Characterization and cloning of plasmids from the iron-oxidizing bacteriumThiobacillus ferrooxidans

Shiratori, Toshikazu; Inoue, Chihiro; Numata, Masahiko; Kusano, Tomonobu

doi:10.1007/bf02104133

Cited by 22 publications

(10 citation statements)

References 21 publications

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“…The habitats of A. ferrooxidans strains are geographically extremely diverse and vary in their physico-chemical conditions (presence of particular sulfide minerals and their ratio, pH, temperature and the content of toxic compounds in the liquid phase). This might explain the polymorphism of A. ferrooxidans strains, in terms of both their physiological properties (Tuovinen et al, 1971;Harrison, 1982;Valenti et al, 1989;Leduc & Ferroni, 1994;Chisholm et al, 1998;Frattini et al, 2000;Ageeva et al, 2001) and genotypic characteristics (Martin et al, 1981;Harrison, 1982;Shiratori et al, 1991;Kondratyeva et al, 1993Kondratyeva et al, , 1995Rawlings & Kusano, 1994;Rawlings, 1999). The study of the natural genotypic and phenotypic variability of bacterial strains is of great importance in discerning the microevolutionary mechanisms of species formation and for their monitoring in biohydrometallurgical processes and in nature.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Karavaiko

Turova

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Polyphasic genotypic analysis of 25 Acidithiobacillus ferrooxidans strains isolated from ores and ore concentrates collected in different regions of the world showed considerable strain heterogeneity. Restriction patterns of the chromosomal DNA of these strains obtained by PFGE were specific for each strain. According to the degree of DNA relatedness, 17 of the 23 strains studied were divided into four genomovars. Six independent, considerably divergent strains could not be assigned to any of the genomovars. A comparison of nearly complete nucleotide sequences of the 16S rDNA of five representatives of the genomovars (including the type strain of A. ferrooxidans, ATCC 23270 T ) with those of species of the genus Acidithiobacillus available from GenBank showed that most of the A. ferrooxidans strains, together with the type strain and some other strains of the species Acidithiobacillus thiooxidans, comprised a monophyletic cluster. Within this major cluster, A. ferrooxidans strains fell into four phylogenetic groups that were equidistant from the phylogenetic group of A. thiooxidans strains. In general, the distribution of strains among the phylogenetic groups correlated with their distribution among the genomovars, except that the representatives of two different genomovars fell into one phylogenetic group. Thus, at least two levels of phylogenetic heterogeneity for A. ferrooxidans have been found. The phylogenetic heterogeneity of A. ferrooxidans strains, which are phenotypically indistinguishable, suggests the occurrence of microevolutionary processes in different econiches. This should be taken into account in the biohydrometallurgical applications of A. ferrooxidans strains.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Karavaiko

Turova

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…Martin et al (86) reported that 11 of 15 strains originating mostly from the United States and Bulgaria contained one to five plasmids per strain ranging in size from 7.4 to 75 kbp. In a survey of more than 100 strains from six Japanese mining sites, 73% were found to carry one or more plasmids ranging in size from 2.0 to 30 kbp (131). Plasmids have also been reported in strains from South Africa (109), Italy and Mexico (145), Chile (124), and Canada (87).…”

Section: Natural Plasmids From T Ferrooxidansmentioning

confidence: 99%

Molecular genetics of Thiobacillus ferrooxidans.

Rawlings

Kusano

1994

Microbiological Reviews

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“…ferroidans strains (36), and pKT240 is an IncQ-type broad-host-range plasmid (1). Arrows indicate directions of transcription of the genes.…”

Section: Methodsmentioning

confidence: 99%

Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant

et al. 1992

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The mer operon from a strain of ThiobaciUus ferrooxidans (C. Inoue, K. Sugawara, and T. Kusano, Mol. Microbiol. 5:2707-2718 consists of the regulatory gene merR and an operator-promoter region followed by merC and merA structural genes and differs from other known gram-negative mer operons. We have constructed four potential shuttle plasmids composed of a T. ferrooxidans-borne cryptic plasmid, a pUC18 plasmid, and the above-mentioned mer determinant as a selectable marker. Mercury ion-sensitive T. ferro. oxidans strains were electroporated with constructed plasmids, and one strain, Y4-3 (of 30 independent strains tested), was found to have a transformation efficiency of 120 to 200 mercury-resistant colonies per ,ug of plasmid DNA. This recipient strain was confirmed to be T. ferrooxidans by physiological, morphological, and chemotaxonomical data. The transformants carried a plasmid with no physical rearrangements through 25 passages under no selective pressure. Cell extracts showed mercury ion-dependent NADPH oxidation activity.

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Characterization and cloning of plasmids from the iron-oxidizing bacteriumThiobacillus ferrooxidans

Cited by 22 publications

References 21 publications

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans

Molecular genetics of Thiobacillus ferrooxidans.

Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant

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