Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Jagoda, S. S. S. De S.; Wijewardana, TG; Arulkanthan, A.; Igarashi, Yuriko; Tan, Engkong; Kinoshita, Shigeharu; Watabe, Shugo; Asakawa, Shuichi

doi:10.3354/dao02733

Cited by 64 publications

(55 citation statements)

References 43 publications

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“…This find may be attributed to the intrinsic resistance of Aeromonas bacteria to penicillins, along with data from several studies (Dias et al 2012;Sreedharan et al 2012;Igbinosa 2014;Jagoda et al 2014). The susceptibility profile of the bacteria tested in the present study against penicillin G (10 IU), ampicillin (10 lg), amoxycillin (10 lg) and oxacillin (1 lg), all antibiotics from the penicillin class, demonstrated a massive resistance in all strains (data not shown).…”

Section: Discussionsupporting

confidence: 79%

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria

et al. 2018

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Section: Discussionsupporting

confidence: 79%

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria

et al. 2018

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“…Jagoda et al. () for example reported 18 different genotypes and emphasized the high heterogeneity in the distribution of virulence genes in motile aeromonads while Alves da Silva et al. () detected 29 profiles based on the presence of seven virulence genes in Aeromonas spp.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic, molecular and pathological characterization of motile aeromonads isolated from diseased fishes cultured in Uruguay

Perretta

Antúnez

Zunino

2018

Journal of Fish Diseases

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Information about motile aeromonads from aquaculture systems of the Neotropical region is scarce. The aim of this study was to characterize motile Aeromonas isolated from ornamental and consumable fishes cultured in Uruguay. Biochemical and molecular methods were used for species identification. Antimicrobial susceptibility and the presence of virulence genes were evaluated. Genetic diversity was analysed by rep-PCR, and virulence of the most representative isolates was determined by calculating the fifty lethal dose in experimentally challenged fish (Australoheros facetus). Aeromonas hydrophila and A. veronii were the most prevalent identified species (38.2% and 32.4%, respectively), whereas A. allosacharophila, A. bestiarium, A. caviae and A. punctata were less prevalent. This study constitutes the first report of these last four species in Uruguay. All isolates were resistant to at least three antimicrobials, and 82.3% of them showed multidrug resistance. Virulence genotypes were correlated with the Aeromonas species and haemolytic activity. The genotype act+/alt+/ast+/ela+/lip+ was the most prevalent (26.5%). A correlation between virulence genotypes and Aeromonas species was found. A. punctata showed a clonal structure according to rep-PCR analysis, whereas other species showed high genetic diversity. The number of virulence genes of the isolates was related with virulence according to the experimental challenge assays.

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“…veronii has emerged as an important opportunistic pathogen in humans and has been implicated in a number of intestinal and extra-intestinal infections in both immunocompromised and immunocompetent individuals [5], [6]. It has an established role as a fish pathogen and has been associated with motile Aeromonas septicaemia and ulcer syndrome in cultured fresh water food fish [7], [8] and tropical ornamental fish [9], [10], leading to severe economic losses. In recent years, A .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…veronii Ae52 on Tryptic Soy Agr (Difco) using the DNeasy Blood and Tissue kit (Qiagen). Sequence analysis of 16S rDNA, gyrB and rpoD genes was performed for confirmatory identification of the isolate prior to genome sequencing [10]. A genomic DNA library was constructed and sequenced on the Ion Torrent PGM (Life Technologies) platform using the ion 318 chip and 400-bp chemistry as per the manufacturer's instructions.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Genome sequencing and annotation of Aeromonas veronii strain Ae52, a multidrug-resistant isolate from septicaemic gold fish ( Carassius auratus ) in Sri Lanka

et al. 2017

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Here we report the draft genome sequence and annotation of A. veronii strain Ae52 isolated from the kidney of a morbund, septicaemic gold fish (Carassius auratus) in Sri Lanka. This clinical isolate showed resistance to multiple antimicrobials; amoxicillin, neomycin, trimethoprim-sulphonamide, chloramphenicol, tetracycline, enrofloxacin, erythromycin and nitrofurantoin. The size of the draft genome is 4.56 Mbp with 58.66% of G + C content consisting 4328 coding sequences. It harbors a repertoire of putative antibiotic resistant determinants that explains the genetic basis of its resistance to various classes of antibiotics. The genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession numbers BDGY01000001-BDGY01000080.

show abstract

Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Cited by 64 publications

References 43 publications

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria

Phenotypic, molecular and pathological characterization of motile aeromonads isolated from diseased fishes cultured in Uruguay

Genome sequencing and annotation of Aeromonas veronii strain Ae52, a multidrug-resistant isolate from septicaemic gold fish ( Carassius auratus ) in Sri Lanka

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