Characterization and chemical modification of pullulan produced from a submerged culture of Aureobasidium pullulans ATCC 15233

Hernández-Tenorio, Fabian; Giraldo-Estrada, Catalina

doi:10.1016/j.polymertesting.2022.107686

Cited by 18 publications

(7 citation statements)

References 92 publications

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“…For this reason, it can be concluded that the addition of biogenic AgNPs can change the morphology and texture of the films compared to the control. Hernandez-Tenorio and Giraldo-Estrada (2022) analyzed the surface of pullulan film produced by A. pullulans ATCC 15233 and pullulan-acetate film using SEM and showed homogeneous morphology with a compact surface structure of the former one, while small particles on the surface of the latter one were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Biopolymers are an alternative to their synthetic counterparts, characterized by biodegradability and high-performance properties ( Baranwal et al, 2022 ). Pullulan, an extracellular biodegradable polysaccharide consisting of repeating units of maltotriose attached by α-(1 → 6) linkages and produced by the polymorphic fungus Aureobasidium pullulans ( A. pullulans ), has attracted the attention of many researchers ( Rai et al, 2021 ; Gniewosz et al, 2022 ; Hernandez-Tenorio and Giraldo-Estrada, 2022 ; Roy and Rhim, 2023 ). It is produced under limiting conditions that include media composition, culture conditions (pH, temperature, and time), and the type of A. pullulans strains affecting the final fermentation efficiency ( Farris et al, 2014 ; Coltelli et al, 2020 ; Wei et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strain JTW1 for potential applications in the food industry and medicine

Wypij,

Rai,

Zemljič

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Biopolymers, such as pullulan, a natural exopolysaccharide from Aureobasidium pullulans, and their nanocomposites are commonly used in the food, pharmaceutical, and medical industries due to their unique physical and chemical properties.Methods: Pullulan was synthesized by the A. pullulans ATCC 201253 strain. Nanocomposite films based on biosynthesized pullulan were prepared and loaded with different concentrations of silver nanoparticles (AgNPs) synthesized by the Fusarium culmorum strain JTW1. AgNPs were characterized by transmission electron microscopy, Zeta potential measurements, and Fourier-transform infrared spectroscopy. In turn, the produced films were subjected to physico-chemical analyses such as goniometry, UV shielding capacity, attenuated total reflection–Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy, and their mechanical and degradation properties were assessed. The antibacterial assays of the nanoparticles and the nanocomposite films against both food-borne and reference pathogens, including Listeria monocytogenes, Salmonella infantis, Salmonella enterica, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, were performed using standard methods.Results: AgNPs were small (mean 15.1 nm), spherical, and displayed good stability, being coated with protein biomolecules. When used in higher concentrations as an additive to pullulan films, they resulted in reduced hydrophilicity and light transmission for both UV-B and UV-A lights. Moreover, the produced films exhibited a smooth surface. Therefore, it can be concluded that the addition of biogenic AgNPs did not change the morphology and texture of the films compared to the control film. The nanoparticles and nanocomposite films demonstrated remarkable antibacterial activity against both food-borne and reference bacteria. The highest activity of the prepared films was observed against L. monocytogenes.Discussion: The obtained results suggest that the novel nanocomposite films prepared from biosynthesized pullulan and AgNPs can be considered for use in the development of medical products and food packaging. Moreover, this is the first report on pullulan-based nanocomposites with mycogenic AgNPs for such applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strain JTW1 for potential applications in the food industry and medicine

Wypij,

Rai,

Zemljič

et al. 2023

Front. Bioeng. Biotechnol.

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“…The maximum value obtained (experiment 3) corresponded to an increase of more than 65% compared to the lowest value (experiment 2). Hernandez-Tenorio and Giraldo-Estrada 16 used A. pullulans ATCC 15233 size-exclusion chromatography using NaNO 3 0.1 N to prepare polymeric solutions and obtained a molecular weight of 93.57 kDa, which includes M W between 100 and 200 kDa. They showed that forming polymeric films requires obtaining pullulan with higher molecular weight, decreasing the degree of acetylation, adding other substances such as plasticizers, or mixing with other biopolymers.…”

Section: Full Factorial Designmentioning

confidence: 99%

Study of the Molecular Weight of Pullulan Produced by Aureobasidium pullulans from Industrial Waste

Oliveira,

Coelho,

Sérvulo

et al. 2023

Mat. Res.

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The objective of this research was the sustainable development of pullulan and its structural and rheological characterization. In addition, the cultivation of the A. pullulans Y2092 strain by submerged fermentation on a laboratory scale was evaluated, valuing the use of industrial waste (residual brewery's yeast), RBY, and a low-cost renewable raw material VHP (very high polarization sugar). A 2 2 factorial statistical design of experiments was used to analyze the molecular weight of the pullulans produced. The maximum value of 1955 kDa was determined for the pullulan recovered after 96 h of fermentation in the mineral medium added with 70 g/L of VHP and 50 g/L of RBY. The FTIR analysis for the pullulan obtained was similar to the commercial pullulan. The rheological behavior of pullulan solutions is shown, evidencing the relationship between this property and M w . The results from all analyses indicate that the pullulan obtained is suitable for the future production of biopolymeric films.

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“…The VOSviewer version 1.6.16 and Cor-Text Manager software types were used to determine bibliometric networks such as Co-occurrence maps, Co-authorship, and contingency matrix. It is noteworthy that the compiled documents were filtered in order to avoid the repetition of terms with abbreviations and hyphens [ 25 , 26 ].…”

Section: Bibliometric Analysis Of Deproteinized Natural Rubbermentioning

confidence: 99%

Improving Deproteinization in Colombian Latex from Hevea brasiliensis: A Bibliometric Approximation

et al. 2022

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Natural Rubber Field Latex (NRFL) allergens restrict its use in some markets due to health-threatening allergic reactions. These molecules are proteins that are related to asymptomatic sensitization and hypersensitivity mediated by immunoglobulin E (IgE). Although NRFL allergens have been investigated since the 1980s, there are still gaps in knowledge regarding the development of deproteinized natural rubber (DPNR). Therefore, in this study, the deproteinization of NRFL from the lower basin of the Cauca River, Antioquia-Colombia was evaluated using eight systems. The highest removal value was 84.4% and was obtained from the treatment containing SDS (Sodium dodecyl sulfate), Urea, and Ethanol. It was also possible to determine that at high concentrations of SDS, removal percentages higher than 70% are reached. On the other hand, all deproteinizing systems decreased NRFL Zeta potentials without self-coagulation, suggesting enhanced colloidal stability in DPNR latex. On the other hand, the bibliometric analysis presented technological advances in DPRN through different parameters and bibliometric networks. The analysis presented makes an important contribution from the bibliometric approach that could be positive for the development of research on DPNR.

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Characterization and chemical modification of pullulan produced from a submerged culture of Aureobasidium pullulans ATCC 15233

Cited by 18 publications

References 92 publications

Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strain JTW1 for potential applications in the food industry and medicine

Pullulan-based films impregnated with silver nanoparticles from the Fusarium culmorum strain JTW1 for potential applications in the food industry and medicine

Study of the Molecular Weight of Pullulan Produced by Aureobasidium pullulans from Industrial Waste

Improving Deproteinization in Colombian Latex from Hevea brasiliensis: A Bibliometric Approximation

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