Characterization and biocontrol measures of Pseudomonas syringae pv. syringae associated with citrus blast disease

Islam, Md. Samiul; Sultana, Razia; Hasan, Md. Amit; Alam, Md. Shah; Sikdar, Biswanath; Kamaruzzaman,

doi:10.1007/s42535-020-00138-1

Cited by 11 publications

(7 citation statements)

References 58 publications

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“…syringae, reported for commercial essential oils by Shabani et al [75], cannot be directly compared, since the essential oils were utilized as biofumigants. The same holds true for the findings of Islam et al [76], where a different methodology and different units were employed.…”

Section: Comparison Of Efficacy With Other Natural Compoundssupporting

confidence: 63%

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Sánchez-Hernández

García

Casanova-Gascón

et al. 2022

Plants

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Cork, an anatomic adaptation of the bark of Quercus suber L. through its suberization process, finds its main application in the production of bottle stoppers. Its processing results in a large waste stream of cork fragments, granulates, and dust, which may be susceptible to valorization. The work presented here explored the use of its extracts to inhibit the growth of phytopathogenic microorganisms associated with apple tree diseases. The in vitro antimicrobial activity of cork aqueous ammonia extract was assayed against four fungi, viz. Monilinia fructigena and M. laxa (brown rot), Neofussicoccum parvum (dieback), and Phytophthora cactorum (collar and root rot), and two bacteria, viz. Erwinia amylovora and Pseudomonas syringae pv. syringae, either alone or in combination with chitosan oligomers (COS). Effective concentration values of EC90 in the 675–3450 μg·mL−1 range, depending on the fungal pathogen, were obtained in growth inhibition tests, which were substantially improved for the conjugate complexes (340–801 μg·mL−1) as a result of strong synergism with COS. Similar enhanced behavior was also observed in antibacterial activity assays, with MIC values of 375 and 750 μg·mL−1 for the conjugate complexes against P. syringae pv. syringae and E. amylovora, respectively. This in vitro inhibitory activity was substantially higher than those exhibited by azoxystrobin and fosetyl-Al, which were tested for comparison purposes, and stood out among those reported for other natural compounds in the literature. The observed antimicrobial activity may be mainly attributed to the presence of glycerin and vanillic acid, identified by gas chromatography–mass spectroscopy. In the first step towards in-field application, the COS–Q. suber bark extract conjugate complex was further tested ex situ against P. cactorum on artificially inoculated excised stems of the ‘Garnem’ almond rootstock, achieving high protection at a dose of 3750 μg·mL−1. These results suggest that cork industrial leftovers may, thus, be a promising source of bioactive compounds for integrated pest management.

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Section: Comparison Of Efficacy With Other Natural Compoundssupporting

confidence: 63%

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Sánchez-Hernández

García

Casanova-Gascón

et al. 2022

Plants

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“…All the sequences were aligned by the Neighbor-Joining method through the MEGA 11.0 multiple sequence alignment software [ 71 ]. The pairwise alignment parameters were considered as gap opening penalty 16, extension penalty 6.67, delay cutoff 35%, and transition weight of DNA 35%; weight matrix IUB with 1000 bootstrap replicates [ 72 ]. Nucleotide missing or fragmented data for every gene were erased.…”

Section: Methodsmentioning

confidence: 99%

Identification of host transcriptome-guided repurposable drugs for SARS-CoV-1 infections and their validation with SARS-CoV-2 infections by using the integrated bioinformatics approaches

Ahmed

Reza

Sarker³

et al. 2022

PLoS ONE

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Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is one of the most severe global pandemic due to its high pathogenicity and death rate starting from the end of 2019. Though there are some vaccines available against SAER-CoV-2 infections, we are worried about their effectiveness, due to its unstable sequence patterns. Therefore, beside vaccines, globally effective supporting drugs are also required for the treatment against SARS-CoV-2 infection. To explore commonly effective repurposable drugs for the treatment against different variants of coronavirus infections, in this article, an attempt was made to explore host genomic biomarkers guided repurposable drugs for SARS-CoV-1 infections and their validation with SARS-CoV-2 infections by using the integrated bioinformatics approaches. At first, we identified 138 differentially expressed genes (DEGs) between SARS-CoV-1 infected and control samples by analyzing high throughput gene-expression profiles to select drug target key receptors. Then we identified top-ranked 11 key DEGs (SMAD4, GSK3B, SIRT1, ATM, RIPK1, PRKACB, MED17, CCT2, BIRC3, ETS1 and TXN) as hub genes (HubGs) by protein-protein interaction (PPI) network analysis of DEGs highlighting their functions, pathways, regulators and linkage with other disease risks that may influence SARS-CoV-1 infections. The DEGs-set enrichment analysis significantly detected some crucial biological processes (immune response, regulation of angiogenesis, apoptotic process, cytokine production and programmed cell death, response to hypoxia and oxidative stress), molecular functions (transcription factor binding and oxidoreductase activity) and pathways (transcriptional mis-regulation in cancer, pathways in cancer, chemokine signaling pathway) that are associated with SARS-CoV-1 infections as well as SARS-CoV-2 infections by involving HubGs. The gene regulatory network (GRN) analysis detected some transcription factors (FOXC1, GATA2, YY1, FOXL1, TP53 and SRF) and micro-RNAs (hsa-mir-92a-3p, hsa-mir-155-5p, hsa-mir-106b-5p, hsa-mir-34a-5p and hsa-mir-19b-3p) as the key transcriptional and post- transcriptional regulators of HubGs, respectively. We also detected some chemicals (Valproic Acid, Cyclosporine, Copper Sulfate and arsenic trioxide) that may regulates HubGs. The disease-HubGs interaction analysis showed that our predicted HubGs are also associated with several other diseases including different types of lung diseases. Then we considered 11 HubGs mediated proteins and their regulatory 6 key TFs proteins as the drug target proteins (receptors) and performed their docking analysis with the SARS-CoV-2 3CL protease-guided top listed 90 anti-viral drugs out of 3410. We found Rapamycin, Tacrolimus, Torin-2, Radotinib, Danoprevir, Ivermectin and Daclatasvir as the top-ranked 7 candidate-drugs with respect to our proposed target proteins for the treatment against SARS-CoV-1 infections. Then, we validated these 7 candidate-drugs against the already published top-ranked 11 target proteins associated with SARS-CoV-2 infections by molecular docking simulation and found their significant binding affinity scores with our proposed candidate-drugs. Finally, we validated all of our findings by the literature review. Therefore, the proposed candidate-drugs might play a vital role for the treatment against different variants of SARS-CoV-2 infections with comorbidities, since the proposed HubGs are also associated with several comorbidities.

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“…DNA samples were stored at −20°C for PCR analysis. The 16S rRNA and gyrB gene regions were ampli ed with the primer pairs of 27-F/1391-R and gyrB-F/ gyrB-R, respectively (Grifoni et al, 1995;Ivanović et al, 2017;Islam et al, 2020). PCR ampli cation was performed in a Hybaid Omnigen thermocycler (Teddington, Middlesex, UK).…”

Section: Dna Extraction Pcr Ampli Cation and Sequence Analysismentioning

confidence: 99%

Pseudomonas syringae pv. coronafaciens, causing a new bacterial halo blight disease on oat (Avena sativa L.) in China

Wang,

Zhang,

Chen

et al. 2024

Preprint

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Pseudomonas syringae pv. coronafaciens (Ps-c) is a pathogenic bacterium species that can be found in agricultural environments that causes diseases in plants. During a survey in 2019–2020 of different areas, bacterial halo blight (BHB) caused by Ps-c in oat (Avena sativa L.) was observed in many oat fields in Huan county, Gansu Province of China. Average leaf disease incidence was approximately 1 to 14%. The symptoms of BHB were mainly observed on leaves of oat and appeared as white to yellow streaks. After 20–30 d, the streaks became yellow necrotic lesions and spread to whole leaves, eventually causing leaves to wilt and decline. Symptomatic leaves of affected oats were collected from locations to investigate the etiology of BHB disease, and a group of 32 bacterial isolates (HK7) phenotypically similar was consistently isolated from the necrotic lesion margins in all samples. Pathogenicity tests of this group isolates by wounding and injection inoculations in greenhouse demonstrated that the symptoms induced by them on oat were similar to those observed in the field. HK7 isolates were identified as Ps-c based on Biolog Gen III MicroStation, morphological, physiological and biochemical characterization, 16S rRNA and gyrB gene sequence analysis, which compared with a reference strain (Ps-c ATCC 19608). Furthermore, Ps-c had a narrow host range and was host specific. After inoculation of Ps-c on A. sativa (B7) and A. nuda (B2) in greenhouse, effective tillers, plant height, flag-leaf length, flag-leaf width, fresh weight, dry weight, panicle length, grains per spike, reproductive branches number and thousand grains weight of infected oat were averagely decreased by 19.44%, 11.49%, 11.34%, 9.23%, 11.61%, 7.57%, 6.11%, 9.73%, 9.31% and 4.15% compared with that of healthy oat, respectively. Therefore, BHB (Ps-c) is a new bacterial disease on oat in China and has negative influences on plant growth, forage and grain yield of oat, and these negative effects are enhanced with the increase of disease severity.

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Characterization and biocontrol measures of Pseudomonas syringae pv. syringae associated with citrus blast disease

Cited by 11 publications

References 58 publications

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Identification of host transcriptome-guided repurposable drugs for SARS-CoV-1 infections and their validation with SARS-CoV-2 infections by using the integrated bioinformatics approaches

Pseudomonas syringae pv. coronafaciens, causing a new bacterial halo blight disease on oat (Avena sativa L.) in China

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