Characterization and application of a novel Aeromonas bacteriophage as treatment for pathogenic Aeromonas hydrophila infection in rainbow trout

Cao, Yanghui; Li, Shaowu; Han, Shicheng; Wang, Di; Zhao, Juanjuan; Xu, Liming; Liu, Hongbai; Lü, Tao

doi:10.1016/j.aquaculture.2020.735193

Cited by 34 publications

(40 citation statements)

References 31 publications

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“…But significantly different from that of mammals( Bos taurus [ 33 ] and human [ 34 ], etc), this result may be due to bighead carp belonging to the chordata phylum. Proteobacteria are widely distributed in the environment, Aeromonas belongs to Proteobacteria , and most members of Aeromonas are potential pathogens in aquaculture, like Aeromonas veronii [ 35 ], Aeromonas hydrophila [ 36 ], When the conditions are appropriate, the bacteria can cause fish disease. This result is consistent with an earlier study suggesting that gut may be a reservoir for many conditional pathogens [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of gut microbial communities, free amino acids or fatty acids contents in the muscle of wild Aristichthys nobilis from Xinlicheng reservoir and Chagan lake

Zhang

et al. 2022

BMC Microbiol

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Background Fish is favored by consumers, while amino acids and fatty acids are the main nutrients of muscle. At present, it has been found that the gut microbial community may be involved in the regulation of host material anabolism. Juvenile and adult bighead carp (A. nobilis) from Chagan lake and Xinlicheng reservoir were selected, and divided into four groups to compare the differences of gut microbial communities, free amino acid and fatty acids in muscle. Results The results showed that fish in different lakes or ages contained specific microbiota, the gut microbial structure was similar, but the microbial content was significantly different. Gut microbial abundance of juvenile fish in Chagan lake was significantly higher than that of other groups. Phylum level analysis Proteobacteria was the dominant gut bacteria of fish in both adult and juvenile fish from two separate lakes. Actinobacteria was another dominant bacterial phylum in juvenile fish in both lakes. Contents of free amino acids and fatty acids in muscle were detected, and the relationships between them and gut microbial communities were analyzed. Bighead carp grew from juvenile to adult, Actinobacteria abundance decreased (P < 0.05) and Proteobacteria increased (P < 0.05). Proteobacteria was positively correlated with the contents of Thr, Lys, Pro, Asp, Gly and Glu, Actinobacteria was negatively correlated with Met and His. Meanwhile, EPA and DHA were positively correlated with Proteobacteria, EPA and DHA were not significantly associated with Actinobacteria. Conclusion It was speculated that the contents of free amino acids and fatty acids in muscle might be affected by the difference of gut microbiota, thus affecting the taste and nutritional quality.

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Section: Discussionmentioning

confidence: 99%

Comparison of gut microbial communities, free amino acids or fatty acids contents in the muscle of wild Aristichthys nobilis from Xinlicheng reservoir and Chagan lake

Zhang

et al. 2022

BMC Microbiol

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“…Extracellular products: (e.g., hemolysin, protease, lipase, protease, DNases cytotoxic enterotoxin) Secretion systems: Type II secretion system Type III secretion system Type IV secretion system Type VI secretion system [54, 85,86] A. hydrophila is an opportunistic pathogen with a wide host range (e.g., amphibians, birds, fish, reptiles and mammals) [87,88] and is responsible for several bacterial diseases that have caused the loss of millions of dollars in the global freshwater aquaculture industry [6,63,89]. A. hydrophila can infect several freshwater and marine fish, causing "Motile Aeromonas Septicemia (MAS)", or "Red-Sore Disease" [90], which results in lesions, scale shedding, gill and anal hemorrhage, abdominal swelling, skin ulcers and septicemia [91]. Different fish species, including tilapia (Oreochromis niloticus), catfish (Ictalurus punctatus), striped catfish (Pangasianodon hypophthalmus), goldfish (C. auratus), common carp (C. carpio) and eel (Anguilla spp.)…”

Section: Optimum Growth Phmentioning

confidence: 99%

Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

et al. 2022

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Aeromonas species often cause disease in farmed fish and are responsible for causing significant economic losses worldwide. Although vaccination is the ideal method to prevent infectious diseases, there are still very few vaccines commercially available in the aquaculture field. Currently, aquaculture production relies heavily on antibiotics, contributing to the global issue of the emergence of antimicrobial-resistant bacteria and resistance genes. Therefore, it is essential to develop effective alternatives to antibiotics to reduce their use in aquaculture systems. Bacteriophage (or phage) therapy is a promising approach to control pathogenic bacteria in farmed fish that requires a heavy understanding of certain factors such as the selection of phages, the multiplicity of infection that produces the best bacterial inactivation, bacterial resistance, safety, the host’s immune response, administration route, phage stability and influence. This review focuses on the need to advance phage therapy research in aquaculture, its efficiency as an antimicrobial strategy and the critical aspects to successfully apply this therapy to control Aeromonas infection in fish.

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“…A. hydrophila can infect a wide variety of freshwater and marine fish, causing motile Aeromonas septicemia (MAS) [ 2 ]. The symptoms of MAS include swelling of tissues, dropsy, red sores, necrosis, ulceration and hemorrhagic septicemia [ 3 ]. MAS affects different fish species, including tilapia ( Oreochromis niloticus ), catfish ( Ictalrus punctatus ), goldfish ( Carassius auratus ), common carp ( Cyprinus carpio L.) and eel ( Anguilla spp.…”

Section: Introductionmentioning

confidence: 99%

Construction and Characterization of an Aeromonas hydrophila Multi-Gene Deletion Strain and Evaluation of Its Potential as a Live-Attenuated Vaccine in Grass Carp

et al. 2021

Vaccines

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Aeromonas hydrophila is an important pathogen that causes motile Aeromonas septicemia (MAS) in the aquaculture industry. Aerolysin, hemolysin, serine protease and enterotoxins are considered to be the major virulence factors of A. hydrophila. In this study, we constructed a five-gene (aerA, hly, ahp, alt and ast) deletion mutant strain (named Aeromonas hydrophila five-gene deletion strain, AHFGDS) to observe the biological characteristics and detect its potential as a live-attenuated vaccine candidate. AHFGDS displayed highly attenuated and showed increased susceptibility to fish blood and skin mucus killing, while the wild-type strain ZYAH72 was highly virulent. In zebrafish (Danio rerio), AHFGDS showed a 240-fold higher 50% lethal dose (LD50) than that of the wild-type strain. Immunization with AHFGDS by intracelomic injection or immersion routes both provided grass carp (Ctenopharyngodon idella) significant protection against the challenge of the strain ZYAH72 or J-1 and protected the fish organs from serious injury. Further agglutinating antibody titer test supported that AHFGDS could elicit a host-adaptive immune response. These results suggested the potential of AHFGDS to serve as a live-attenuated vaccine to control A. hydrophila infection in aquaculture.

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Characterization and application of a novel Aeromonas bacteriophage as treatment for pathogenic Aeromonas hydrophila infection in rainbow trout

Cited by 34 publications

References 31 publications

Comparison of gut microbial communities, free amino acids or fatty acids contents in the muscle of wild Aristichthys nobilis from Xinlicheng reservoir and Chagan lake

Comparison of gut microbial communities, free amino acids or fatty acids contents in the muscle of wild Aristichthys nobilis from Xinlicheng reservoir and Chagan lake

Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View

Construction and Characterization of an Aeromonas hydrophila Multi-Gene Deletion Strain and Evaluation of Its Potential as a Live-Attenuated Vaccine in Grass Carp

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