Characterization and anti-tumor activity of exopolysaccharide produced by Lactobacillus kefiri isolated from Chinese kefir grains

Rajoka, Muhammad Shahid Riaz; Mehwish, Hafiza Mahreen; Fang, Huiyan; Padhiar, Arshad Ahmed; Zeng, Xierong; Khurshid, Mohsin; He, Zhendan; Zhao, Lingyun

doi:10.1016/j.jff.2019.103588

Cited by 77 publications

(40 citation statements)

References 66 publications

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“…Silver nanoparticles synthesized using EPS from Lactobacillus brevis demonstrates various applications in the biomedical field especially as a powerful anti-proliferative agents against various human cancer cell lines [110]. In-vitro anti-cancer activity exhibited by newly extracted MSR101 EPS from Lactobacillus kefiri on HT-29 cancerous cells showed satisfactory apoptotic mechanism through upregulation of the expression of cytochrome-c, BAX, BAD, caspase-3, caspase-8 and caspase-9 [111]. Acidic EPS produced by Lactobacillus strain SB27 showed maximal increase of capsase-3 activation inducing apoptosis, G0/G1 cell cycle arrest and anti-proliferative activity on HT-29 cells [112].…”

Section: Anticancer Activitymentioning

confidence: 99%

Exopolysaccharides from probiotic bacteria and their health potential

Angelin

Kavitha

2020

International Journal of Biological Macromolecules

315

225

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Exopolysaccharides (EPS) are extracellular macromolecules excreted as tightly bound capsule or loosely attached slime layer in microorganisms. They play most prominent role against desiccation, phagocytosis, cell recognition, phage attack, antibiotics or toxic compounds and osmotic stress. In the last few decades, natural polymers have gained much attention among scientific communities owing to their therapeutic potential. In particular the EPS retrieved from probiotic bacteria with varied carbohydrate compositions possess a plenty of beneficial properties. Different probiotic microbes have unique behavior in expressing their capability to display significant health promoting characteristics in the form of polysaccharides. In this new era of alternative medicines, these polysaccharides are considered as substitutes for synthetic drugs. The EPS finds applications in various fields like textiles, cosmetics, bioremediation, food and therapeutics. The present review is focused on sources, chemical composition, biosynthetic pathways of EPS and their biological potential. More attention has been given to the scientific investigations on antimicrobial, antitumor, anti-biofilm, antiviral, anti-inflammatory and immunomodulatory activities.

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Section: Anticancer Activitymentioning

confidence: 99%

Exopolysaccharides from probiotic bacteria and their health potential

Angelin

Kavitha

2020

International Journal of Biological Macromolecules

315

225

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“…Another study on the effect of Lentilactobacillus kefiri on the apoptosis induction on gastric cancer cells (AGS) showed its association with the decreased polarization of mitochondrial membrane potential (MMP) and decreased Bcl2 expression, which suggested its therapeutic potential for the treatment of gastric cancers [63]. In a recent study, Riaz Rajoka et al [10] characterized the isolated EPS produced by Lentilactobacillus kefiri MSR101 (MSR101 EPS) and studied their potential to block colon cancer (HT-29) cells growth. They found that MSR101 EPS illustrated anticancer activity against the HT-29 cancerous cell line and up-regulated the expression of Cyto-c, BAX, BAD, caspase3, caspase 8, and caspase 9.…”

Section: Anti-cancermentioning

confidence: 99%

“…According to the Food and Agriculture Organization of the United Nations (FAO) and World Health Organization (WHO), probiotics refer to live microorganisms which, when applied in sufficient amounts, bestow a health benefit to the host. Additionally, evidence has shown that kefir's exopolysaccharide, kefiran, has very significant physicochemical attributes and biological activities that certainly add value to the products [3,[8][9][10]. Existing reports have suggested important health benefits from kefir beverage consumption, such as anti-microbial, anti-tumor, anti-carcinogenic, hypocholesterolemic effects, anti-hypertensive, anti-diabetic, immunomodulatory activity, and also improving lactose digestion [11].…”

Section: Introductionmentioning

confidence: 99%

Kefir and Its Biological Activities

Azizi

Kumar

Yeap

et al. 2021

Foods

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Kefir is a fermented beverage with renowned probiotics that coexist in symbiotic association with other microorganisms in kefir grains. This beverage consumption is associated with a wide array of nutraceutical benefits, including anti-inflammatory, anti-oxidative, anti-cancer, anti-microbial, anti-diabetic, anti-hypertensive, and anti-hypercholesterolemic effects. Moreover, kefir can be adapted into different substrates which allow the production of new functional beverages to provide product diversification. Being safe and inexpensive, there is an immense global interest in kefir’s nutritional potential. Due to their promising benefits, kefir and kefir-like products have a great prospect for commercialization. This manuscript reviews the therapeutic aspects of kefir to date, and potential applications of kefir products in the health and food industries, along with the limitations. The literature reviewed here demonstrates that there is a growing demand for kefir as a functional food owing to a number of health-promoting properties.

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“…Although the host responses to the infectious microbes are thoroughly studied, the major concern is to explore safe and novel therapies using the beneficial microorganisms [10][11][12][13]. e production of novel bioengineered probiotics can be accomplished through genetic modifications which will not only enhance the efficacy of conventional probiotics but also lessen the pathogenic potential of these strains.…”

Section: Introductionmentioning

confidence: 99%

The Prospects for the Therapeutic Implications of Genetically Engineered Probiotics

Mazhar

Afzal

Almatroudi

et al. 2020

Journal of Food Quality

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The interest in the therapeutic use of probiotic microorganisms has been increased during the last decade although the doubts have ascended about the probiotics mainly because their beneficial effects are not fully understood, and, in many cases, their usefulness has not been validated in clinical trials. Consequently, the notion got a considerable interest in those strains having proven probiotic potential to be engineered for improvement in their beneficial features. The process of genetic engineering can also be used for probiotic strains for the reversion of antimicrobial resistance and other modifications for their safer and effective human applications. The lactic acid bacilli are predominantly opposite as they already have gained attention owing to their health-promoting benefits and their safety for human consumption; therefore, their use, especially as a delivery agent of vaccines and drugs, is gaining attention. The tailoring of probiotic strains will not only improve the data regarding the probiotic potential of these strains but also clinch the doubts concerning these probiotics. This article focuses on the approaches of bioengineered probiotics and discusses the potential prospects for their therapeutic applications including immunomodulation, cognitive health, and anticancer therapeutics.

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Characterization and anti-tumor activity of exopolysaccharide produced by Lactobacillus kefiri isolated from Chinese kefir grains

Cited by 77 publications

References 66 publications

Exopolysaccharides from probiotic bacteria and their health potential

Exopolysaccharides from probiotic bacteria and their health potential

Kefir and Its Biological Activities

The Prospects for the Therapeutic Implications of Genetically Engineered Probiotics

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