Characterization and antimicrobial susceptibility pattern of clinical isolates of Enterococci at a tertiary care hospital in Mumbai, India

Shinde, Rupali; Koppikar, Geeta; Oommen, Seema

doi:10.4103/1755-6783.95956

Cited by 14 publications

(11 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Majority of Enterococcal infections were detected from hospitalized patients and more commonly seen among patients in intensive care units (ICUs) and surgical wards. Similar incidence rate were found by other authors [25]. Prolonged ICU hospital stay and increased invasive procedure applications are some possible explanations.…”

Section: Discussionsupporting

confidence: 87%

“…Present study revealed the most frequent infection was urinary tract infections (58.6%), followed by skin and soft tissue infections (31.6%) and blood stream infections (BSIs). Similar distribution of Enterococcal infections were reported in previous studies [22,24,25]. In these settings, enterococci are part of endogenous mixed flora commonly found in the gastrointestinal tract [26].…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Sahu

Dash

Paty

et al. 2015

Afro-Egyptian Journal of Infectious and Endemic Diseases

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 84%

Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Sahu

Dash

Paty

et al. 2015

Afro-Egyptian Journal of Infectious and Endemic Diseases

View full text Add to dashboard Cite

show abstract

“…A similar rising trend of enterococcal resistance to various antibiotics was noted in another Indian study by Shinde et al in a tertiary care hospital in Mumbai. 24 Similar increasing resistance to quinolones and tetracycline group of drugs shown by Ali S et al 25 Study has revealed very encouraging results for nitrofurantoin and linezolid, as 94.9% and 97.81% of enterococcal isolates were susceptible to them respectively. Another antimicrobial which has given increasingly encouraging results is that all our isolates remained susceptible to fosfomycin (100%) and tigecycline (100%).…”

Section: Discussionmentioning

confidence: 55%

Recent pattern of drug sensitivity of most commonly isolated uropathogens from Central India

Lawhale¹,

Naikwade

2017

Int J Res Med Sci

View full text Add to dashboard Cite

INTRODUCTIONUrinary tract infection (UTI) is one of the most common bacterial infection in the human population affecting all the age groups.1 It remains a major public health problem in terms of morbidity and financial cost. Worldwide about 150 million people are being diagnosed with urinary tract infection every year.2 Studies suggest that the overall prevalence of UTI is higher in women, an estimated 50% of women experience at least one episode of UTI at some point of their lifetime and between 20% and 40% of women can have recurrent episodes.3,4 UTI is less common in males as compared to females. 4 Urinary tract infection is due to an inflammatory response of urothelium to the invading pathogenic organisms.2 E-coli is the most common bacteria associated with the etiology of UTI. Other organisms ABSTRACT Background:The objective of the study is to assess the latest antibiotic sensitivity pattern of most commonly isolated organisms in urinary tract infection (UTI). Widespread and irrational use of antibiotics has led to development of highly resistant microorganisms. As the antibiotic sensitivity patterns of the microorganisms are frequently changing, this study was performed to assess the recent antibiotic sensitivity pattern in urinary tract infection among the human population. Methods: The study conducted from July 2016 to April 2017, in clinical microbiology laboratory, Sara Diagnostics (fully automated) laboratories, Nagpur, Maharashtra, India. 3000 urine samples were studied. The processing of the samples were done by standard microbiological methods. The antibiotic susceptibility was measured by disk diffusion test. CLSI guidelines were used for the antibiotic susceptibility evaluation. Results: Out of these, 1729 (57.63%) samples were culture positive showing significant bacteriuria. The most common bacteria isolated was E. coli (n=1040, 60.15%), followed by Enterococcus spp. (n=275, 15.90%), Klebsiella pneumoniae (n=210, 12.14%), Staphylococcus aureus (n=105, 6.07%) and Pseudomonas spp. (n=50, 2.89%) in this study. The study showed marked resistance to doxycycline, quinolones, cephalosporins and greater sensitivity to nitrofurantoin and fosfomycin, showing them better treatment options. Conclusions: The study confirmed, E.coli is still the most common bacteria to cause UTI, irrespective of geographical area. Nitrofurantoin, fosfomycin showed very high susceptibility to urinary tract infection pathogens, which include most commonly isolated gram-negative bacteria and gram-positive cocci, which were resistant to other various antibiotic groups. Thus, nitrofurantoin and fosfomycin seemed to be promising drugs.

show abstract

“…3,11,12 Maximum number of isolates was from urine which correlates with other studies. 3,12,13 38.2 % and 44.7 % of E. faecalis isolates were resistant to ampicillin and penicillin respectively in our study (Table-1) correlating with the study done by Shrihari et al 3 Amongst E. faecium isolates, 46.2 % were resistant to ampicillin and 53.8 % were resistant to penicillin (Table-2) correlating with study done by Golia et al 14 High level gentamicin resistance accounted to 22.4 % and 30.8 % for E. faecalis and E. faecium respectively by Kirby Bauer disc diffusion method (Table 1&2). Higher resistance to gentamicin leads to the failure of synergistic combination therapy with gentamicin and beta-lactam antibiotics/glycopeptides for serious infections.…”

Section: Discussionmentioning

confidence: 99%

Solation, Identification and Speciation of Enterococci and Their Antimicrobial Susceptibility in a Tertiary Care Hospital

Haritsa¹,

N²,

Chavan³

et al. 2014

jemds

View full text Add to dashboard Cite

BACKGROUND:Enterococci have emerged as the second most common cause of nosocomial infections over the past 2-3 decades and antibiotic resistance is a major obstacle for treatment. Identification of enterococci to species level is helpful and crucial for proper patient treatment, epidemiologic and infection control purposes. The emergence of vancomycin resistant enterococci is of particular concern as it has limited the therapeutic options available for the clinicians. AIM: To speculate and determine the antimicrobial susceptibility of clinical isolates of enterococci with special reference to vancomycin. SETTINGS AND DESIGN: A prospective descriptive study was carried out in Rajarajeswari medical college and hospital, Bangalore. MATERIAL AND METHODS: The study included a total of 90 clinical isolates of enterococcus from urine, pus, blood and endotracheal aspirates over a period of one year from November 2012 to October 2013. The isolates were confirmed to belong to genus Enterococcus and speciation was based on potassium tellurite (0.04 %) reduction, arginine deamination and fermentation of arabinose, mannitol, raffinose, and sorbitol. Antimicrobial susceptibility was determined by Kirby Bauer disk diffusion and MIC of vancomycin was determined by agar dilution method according to CLSI guidelines. STATISTICAL ANALYSIS: p value was calculated by Fisher's exact test. RESULTS: Amongst the total 90 Enterococcal isolates, 76 isolates (84.5 %) were Enterococcus faecalis, 13 isolates (14.4 %) were Enterococcus faecium and 1 isolate (1.1 %) was Enterococcus raffinosus. Out of the 90 isolates, 65 (72.2 %) were from urine, 15 (16.6 %) from pus, 5 (5.6 %) from blood and 5 (5.6 %) from endotracheal aspirates. High level gentamicin resistance was 22.4 % for E. faecalis and 30.8 % for E. faecium by Kirby Bauer disc diffusion method. Occurrence of vancomycin resistant enterococci (VRE) in our setting is 2.2 %. Isolates had 100 % sensitivity to linezolid. CONCLUSION: Enterococcus faecalis was the most common species. Maximum isolates were from urine samples. Higher level of gentamicin resistance could lead to failure of synergistic therapy. Speciation and regular monitoring for antibiotic resistance with special attention to vancomycin and high level aminoglycosides is warranted. KEYWORDS: Enterococci, speciation, antimicrobial resistance, high level gentamicin, vancomycin, MIC. INTRODUCTION:Enterococci have evolved over the past century, from being intestinal commensals to becoming pathogens associated with significant morbidity and mortality. 1 Enterococcus species cause urinary tract infections, bacteremia, endocarditis, intraabdominal and pelvic infections, wound and soft tissue infections, neonatal sepsis and rarely meningitis. 2 Enterococcus faecalis and Enterococcus faecium are the most common species accounting for 90-95 % of the clinical isolates and the other species E. gallinarum.

show abstract

Characterization and antimicrobial susceptibility pattern of clinical isolates of Enterococci at a tertiary care hospital in Mumbai, India

Cited by 14 publications

References 1 publication

Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Recent pattern of drug sensitivity of most commonly isolated uropathogens from Central India

Solation, Identification and Speciation of Enterococci and Their Antimicrobial Susceptibility in a Tertiary Care Hospital

Contact Info

Product

Resources

About