Characterization and biocompatibility of bacterial cellulose/alginate composite sponges with human keratinocytes and gingival fibroblasts

Chiaoprakobkij, Nadda; Sanchavanakit, Neeracha; Subbalekha, Keskanya; Pavasant, Prasit; Phisalaphong, Muenduen

doi:10.1016/j.carbpol.2011.03.011

Cited by 137 publications

(74 citation statements)

References 18 publications

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“…The current FDA limits for endotoxin contamination is <20,0 EU/ medical device (USP, 2011b), or 5 EU/kg of body weight for parenteral drugs (USP, 2011a). Endotoxins can be destroyed using high temperatures (>250 ºC for more than 30 min), or alkalis or acids of at least 0,1 M strength (Gorbet & Sefton, 2005;Magalhães, Lopes, Mazzola, Rangel-Yagui, Penna & Pessoa Jr, 2007), with NaOH commonly used for purification of bacterial cellulose (Bodin, Bharadwaj, Wu, Gatenholm, Atala & Zhang, 2010;Cherian et al, 2013;Chiaoprakobkij, Sanchavanakit, Subbalekha, Pavasant & Phisalaphong, 2011;Helenius, Bäckdahl, Bodin, Nannmark, Gatenholm & Risberg, 2006;Maneerung, Tokura & Rujiravanit, 2008;Saska et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

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 Cellulose nanofibril dispersion (50 µg/ml) did not affect the cytotoxicity or metabolic activity of Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes  Aerogels made of cellulose nanofibrils induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death  Cytokine profiling measuring 27 cytokines revealed that the keratinocytes and fibroblasts did not induce cytokines upon direct exposure to the CNF materials. Due to the nano dimension of the CNFs, the aerogels had a high moisture-holding capacity (~7500%) 3 AbstractWood cellulose nanofibrils have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide followed by TEMPO mediated oxidation was developed to produce ultrapure cellulose nanofibrils (CNF), with an endotoxin level of 45 endotoxin units/g (EU/g) cellulose. Scanning transmission electron microscopy (S(T)EM) revealed a highly nanofibrillated structure (lateral width of 3.7±1.3 nm).Assessment of cytotoxicity and metabolic activity on Normal Human Dermal Fibroblasts and Human Epidermal Keratinocytes was done. CNF-dispersion of 50 g/ml did not affect the cells. CNF-aerogels induced a reduction of metabolic activity by the fibroblasts and keratinocytes, but no significant cell death. Cytokine profiling revealed no induction of the 27 cytokines tested upon exposure to CNF. The moisture-holding capacity of aerogels was relatively high (~7500%), compared to a commercially available wound dressing (~2500%),indicating that the CNF material is promising as dressing material for management of wounds with a moderate to high amount of exudate.

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Section: Introductionmentioning

confidence: 99%

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Nordli

Chinga-Carrasco²,

Rokstad

et al. 2016

Carbohydrate Polymers

125

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“…However, its dense nanocellulose network prevents cells from penetrating the material. To circumvent this problem, several techniques have been developed to support cell ingrowth in BNC scaffolds by tuning pore size and pore interconnectivity during biosynthesis of BNC [35], via laser ablation [10] and freeze-dry processing [36,37]. Such macroporous BNC scaffolds have been shown to provide an adequate environment that supports ingrowth and differentiation of chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

et al. 2015

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“…These can be improved by preparation of three-dimensional networks structure of hydrogels via physical, chemical and ionic crosslinking [3]. Alginate, a linear polysaccharide (1-4) linked Lguluronic and D-mannuronic acid extracted from brown seaweed, can be ionic cross-linked by divalent or trivalent ions of both guluronic acid and mannuronic acid groups in the side chain leading to water-insoluble [4] Carboxymethyl cellulose (CMC), a hydrophilic anionic polymer derived from cellulose, enable water binding and moisture sorption properties due to its many hydroxyl and carboxylic groups. It can be chemical cross-linked using bifunctional crosslinking agent such as epichrolohydrin, formaldehyde and can be then swelled to form hydrogels and absorbs large amount of water [5,6].…”

Section: Introductionmentioning

confidence: 99%

Alginate/Carboxymethyl Cellulose Hydrogel Films in Relation to Crosslinking with Glutaraldehyde and Copper Sulfate

Sritweesinsub

Charuchinda

2015

MATEC Web of Conferences

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Abstract. Alginate/carboxymethyl cellulose (CMC) hydrogel films using glutaraldehyde (GA) and/or copper sulfate (Cu 2+ ) as crosslinking agents were prepared. The ratios of alginate to CMC were varied at 0:10, 2:8, 4:6, 6:4, 8:2 and 10:0 in the presence of GA at 0, 2, 5% and/or copper sulfate of 0 and 0.02 mol. The Fourier Transform Infrared Spectroscopy (FTIR) spectra of hydrogels indicated the intermolecular interaction between CMC with GA and alginate with Cu 2+ . Using a couple of GA and Cu 2+ , as crosslinking agents, the hydrogels exhibited better swelling time than that of a crosslinking agent alone. The percentages of solid remains were increased by increasing both of alginate ratio and Cu 2+ content.

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Characterization and biocompatibility of bacterial cellulose/alginate composite sponges with human keratinocytes and gingival fibroblasts

Cited by 137 publications

References 18 publications

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells

Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

Alginate/Carboxymethyl Cellulose Hydrogel Films in Relation to Crosslinking with Glutaraldehyde and Copper Sulfate

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