Characterization and anti-tumor activity of saponin-rich fractions of South Korean sea cucumbers (Apostichopus japonicus)

Dai, Yulin; Kim, Eun-A; Luo, Haoming; Jiang, Yunfei; Oh, Jae-Young; Heo, Soo‐Jin; Jeon, You‐Jin

doi:10.1007/s13197-020-04266-z

Cited by 25 publications

(22 citation statements)

References 27 publications

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“…Saponins are important active components of sea cucumber, which have been used in the functional foods and nourishing industries (Dai et al., 2020). Figure 9d shows the change of saponins content of sea cucumber after the MVD treatment with different powers of 150, 200, and 250 W. The saponins contents of fresh sea cucumber and samples treated by 150, 200, and 250 W were 1.2901, 1.2901, 1.2902, and 1.2902 µg/g, respectively.…”

Section: Resultsmentioning

confidence: 99%

Effects of microwave vacuum drying on the moisture migration, microstructure, and rehydration of sea cucumber

Xiu

Cheng

et al. 2021

Journal of Food Science

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Effectsof microwave vacuum drying (MVD) on moisture migration, microstructure, and rehydration of sea cucumber were investigated in this paper. Vacuum condition avoided the exposure of sea cucumber to high temperature. Low‐field nuclear magnetic resonance relaxation results revealed that the peaks of three water components in sea cucumber shifted to short relaxation time during MVD process, and the peak area of major water component—immobilized water—decreased significantly due to water evaporation. Magnetic resonance imaging found that the water in the internal layer of sea cucumber body wall was first removed due to the internal heating of microwave, and then the water in the outer layer. Higher microwave power could promote the moisture transfer motion during drying process, and shorten the drying time. Porous microstructure was observed by Cryo scanning electronic microscope images in sea cucumber dried with microwave power of 200 and 250 W, which might be responsible for high values of rehydration ratio and water holding capacity. High microwave power caused the increase of amino acids content, but had no significant effect on the change of saponins content. In addition, excellent prediction models of moisture ratio have been developed by partial least squares regression analysis based on transverse relaxation data, which proved the feasibility of low‐field nuclear magnetic resonance to monitor moisture changes of sea cucumber during MVD process. Practical Application Effects of microwave vacuum drying (MVD) on moisture migration, microstructure, and rehydration of sea cucumber were investigated. Understanding the impacts of MVD drying on water status, texture, and nutritional characteristics of sea cucumber is important to improve the processing quality of dried sea cucumber.

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Section: Resultsmentioning

confidence: 99%

Effects of microwave vacuum drying on the moisture migration, microstructure, and rehydration of sea cucumber

Xiu

Cheng

et al. 2021

Journal of Food Science

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“…In particular, polyphenols and proteins from marine resources have increased free radical scavenging activity and have been shown to inhibit the effects of lipid peroxidation (Cian et al 2013). Compared to the synthetic drug for antioxidant, marine product becomes more flexible to obtain (Hamann 2007, Dai et al 2020b. Thus, the exploration and screening the marine antioxidants is necessary to investigate.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of extracts from six local strains of <italic>Pyropia yezoensis</italic> against oxidative damage <italic>in vitro</italic> and in zebrafish model

Dai¹,

Kim²,

Kang³

et al. 2020

ALGAE

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Pyropia yezoensis has been used as functional food in East Asia, especially in Korea and Japan, for more than five hundred years. This study aims to evaluate the antioxidant effect of polyphenols and proteins-rich extracts from P. yezoensis (PPPs) against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative cell damage. Among six Korean local strains obtained from Jinhae (JiH), Haenam (HN), Jangheung (JaH), Jindo (JD), Wando (WD), and Sinan (SA) areas, the extracts of P. yezoensis from SA and JD are relatively higher in polyphenols and proteins contents. SA showed the lowest IC 50 scavenging activities against 1,1-diphenyl-2-picryl-hydrazyl and alkyl radicals and displayed protective effects against reactive oxygen species (ROS) in AAPH-induced Vero cells. Especially, the PPPs extracts from SA and JD showed protective activities against AAPH-induced apoptosis, as observed by nuclear staining with Hoechst 33342. Furthermore, in vivo studies of the SA extract in zebrafish showed significantly reduced ROS generation, lipid peroxidation, and cell damage. This is the first study, to our knowledge, to evaluate the antioxidant bioactivity of PPP in the Korean Peninsula using a zebrafish model. Due to SA and JD both located in the west coast of Korea, we deduced that the chemical content of the different PPP extracts was mildly influenced by their geographic location, and this alga has potential of protective activity against AAPH-induced ROS both in vitro and in vivo.

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“…Next, a 25-μL volume of Hoechst 33342 (stock 1 mg/mL)/PI (5 μg/mL) nuclear staining solution and a 25-μL volume of DCFH-DA solution (25 μM) were added to cells for 24 h incubation. Cells were imaged using a fluorescence microscope coupled with a CoolSNAP-Pro color digital camera (Meyer Instruments, Inc., Houston, TX, USA) after 10 min of staining in the dark [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo

Dai

Jiang

et al. 2021

Toxicology Reports

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Particulate matter (PM) contributes to air pollution and primarily originates from unregulated industrial emissions and seasonal natural dust emissions. Fucoxanthin (Fx) is a marine natural pigment from brown macroalgae that has been shown to have various beneficial effects on health. However, the effects of Fx on PM-induced toxicities in cells and animals have not been assessed. In this study, we investigated the anti-inﬂammatory potential of the Fx-rich fraction (FxRF) of Sargassum fusiformis against PM-mediated inﬂammatory responses. The FxRF composition was analyzed by rapid-resolution liquid chromatography mass spectrometry. Fx and other main pigments were identified. FxRF attenuated the production of inﬂammatory components, including prostaglandin E2 (PGE 2 ), cyclooxygenase-2, interleukin (IL)-1β, and IL-6 from PM-exposed HaCaT keratinocytes. PM exposure also reduced the levels of nitric oxide (NO), tumor necrosis factor-α, inducible nitric oxide synthase (iNOS), and PGE 2 in PM-exposed RAW264.7 macrophages. Additionally, the culture medium from PM-exposed HaCaT cells induced upregulation of NO, iNOS, PGE 2 , and pro-inﬂammatory cytokines in RAW264.7 macrophages. FxRF also significantly decreased the expression levels of factors involved in inﬂammatory responses, such as NO, reactive oxygen species, and cell death, in PM-exposed zebrafish embryos. These results demonstrated the potential protective effects of FxRF against PM-induced inflammation both in vitro and in a zebrafish model.

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Characterization and anti-tumor activity of saponin-rich fractions of South Korean sea cucumbers (Apostichopus japonicus)

Cited by 25 publications

References 27 publications

Effects of microwave vacuum drying on the moisture migration, microstructure, and rehydration of sea cucumber

Effects of microwave vacuum drying on the moisture migration, microstructure, and rehydration of sea cucumber

Protective effects of extracts from six local strains of <italic>Pyropia yezoensis</italic> against oxidative damage <italic>in vitro</italic> and in zebrafish model

Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo

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