“…The cells were treated with 25 µg mL −1 sample and 1 µg LPS/mL at 37 °C for 24 h. Cells treated with 1 µg mL −1 LPS and 100 µM indomethacin were used as the control. Then, 80 µL of culture supernatant was mixed with the same volume of Griess reagent, held at room temperature for 20 min, then measured for absorbance at 540 nm ( Lee et al, 2020 , Xu et al, 2021 ).…”