Characterization and antioxidant activities of extracellular and intracellular polysaccharides from Fomitopsis pinicola

Hao, Limin; Sheng, Zhicun; Lu, Jike; Tao, Ruyu; Jia, Shiru

doi:10.1016/j.carbpol.2015.11.048

Cited by 88 publications

(44 citation statements)

References 18 publications

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“…The chemical structures of peak fractions isolated by HSCCC were identified according to their melting points, UV, FTIR, and 1 H and 13 C NMR spectroscopy. The data of peak 1 and peak 2 in Figure were consistent with those described previously (Hao et al, ; Lyu et al, ; Sun & Yang, ). The peak fractions were identified as liquiritigenin (peak 1) and glycyrrhetic acid (peak 2), respectively.…”

Section: Resultssupporting

confidence: 91%

“…The absorbance A 2 was determined with a mixture of 2 mL absolute ethanol solution and 2 mL DPPH solution. The scavenging rate was calculated according to the following formula:

{SE}_{DPPH} (() %) = ([], 1 ‐ \frac{A_{1} - A_{2}}{A {}_{0}}) \times 100

Referring to the Fenton reaction system, the samples of liquiritigenin, glycyrrhetic acid, and VC were prepared with a solution concentration of 10, 20, 40, 60, and 80 μg/mL, while ethanol was used as the solvent (Hao, Sheng, & Lu, ). About 1.0 mL of sample was added to each test tube.…”

Section: Methodsmentioning

confidence: 99%

“…Referring to the Fenton reaction system, the samples of liquiritigenin, glycyrrhetic acid, and VC were prepared with a solution concentration of 10, 20, 40, 60, and 80 μg/mL, while ethanol was used as the solvent (Hao, Sheng, & Lu, 2016). About 1.0 mL of sample was added to each test tube.…”

Section: Antioxidant Activity Assaymentioning

confidence: 99%

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Preparative separation of liquiritigenin and glycyrrhetic acid from Glycyrrhiza uralensis Fisch using hydrolytic extraction combined with high‐speed countercurrent chromatography

Wang

Huang

Lü

2020

Biomedical Chromatography

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The objective of this paper was to develop a preparative method for the isolation and purification of liquiritigenin and glycyrrhetic acid from Glycyrrhiza uralensis Fisch using hydrolytic extraction combined with high‐speed countercurrent chromatography (HSCCC). Liquiritigenin and glycyrrhetic acid were well hydrolyzed from liquiritin and glycyrrhizic acid by hydrochloric acid, respectively. The optimal extraction conditions were obtained by single‐factor and orthogonal experiments, which were 100% ethanol, 1.5 mol/L hydrochloric acid, 1:25 ratio of solid to liquid, and extracted 2 h for one time. Using the two‐phase solvent system of n‐hexane‐ethyl acetate‐methanol–water (4:5:4:5, v/v), 2.1 mg liquiritigenin (the purity was 96.5% with a recovery of 87.6%) and 12.3 mg glycyrrhetic acid (the purity was 97.1% with a recovery of 74.4%) were obtained from 315‐mg crude extraction by HSCCC. The retention ratio of stationary phase was 47.2%. Their structures were identified by HPLC, melting points, UV, Fourier‐transform infrared, Electrospray ionization‐MS, 1H nuclear magnetic resonance (NMR), and 13C NMR spectra. According to the antioxidant activity assays, liquiritigenin and glycyrrhetic acid had some scavenging abilities on 1,1‐diphenyl‐2‐picrylhydrazyl free radicals; liquiritigenin had stronger scavenging ability on hydroxyl radicals.

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Section: Resultssupporting

confidence: 91%

“…The absorbance A 2 was determined with a mixture of 2 mL absolute ethanol solution and 2 mL DPPH solution. The scavenging rate was calculated according to the following formula:

{SE}_{DPPH} (() %) = ([], 1 ‐ \frac{A_{1} - A_{2}}{A {}_{0}}) \times 100

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Preparative separation of liquiritigenin and glycyrrhetic acid from Glycyrrhiza uralensis Fisch using hydrolytic extraction combined with high‐speed countercurrent chromatography

Wang

Huang

Lü

2020

Biomedical Chromatography

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“…Furthermore, the protein and nucleic acid contents in the polysaccharide fractions were assessed using ultraviolet spectrometry (180–640 nm wavelengths). Each fraction had only one main peak, and the main peaks were collected, dialyzed, desalted, concentrated, and lyophilized for use in subsequent assays [22]. …”

Section: Methodsmentioning

confidence: 99%

Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis

et al. 2017

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Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine alga Gracilariopsis lemaneiformis (PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antitumor activity. We demonstrated that PGL exerted its antitumor activities by modulating cell viability, morphology, apoptosis, and the apoptosis-related Fas/FasL signaling pathway in the human lung cancer cell line A549, the gastric cancer cell line MKN28, and the mouse melanoma cell line B16. Our data provide the first evidence that PGL inhibits cell proliferation by inducing apoptosis, which is largely mediated by Fas/FasL in cancer cells, suggesting that PGL might be a novel therapeutic agent against cancer.

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“…Both in the biofilm and planktonic microenvironment, EPS functions as a biopolymer barrier for the community and can be an alternative energy resource under nutrient-depleted conditions (Zhang and Bishop, 2003). For instance, Fomitopsis pinicola produces EPS to scavenge hydroxyl radicals and to block UV damage, thus maintaining the viability and activity of the surrounding microorganisms (Hao et al, 2016). EPS can also help partition an anaerobic microenvironment by preventing contact between anoxic species and oxygen (Elliott et al, 2014), further demonstrating the applicability of EPS for artificial consortia.…”

Section: Interkingdom Consortia Have Enhanced Tolerance Towards Extermentioning

confidence: 99%

Interkingdom microbial consortia mechanisms to guide biotechnological applications

Shu

Merino

Okamoto

et al. 2018

Microbial Biotechnology

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SummaryMicrobial consortia are capable of surviving diverse conditions through the formation of synergistic population‐level structures, such as stromatolites, microbial mats and biofilms. Biotechnological applications are poised to capitalize on these unique interactions. However, current artificial co‐cultures constructed for societal benefits, including biosynthesis, agriculture and bioremediation, face many challenges to perform as well as natural consortia. Interkingdom microbial consortia tend to be more robust and have higher productivity compared with monocultures and intrakingdom consortia, but the control and design of these diverse artificial consortia have received limited attention. Further, feasible research techniques and instrumentation for comprehensive mechanistic insights have only recently been established for interkingdom microbial communities. Here, we review these recent advances in technology and our current understanding of microbial interaction mechanisms involved in sustaining or developing interkingdom consortia for biotechnological applications. Some of the interactions among members from different kingdoms follow similar mechanisms observed for intrakingdom microbial consortia. However, unique interactions in interkingdom consortia, including endosymbiosis or interkingdom‐specific cell–cell interactions, provide improved mitigation to external stresses and inhibitory compounds. Furthermore, antagonistic interactions among interkingdom species can promote fitness, diversification and adaptation, along with the production of beneficial metabolites and enzymes for society. Lastly, we shed light on future research directions to develop study methods at the level of metabolites, genes and meta‐omics. These potential research methods could lead to the control and utilization of highly diverse microbial communities.

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Characterization and antioxidant activities of extracellular and intracellular polysaccharides from Fomitopsis pinicola

Cited by 88 publications

References 18 publications

Preparative separation of liquiritigenin and glycyrrhetic acid from Glycyrrhiza uralensis Fisch using hydrolytic extraction combined with high‐speed countercurrent chromatography

Preparative separation of liquiritigenin and glycyrrhetic acid from Glycyrrhiza uralensis Fisch using hydrolytic extraction combined with high‐speed countercurrent chromatography

Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis

Interkingdom microbial consortia mechanisms to guide biotechnological applications

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