Characterization and Antibacterial Activity of 7S and 11S Globulins Isolated from Cowpea Seed Protein

Abstract

The present work was carried out to determine the characteristics and antibacterial activity of 7S and 11S globulins isolated from cowpea seed (Vigna unguiculata (L.) Walp.). The molecular mass of 7S globulin was demonstrated by SDS-PAGE bands to be of about 132, 129 and 95 kDa corresponding the α/, α and β subunits. The molecular mass of 11S globulin was demonstrated by SDS-PAGE bands to be existed between 28 and 52 kDa corresponding the basic and acidic subunits. The minimum inhibitory concentrations MICs of 7S and 11S globulins isolated from cowpea seed were determined against Gram positive bacteria viz: Listeria monocytogenes LMG 10470, Listeria ivanovii FLB 12, Staphylococcus aureus ATCC 25923 and Streptococcus pyogenes ATCC 19615, and Gram negative bacteria such as Klebsiella pneumonia ATCC 43816, Pseudomonas aeruginosa ATCC 26853, Escherichia coli ATCC 25922 and Salmonella ATCC 14028 using disc diffusion assay; they were showed to be in the range 10 to 200 µg/mL. Transmission electron microscope (TEM) examination of the protein-treated bacteria showed the antibacterial action of 11S globulin against S. typhimurium and P. aeruginosa was manifested by signs of cellular deformation, partial and complete lysis of cell components. Adding 11S globulin at both concentrations 50 and 100 µg/g to minced meat showed considerable decreases in bacterial counts of viable bacteria, psychrotrophs and coliforms compared to controls during 15 days storage at 4 °C, reflecting a promising perspective to use such globulin as a meat bio-preservative.

Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

“…Therefore, the identification of these bacteria was carried out by API kits (Biomerieux, Montalieu-Vercieu, France), which approved that the 18 presumptive S. aureus bacteria belong to S. aureus . These API-identification kits were used successfully for bacterial identification [ 8 , 10 , 11 ]. Identification of bacteria using API-kits is a well-established method for characterization and classification of bacteria to the species level as API-strips give accurate identification based on standardized extensive database (APIWEB TM serve) in safe and quick procedures as provided by Biomereux Company (Montaliea, France) [ 1 , 8 , 10 ].…”

“…This also might be due to osmotic pressure of the solutes which existed in hypertonic medium in relation to the outer aquatic medium; this facilitates the diffusion of the bioactive materials from cell membranes across the selective permeability. The lipophilic nature of some solutes facilitates their attachment to bacterial cell membranes which in turn causes cell death [ 8 , 9 , 35 ].…”

“…Mixtures of MSE with the antibiotic tetracycline were made as follows: (20 µg/mL MSE + 10 µg tetracycline) and (40 µg/mL MSE + 10 µg tetracycline). Filter paper discs of 6 mm diameter were soaked in each combination and the experiment was carried out as described above [ 8 ].…”

“…The severity of S. aureus infection is currently being increased due to emergence of multidrug resistant strains of S. aureus which are becoming endemic worldwide and are spreading into the community at large [ 8 ]. Vancomycin intermediate S. aureus (VISA) and vancomycin resistant S. aureus (VRSA) were isolated from different medicinal samples [ 8 ]. Therefore, inhibition of S. aureus by other alternatives is mandatory.…”

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

“…Rats weighting 125 ± 10 g were housed in wire cages at room temperature (25 ± 5 • C) (five animals/cage). Animals were given food and water regularly and experienced 12 h light/dark cycles throughout the experimental period by oral route according to previous study in this respect [30,[71][72][73]. Afteracclimatization, ratsweredividedintofourgroups (five rats for each).…”