Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco

Ennaji, Hayat; Timinouni, Mohammed; Ennaji, My Mustapha; Hassar, Mohammed; Cohen, Nozha

doi:10.2147/idr.s3632

Cited by 17 publications

(6 citation statements)

References 33 publications

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“…This value is in agreement with those reported in Iran (2.99%) [18], Estonia (2.6%) [1] and Algeria (2.6%) [23]. However, it's higher than that found previously in Tetouan city of Morocco (1.5%) [24], in Japan (1.7%) [25] and India (1.5%) [26], and lower than that reported previously in Casablanca city of Morocco (23.3%) [27], Ireland (5.8 %) [28], Uruguay (11.2%) [29], China (21.7%) [30], Chile (25%) [31], Greece (14.3%) [32] and Spain (6.2%) [33]. Furthermore, traditional whey, minced mead, and raw sausage were the most contaminated foods (5.7%), followed by raw milk (3.8%), smen, bovine meat, poultry meat and raw fish (1.9%).…”

Section: Discussionsupporting

confidence: 91%

“…Furthermore, traditional whey, minced mead, and raw sausage were the most contaminated foods (5.7%), followed by raw milk (3.8%), smen, bovine meat, poultry meat and raw fish (1.9%). These results were higher than those reported in other regions of Morocco for poultry meat (6.6%), red meat products (6.5%), salads (6%) and seafoods (0%) [34], ground meat and sausage (3.3%) and raw poultry (1.3%) [27], raw milk (0.83%) [35], dairy products (0.74%) and poultry meat (0%) [24], and lower than those reported in poultry and bovine meat products (0% and 2.7%, respectively) [24], chiken meat (3.66%) [36], dairy products (4.1%) [34] and raw milk (8.33%) [37]. However, our findings are comparable to those reported in salads (0%) [24] and traditional whey (5.20%) [37].…”

Section: Discussioncontrasting

confidence: 64%

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Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods

Bouymajane

Filali

Oulghazi

et al. 2021

Heliyon

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Listeria monocytogenes is a pathogen contaminated food, it is the cause of listeriosis worldwide. The aims of this study were to investigate the occurrence, antimicrobial resistance, serotyping and virulence genes of L. monocytogenes isolated from foods in Meknes city of Morocco. From June 2017 to May 2018, 520 food samples were randomly collected from a traditional market and two overcrowded popular neighborhoods (Lahdim and Hamria) and subjected to the detection of L. monocytogenes. Then, the antimicrobial susceptibility of the isolated strains were evaluated using the standard disk diffusion method and the determination of serotypes and virulence genes was performed by PCR. The results showed the detection of L. monocytogenes in fifteen (2.9%) of 520 samples, including three (5.7%) isolates in traditional whey, raw minced meat and raw sausage, two (3.8%) in raw milk and one (1.9%) in smen (traditional butter), raw bovine meat, raw poultry meat and raw fish, while salads and rayeb (traditional coagulated milk) were not contaminated. Among the fifteen isolated L. monocytogenes, nine (60%) belonged to the serogroup (1/2a, 1/2c, 3a and 3c), two (13.3%) belonged to the serogroup (1/2b, 3b, 4b and 4d) and four (26.6%) do not belong to any studied serogroup. Furthermore, fifteen (100%) isolates showed the presence of actA gene, fourteen (93.3%) harbored hlyA, prfA and plcB genes, thirteen (86.7%) carried inlA and inlC genes and twelve (80%) showed inlJ gene. The antimicrobial susceptibility analysis showed that the isolated strains were more resistant to amoxicillin/clavulanic acid (67.0%), erythromycin (60.0%), sulphamethoxazole (40.0%), ampicillin and sulphamethoxazole/trimethoprim (33.0%) and tetracycline (20.0%). Furthermore, 66.7% (10/15) were multidrug-resistant. From this study, we can conclude that foods marketed in Meknes city were contaminated by multidrug-resistant strains of L. monocytogenes harboring virulence genes, which may cause a serious risk to public health.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 64%

Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods

Bouymajane

Filali

Oulghazi

et al. 2021

Heliyon

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“…All the tested L. monocytogenes showed 100% resistance to nalidixic acid as reported in the previous studies [45,46]. In contrast, Girma and Abebe [16] and Garedew et al [12] from Ethiopia, have reported 30.5% and 50% resistance, respectively, to nalidixic acid.…”

Section: Discussionsupporting

confidence: 61%

Characterization and Antimicrobial Susceptibility Patterns of Listeria monocytogenes from Raw Cow Milk in the Southern Part of Ethiopia

Hawaz,

Taye,

Muleta

2023

Journal of Food Quality

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Food safety remains the main health concern in the developing countries. Thus, the major purpose of the present study was to characterize and determine antibiotic susceptibility patterns of Listeria monocytogenes from raw milk samples collected from southern Ethiopia. Two hundred and forty raw cow milk samples were collected from dairy farms and smallholder dairy producers using a simple random sampling technique and analyzed by cultural and multiplex PCR methods. The antimicrobial susceptibility profile of L. monocytogenes was evaluated using the standard disk diffusion method. Over 28% of the samples were found positive for Listeria spp., of which 17 (7.08%) isolates were identified as L. monocytogenes after morphological and biochemical confirmation. The prevalence of L. monocytogenes was 6.02% in Hawassa city, 5.56% in Dale district, and 9.41% in Arsi Negele district. L. monocytogenes was higher in the wet season (9.32%) than in the dry season (4.92%). The gene for Listeria specific 16S rRNA was detected in all the 17 examined isolates, while hlyA and iapA were only found in 11 of them. Furthermore, no isolate was identified to have the prfA, actA, or plcA genes. Antimicrobial resistance profiling revealed that all the L. monocytogenes isolates were resistant to nalidixic acid (100%), followed by erythromycin (88.24%). However, all the L. monocytogenes isolates were sensitive to vancomycin, gentamicin, and sulfamethoxazole. Raw cow milk is a potential source of L. monocytogenes and it poses a threat to human and animal health. Therefore, it is crucial that dairy producers and vendors of raw milk in the study areas should take considerable precautions to prevent Listeria species from contaminating raw fresh milk.

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“…monocytogenes strains have emerged, and because most antibiotics cannot be given to pregnant patients with L . monocytogenes infections, we need to exploit natural therapeutic alternative to antibiotics [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Action of Oleanolic Acid on Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis

Kim

Lee

et al. 2015

PLoS ONE

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This study investigated the antimicrobial action of oleanolic acid against Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis. To determine the cytotoxicity of oleanolic acid, HEp-2 cells were incubated with oleanolic acid at 37oC. MICs (minimal inhibition concentrations) for L. monocytogenes, E. faecium, and E. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid at 2 × MIC. To investigate the mode of antimicrobial action of oleanolic acid, we measured leakage of compounds absorbing at 280 nm, along with propidium iodide uptake. Scanning electron microscope (SEM) images were also analysed. The viability of HEp-2 cells decreased (P < 0.05) at oleanolic acid concentrations greater than 128 μg mL-1. The MICs were 16-32 μg mL-1 for L. monocytogenes and 32-64 μg mL-1 for E. faecium and E. faecalis, and bacterial cell viability decreased (P < 0.05) about 3-4 log CFU mL-1 after exposure to 2 × MIC of oleanolic acid. Leakage of 280 nm absorbing materials and propidium iodide uptake was higher in oleanolic acid –treated cells than in the control. The cell membrane was damaged in oleanolic acid-treated cells, but the control group had intact cell membrane in SEM images. The results indicate that oleanolic acid can kill L. monocytogenes, E. faecium, and E. faecalis by destroying the bacterial cell membrane.

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Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco

Cited by 17 publications

References 33 publications

Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods

Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods

Characterization and Antimicrobial Susceptibility Patterns of Listeria monocytogenes from Raw Cow Milk in the Southern Part of Ethiopia

Antimicrobial Action of Oleanolic Acid on Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis

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