Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions

Jiang, Qiantao; Wei, Yuming; Wei, Feng; Wang, Jirui; Yan, Zehong; Zheng, Youliang

doi:10.1186/1471-2229-9-16

Cited by 51 publications

(59 citation statements)

References 49 publications

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“…The subunits Glu-A1y1 and Glu-A1y2 observed in this study were similar to the subunits observed by Hu et al (2011) in three species of Triticum monococcum L., Triticum boeticum Boiss and Triticum urartu Tum, while the subunit Glu-A1y3 was characterized as the study of diploid accessions of Triticum aegilopoides by Jiang et al (2009).…”

Section: Discussionsupporting

confidence: 88%

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Şan¹,

Özbek²,

Eser³

et al. 2015

Cereal Research Communications

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The objective of this study is the analysis of polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcum ssp. monococcum] landraces. The genetic diversity of high-molecular-weight (HMW) glutenin subunits and the gliadin proteins in 10 landrace populations of cultivated einkorn wheat, originating from Turkey, was investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and ammonium lactic acid polyacrylamide gel electrophoresis (A-PAGE), respectively. For glutenins, the mean number of alleles, the mean number of effective alleles, the mean value of genetic diversity and the mean value of average genetic diversity were detected as 3.50, 2.98, 0.65 and 0.28, respectively. The genetic differentiation was 0.57, while gene flow was 0.19 between populations. For gliadins, the mean number of alleles, the mean number of effective alleles, the mean value of total genetic diversity and the genetic diversity within population were detected as 2.00, 1.21, 0.17 and 0.15, respectively. The genetic differentiation was 0.08, whereas gene flow was 6.15 between populations. STRUCTURE is a software package program for population genetic analysis, was used to infer population structures of landraces populations. The optimum value for K was obtained as 10. Considering the high number of proteins and genetic variation, and increased interest in organic products, the farming of einkorn wheat should be supported and conservation of germplasm in landraces should be maintained as important genetic resources. The landraces germplasm should be conserved for future crop improvement processes.

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Section: Discussionsupporting

confidence: 88%

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Şan¹,

Özbek²,

Eser³

et al. 2015

Cereal Research Communications

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“…dicoccoides, which is the oldest species of emmer wheat with the genome AABB (Feldman et al 1995;Chantret et al 2005). The 1Ay subunit JF519636 was similar to that of AY245578 from T. urartu line IZ29-1, but differed from the other nine 1Ay genes in electrophoretic mobility in SDS-PAGE (Wan et al 2002;Bai et al 2004;Jiang et al 2009;Hu et al 2010). However, its predicted protein secondary structure was different from that of AY245578 in the relative content and number of motifs because of 25 different amino acids (Figure 3, Tables 1 and 3).…”

Section: Discussionmentioning

confidence: 99%

“…In common wheat, the gene coding the subunit 1Ay is unexpressed because of a premature stop codon (Forde et al 1985) or the insertion of a transposon-like element within its coding region (Harberd et al 1987). However, this subunit is active in many lines of diploid wheat (AA) and the tetraploid wheats T. turgidum (AABB) and T. timopheevii (AAGG) (Ciaffi et al 1991(Ciaffi et al , 1998Ma et al 2007;Jiang et al 2009;Xu et al 2009;Hu et al 2012). Active 1Ay genes have been cloned from the wild diploid wheats T. urartu and T. monococcum ssp.…”

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confidence: 99%

Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides

Bi¹,

Wu²,

Hu³

et al. 2014

Czech J. Genet. Plant Breed.

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The high molecular weight glutenin subunit (HMW-GS), encoded by the 1Ay gene, unexpressed in common wheat, exists in diploid and tetraploid wheats. An active 1Ay gene was first cloned from wild emmer wheat (T. turgidum ssp. dicoccoides, 2n = 4x = 28, AABB), the oldest species in emmer wheat. Here, a novel subunit encoded by the 1Ay gene (JF519636) present in T. turgidum ssp. dicoccoides line D141 was characterized. The protein had 608 amino acids with six cysteine residues and showed faster electrophoretic mobility than 1Dy12 from common wheat. Compared with previously reported 1Ay subunits, it contained 16 single point mutations (SPMs). Comparative and phylogenetic sequence analyses suggested that this gene was more similar to the 1Ay gene from the diploid species (2n = 2x = 14, AA) T. urartu than from T. monococcum ssp. aegilopoides. Its predicted secondary protein structure possessed a different content of motifs relative to the 1Ay gene (AY245578) from T. urartu, which had similar electrophoretic mobility. In the central repetitive domain, JF519636 had more β-turns and β-bends than the 1Ay subunit AY245578. These structural characteristics in JF519636 could possibly be associated with specific gluten properties.

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“…aegilopoides, and T. monococcum ssp. monococcum (Jiang et al 2009;Hu et al 2010;Gutiérrez et al 2011;Hu et al 2012). In this study, 1Ay subunit is expressed in 7 accessions of T. monococcum ssp.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles

Li¹,

Li²,

Zeng³

et al. 2016

Cereal Research Communications

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High-molecular-weight glutenin subunits (HMW-GSs) are important seed storage proteins associated with bread-making quality in common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD). Variation in the Glu-A1x locus in common wheat is scare. Diploid Triticum monococcum ssp. monococcum (2n = 2x = 14, A m A m ) is the first cultivated wheat. In the present study, allelic variations at the Glu-A1 m x locus were systematically investigated in 197 T. monococcum ssp. monococcum accessions. Out of the 8 detected Glu-A1 m x alleles, 5 were novel, including Glu-A1 m -b, Glu-A1 m -c, Glu-A1 m -d, Glu-A1 m -g, and Glu-A1 m -h. This diversity is higher than that of common wheat. Compared with 1Ax1 and 1Ax2*, which are present in common wheat, these alleles contained three deletions/insertions as well as some single nucleotide polymorphism variations that might affect the elastic properties of wheat flour. New variations in T. monococcum probably occurred after the divergence between A and A m and are excluded in common wheat populations. These allelic variations could be used as novel resources to further improve wheat quality.

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Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions

Cited by 51 publications

References 49 publications

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles

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Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions

Cited by 51 publications

References 49 publications

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides

Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1mxalleles

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Molecular characterization of differentTriticum monococcumssp.monococcum Glu-A1^mxalleles