Characteristics of the monocistronic genome of extra small virus, a virus-like particle associated with Macrobrachium rosenbergii nodavirus: possible candidate for a new species of satellite virus

Widada, Joannes Sri; Bonami, Jean-Robert

doi:10.1099/vir.0.79777-0

Cited by 71 publications

(57 citation statements)

References 12 publications

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“…Nucleotide sequencing of the XSV genome showed that the viral RNA is composed of 796 nucleotides and in sense [25]. XSV genome contained the coding sequence of the capsid protein CP-17 or CP-16 and no other coding sequences were found.…”

Section: Causative Organismsmentioning

confidence: 99%

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

Hameed¹,

Bonami

2012

Indian J. Virol.

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Macrobrachium rosenbergii is the most important cultured freshwater prawn in the world and it is now farmed on a large scale in many countries. Generally, freshwater prawn is considered to be tolerant to diseases but a disease of viral origin is responsible for severe mortalities in larval, post-larval and juvenile stages of prawn. This viral infection namely white tail disease (WTD) was reported in the island of Guadeloupe in 1995 and later in Martinique (FrenchWest Indies) in Taiwan, the People's Republic of China, India, Thailand, Australia and Malaysia. Two viruses, Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus-like particle (XSV) have been identified as causative agents of WTD. MrNV is a small icosahedral non-enveloped particle, 26-27 nm in diameter, identified in the cytoplasm of connective cells. XSV is also an icosahedral virus and 15 nm in diameter. Clinical signs observed in the infected animals include lethargy, opaqueness of the abdominal muscle, degeneration of the telson and uropods, and up to 100 % within 4 days. The available diagnostic methods to detect WTD include RT-PCR, dot-blot hybridization, in situ hybridization and ELISA. In experimental infection, these viruses caused 100 % mortality in post-larvae but failed to cause mortality in adult prawns. The reported hosts for these viruses include marine shrimp, Artemia and aquatic insects. Experiments were carried out to determine the possibility of vertical transmission of MrNV and XSV in M. rosenbergii. The results indicate that WTD may be transferred from infected brooders to their offspring during spawning. Replication of MrNV and XSV was investigated in apparently healthy C6/36 Aedes albopictus and SSN-1 cell lines. The results revealed that C6/36 and SSN-1cells were susceptible to these viruses. No work has been carried out on control and prevention of WTD and dsRNA against protein B2 produced RNAi that was able to functionally prevent and reduce mortality in WTD-infected redclaw crayfish.

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Section: Causative Organismsmentioning

confidence: 99%

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

Hameed¹,

Bonami

2012

Indian J. Virol.

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“…The culture of M. rosenbergii in India is declining due to disease outbreaks, especially white tail disease (WTD). WTD is caused by M. rosenbergii nodavirus (MrNV) and an associated satellite virus, extra small virus (XSV) [6,10]. The disease affects larval, post-larval and early juvenile stages causing up to 100 % mortality.…”

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confidence: 99%

“…Its genome is composed of two fragments of linear single-stranded RNA (ss-RNA), of 2.9 and 1.3 kb, respectively and its capsid is made up of a single polypeptide of 43 kDa [2]. MrNV is associated with a small virus, XSV, a non-enveloped icosahedral virus, 15 nm in diameter [10]. The exact role of these viruses in the disease pathogenesis or their interaction is not completely understood.…”

mentioning

confidence: 99%

“…The exact role of these viruses in the disease pathogenesis or their interaction is not completely understood. The XSV is classified as a satellite virus owing to its very small size, lack of genes encoding RNA polymerase for replication and therefore, is dependant on the helper virus (MrNV) and possesses a single gene encoding the structural protein [10]. Sequencing of the XSV genome showed that it consists of a linear single-stranded RNA of 796 nucleotides.…”

mentioning

confidence: 99%

“…The genome is in sense orientation, with a short poly (A) tail at the 3 0 -end. The genome has an ORF of 522 nucleotides coding for a 174 amino acid polypeptide of 17 kDa and a truncated protein of 16 kDa lacking the first 11 N-terminal amino acids [2,10].…”

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confidence: 99%

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Production and Application of Polyclonal Antibodies Against Recombinant Capsid Protein of Extra Small Virus of Macrobrachium rosenbergii

et al. 2012

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Macrobrachium rosenbergii nodavirus along with a satellite virus, extra small virus (XSV) causes white tail disease (WTD) in the giant freshwater prawn M. rosenbergii. Infected M. rosenbergii postlarvae were collected from a hatchery in Kakinada, Andhra Pradesh. The gene coding the capsid protein of XSV was cloned in a bacterial expression vector pRSET A and the recombinant protein was expressed in Escherichia coli BL21(DE3)pLysS cells. The recombinant protein was purified by Nickel affinity chromatography. Polyclonal antibodies were produced in mice against the recombinant protein and the antibodies reacted specifically with the recombinant protein and XSV in WTD-infected tissues. This is the first report of detection of XSV using antibodies against recombinant capsid protein.

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