibiotic resulted in the development of resistance to antibiotics through acquisition of the mobile cassette chromosome carrying the Methicillin-resistant gene mecA. The study was aimed to characterize the resistance gene in Methicillin resistant Staphylococcus aureus (MRSA) isolated from skin and wound samples in Kano Metropolis, Northwestern, Nigeria. A total of 235 S. aureus isolates were identified and subjected to MRSA screening. MRSA were phenotypically identified by antibiotic susceptibility testing using agar disc diffusion method. The suspected MRSA were subjected to polymerase chain reaction (PCR). The PCR products were subjected to gel electrophoresis and a DNA ladder were loaded into the gel wells. The gel was examined for the presence of specific amplicons of the expected size for mecA, which is 192bp. Out of a total of 235 isolates of Staphylococcus aureus, only 11 (4.7%) strains were found to be Methicillin resistant Staphylococcus aureus (MRSA). Five out of the 11 isolates show the presence of DNA bands of the expected size for MecA gene which indicated the presence of the resistance genes in the bacterial isolates. It is concluded that MecA gene is one of the gene responsible for methicillin resistance in MRSA.

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Antimicrobial Susceptibility Pattern of Staphylococcus Species from Clinical Samples

Ali¹

2020

J Inter Med Emer Res

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The research was aimed to evaluate the antibiotic susceptibility pattern of Staphylococcus species from clinical samples obtained from some hospitals in Kano metropolis, Nigeria. The ear swab, high vaginal swab (HVS), wound swab and urine samples from the patients attending the hospitals were collected and inoculated onto the surface of freshly prepared Nutrient agar for bacterial isolation. The bacteria isolated were identified by conventional microbiological methods namely; Gram staining, biochemical test (such as catalase, coagulase, and DNase test), mannitol salt agar and heamolysis test. The isolates were subjected to antibiotic susceptibility testing using the agar disc diffusion method. The result showed that S. aureus was highly susceptible to Ciprofloxacin 105 (68.63%), Gentamicin 102 (66.67%), Levofloxacin 95 (62.08%) and Amikacin 90 (58.82%), S. epidermidis was highly susceptible to Gentamicin 13 (61.90%), Levofloxacin 12 (57.14%) and Nitrofurantoin 11 (52.38%) while S. saprophyticus was highly susceptible to Cefoxitin 7 (77.78%), Gentamicin 6 (66.67%) and Nitrofurantoin 5 (55.56%). On the other hand, S. aureus was highly resistant to Cefuroxime 153 (100%), Ceftazidime 150 (98.04%), Amoxicillin/clavulanic acid 120 (78.43%) and Cloxacillin 111 (72.55%), S. epidermidis was highly resistant to Ceftazidime 20 (95.24%), Cloxacillin 19 (90.48%) then Cefoxitin, Erythromycin and Amoxicillin/clavulanic acid with 15 (71.43%) both. S. saprophyticus was highly resistant to Cefepime 9 (100%), Cloxacillin 8 (88.89%), Ceftazidime 7 (77.78%), Imipenem and Erythromycin with 6 (66.67%) respectively. There is a statistical difference in the sensitivity of the isolates against the antibiotics used at p<0.05. It is concluded that Staphylococcus species develop resistance to some classes of antibiotics.

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Characterization and Determination of Antimicrobial Sensitivity Pattern of Staphylococcus aureus Associated with Urinary Tract Infection

Cited by 3 publications

References 5 publications

Antibiotic Susceptibility Pattern of Staphylococcus Aureus and Escherichia Coli Isolated from Urinary Tract Infection (UTI) Patients Attending Murtala Muhammad Specialist Hospital, Kano, Kano State, Nigeria

Antibiotic Susceptibility Pattern of Staphylococcus Aureus and Escherichia Coli Isolated from Urinary Tract Infection (UTI) Patients Attending Murtala Muhammad Specialist Hospital, Kano, Kano State, Nigeria

Molecular Determination of Resistance Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin and Wound

Antimicrobial Susceptibility Pattern of Staphylococcus Species from Clinical Samples

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