Characterization and Antimicrobial Activity of a Halophyte from the Asturian Coast (Spain): Limonium binervosum (G.E.Sm.) C.E.Salmon

Sánchez-Hernández, Eva; Buzón-Durán, Laura; Langa-Lomba, Natalia; Casanova-Gascón, José; Lorenzo-Vidal, Belén; Martín‐Gil, Jesús; Martín-Ramos, Pablo

doi:10.3390/plants10091852

Cited by 13 publications

(10 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…35 Phytol has antibacterial activity proved by Sánchez. 33 and n-Hexadecanoic acid as major components which is in the same line with the previous study carried out by Sánchez. 33 n-Hexadecanoic acid was proven to have bioactivities including antimicrobial and antioxidant activities.…”

Section: Resultssupporting

confidence: 92%

See 1 more Smart Citation

Antibacterial, Antioxidant Activities, GC-MS Analysis and Docking Studies of Guiera senigalensis (L.) Ethanol Leaves Extract

2023

TJNPR

View full text Add to dashboard Cite

The increment of Antimicrobial irrational use leads to the exacerbation of infections. The present study is designed to evaluate the antibacterial, and antioxidant activities of Guiera senigalensis ethanolic leaves extract and to determine the components of the extract for docking studies. G. senigalensis extract was used against Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, and Pseudomonas aeruginosa ATCC27853. The antimicrobial and antioxidant activities and active ingredients were determined using standard methods, computational studies were conducted via Maestro v12.8 of Schrodinger2020. Antibacterial activity of the crude extract showed that S. aureus was the most sensitive strain with MIC and MBC (12.5 and 25) mg/ml respectively followed by P. aeruginosa MIC and MBC (25 and 50) mg/ml. While E. coli was found to be resistant. In the DPPH radical scavenging assay of the extract and quercetin at concentrations (250, 125, 50, 10, and 5) µg/ml. The radical scavenging activity was 72.5%, 63.5%, 50.5%, 45.5%, and 31.3% for the extract, compared to quercetin which gave 89.7%, 85.8%, 62.1%, 55.5%, and 45% respectively. The GC-MS analysis of the total constituents revealed that 9H-Xanthene-9-one, 1,3,5,6-tetramethoxy-(28.81%), followed by phytol (22.74%), n-Hexadecanoic acid (11.3%) and 1,2,3-Benzenetriol (9.13) as major components. The docking studies of these major constituents support the antibacterial activity as they exhibited high binding affinity to UPPS targeted enzyme. Finally, this research provided useful information on the therapeutic potential of G. senegalensis. The major constituents could be considered potential antibacterial agents against S. aureus and recommended to be evaluated against MDR Bacterial strains.

show abstract

Section: Resultssupporting

confidence: 92%

“…These identified components in G. senegalensis leaves extract are known to possess high antioxidant activity, 19,32 as well as antibacterial effects. 17,18,33,34 The presence of the Xanthene derivative which known as bioactive with antibacterial activity according to Souza. 35 Phytol has antibacterial activity proved by Sánchez.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial, Antioxidant Activities, GC-MS Analysis and Docking Studies of Guiera senigalensis (L.) Ethanol Leaves Extract

2023

TJNPR

View full text Add to dashboard Cite

show abstract

“…In relation to the activity of COS, it is well established and may be ascribed to several mechanisms of action [37], including increased permeability of plasma membrane and the leakage of cellular contents, deprivation of trace elements essential for fungal normal growth due to its chelating action, and inhibition of mRNA synthesis and affection of protein and enzyme production due to binding to fungal DNA. As for the mechanism behind the high SFs observed for the conjugate complexes, synergism between COS and plant extracts may be explained by the fact that fungal pathogens are not resistant to multiple fungitoxicants [38], although solubility and bioavailability enhancements associated with conjugation have also been advocated in other works [39,40].…”

Section: Comparison Of In Vitro Activitymentioning

confidence: 99%

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

et al. 2022

Self Cite

View full text Add to dashboard Cite

Gray mold (Botrytis cinerea Pers.), crown and fruit rot (Phytophthora cactorum (Lebert and Cohn) J.Schröt), and verticillium wilt (Verticillium dahliae Kleb.) are among the main diseases that affect the strawberry crop. In the study presented herein, the bark extract of Uncaria tomentosa (Willd. ex Schult.) DC, popularly known as “cat’s claw”, has been evaluated for its capability to act as a sustainable control method. The bioactive compounds present in the aqueous ammonia extract were characterized by gas chromatography–mass spectroscopy, and the antimicrobial activity of the extract—alone and in combination with chitosan oligomers (COS)—was assessed in vitro and as a coating for postharvest treatment during storage. Octyl isobutyrate (30.7%), 19α methyl-2-oxoformosanan-16-carboxylate (9.3%), tetrahydro-2-methyl-thiophene (4.7%), and α-methyl manofuranoside (4.4%) were identified as the main phytoconstituents. The results of in vitro growth inhibition tests showed that, upon conjugation of the bark extract with COS, complete inhibition was reached at concentrations in the 39–93.75 μg∙mL−1 range, depending on the pathogen. Concerning the effect of the treatment as a coating to prolong the storage life and control decay during post-harvest storage, high protection was observed at a concentration of 1000 μg∙mL−1. Because of this effectiveness, higher than that attained with conventional synthetic fungicides, the bark extracts of cat’s claw may hold promise for strawberry crop protection.

show abstract

“…C.E. Salmon [74], which were previously tested in our research group against the same isolate, with inhibition values in the 750-1500 µg•mL −1 range. Finally, the lowest inhibitory levels for P. syringae pv.…”

Section: Comparison Of Efficacy With Other Natural Compoundsmentioning

confidence: 99%

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Sánchez-Hernández

García

Casanova-Gascón

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Cork, an anatomic adaptation of the bark of Quercus suber L. through its suberization process, finds its main application in the production of bottle stoppers. Its processing results in a large waste stream of cork fragments, granulates, and dust, which may be susceptible to valorization. The work presented here explored the use of its extracts to inhibit the growth of phytopathogenic microorganisms associated with apple tree diseases. The in vitro antimicrobial activity of cork aqueous ammonia extract was assayed against four fungi, viz. Monilinia fructigena and M. laxa (brown rot), Neofussicoccum parvum (dieback), and Phytophthora cactorum (collar and root rot), and two bacteria, viz. Erwinia amylovora and Pseudomonas syringae pv. syringae, either alone or in combination with chitosan oligomers (COS). Effective concentration values of EC90 in the 675–3450 μg·mL−1 range, depending on the fungal pathogen, were obtained in growth inhibition tests, which were substantially improved for the conjugate complexes (340–801 μg·mL−1) as a result of strong synergism with COS. Similar enhanced behavior was also observed in antibacterial activity assays, with MIC values of 375 and 750 μg·mL−1 for the conjugate complexes against P. syringae pv. syringae and E. amylovora, respectively. This in vitro inhibitory activity was substantially higher than those exhibited by azoxystrobin and fosetyl-Al, which were tested for comparison purposes, and stood out among those reported for other natural compounds in the literature. The observed antimicrobial activity may be mainly attributed to the presence of glycerin and vanillic acid, identified by gas chromatography–mass spectroscopy. In the first step towards in-field application, the COS–Q. suber bark extract conjugate complex was further tested ex situ against P. cactorum on artificially inoculated excised stems of the ‘Garnem’ almond rootstock, achieving high protection at a dose of 3750 μg·mL−1. These results suggest that cork industrial leftovers may, thus, be a promising source of bioactive compounds for integrated pest management.

show abstract

Characterization and Antimicrobial Activity of a Halophyte from the Asturian Coast (Spain): Limonium binervosum (G.E.Sm.) C.E.Salmon

Cited by 13 publications

References 73 publications

Antibacterial, Antioxidant Activities, GC-MS Analysis and Docking Studies of Guiera senigalensis (L.) Ethanol Leaves Extract

Antibacterial, Antioxidant Activities, GC-MS Analysis and Docking Studies of Guiera senigalensis (L.) Ethanol Leaves Extract

Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens

Valorization of Quercus suber L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases

Contact Info

Product

Resources

About