Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Sahu, Llalli S; Dash, Muktikesh; Paty, Bimoch Projna; Purohit, Gopal Krishna; Chayan, Nirupama

doi:10.21608/aeji.2015.17846

Cited by 1 publication

(1 citation statement)

References 27 publications

(21 reference statements)

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“…Multidrug-resistant enterococci have emerged as serious infections worldwide, especially vancomycin-resistant Enterococci (VRE), which have rapidly emerged in healthcare settings in recent decades. VRE were identified as hospital-associated pathogens in Europe in the mid-1980s and have since spread worldwide [ 4 ]. The drug resistance mechanism of VRE is mainly encoded by vanA and vanB gene clusters, which are most commonly found in Enterococcus faecium and are becoming widespread worldwide [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Vancomycin Resistant Enterococci and Drug Ligand Interaction between vanA of E. faecalis with the Bio-Compounds from Aegles marmelos

A.S,

Gunasekaran

et al. 2023

J Pharmacopuncture

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Objectives Enterococcus faecalis is a gram positive diplococci, highly versatile and a normal commensal of the gut microbiome. Resistance to vancomycin is a serious issue in various health-care setting exhibited by vancomycin resistant Enterococci (VRE) due to the alteration in the peptidoglycan synthesis pathway. This study is thus aimed to detect the VRE from the patients with root caries from the clinical isolates of E. faecalis and to evaluate the in-silico interactions between vanA and the Aegles marmelos bio-compounds. Methods E. faecalis was phenotypically characterized from 20 root caries samples and the frequency of vanA and vanB genes was detected by polymerase chain reaction (PCR). Further crude methanolic extracts from the dried leaves of A. marmelos was assessed for its antimicrobial activity. This is followed by the selection of five A. marmelos bio-compounds for the computational approach towards the drug ligand interactions. Results 12 strains (60%) of E. faecalis was identified from the root caries samples and vanA was detected from two strains (16%). Both the stains showed the presence of vanA and none of the strains possessed vanB . Crude extract of A. marmelos showed promising antibacterial activity against the VRE strains. In-silico analysis of the A. marmelos bio-compounds revealed Imperatonin as the best compound with high docking energy (–8.11) and hydrogen bonds with < 140 TPSA (Topological polar surface area) and zero violations. Conclusion The present study records the VRE strains among the root caries with imperatorin from A. marmelos as a promising drug candidate. However the study requires further experimentation and validation.

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Section: Introductionmentioning

confidence: 99%

Characterization of Vancomycin Resistant Enterococci and Drug Ligand Interaction between vanA of E. faecalis with the Bio-Compounds from Aegles marmelos

A.S,

Gunasekaran

et al. 2023

J Pharmacopuncture

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Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India

Abstract: Conclusion: More accurate and reliable MIC determination tests should be performed in all suspected VRE isolates. Confirmatory PCR is required for identifying resistant gene cluster.

Cited by 1 publication

References 27 publications

Characterization of Vancomycin Resistant Enterococci and Drug Ligand Interaction between vanA of E. faecalis with the Bio-Compounds from Aegles marmelos

Characterization of Vancomycin Resistant Enterococci and Drug Ligand Interaction between vanA of E. faecalis with the Bio-Compounds from Aegles marmelos

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