Characteristics of Sertoli cells in the ectopic and scrotal testes of unilateral cryptorchid West African dwarf goats

Godwin, Ozoegwu Chigbogu; Daniel, E N

doi:10.5603/fm.a2015.0124

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…In the cryptorchid male gonad, the localization of OXB and OX2R is restricted to Leydig and Sertoli cells. That might be attributed to detrimental effects on spermatogenesis, due to the undescending testis which ultimately leads to infertility as already reported (21,(35)(36)(37).…”

Section: Discussionmentioning

confidence: 61%

Does Orexin B-Binding Receptor 2 for Orexins Regulate Testicular and Epididymal Functions in Normal and Cryptorchid Dogs?

et al. 2022

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Orexins A (OXA) and B (OXB) and the receptors 1 (OX1R) and 2 (OX2R) for orexins are hypothalamic peptides found in several mammalian organs and participated to the control of a wide assortment of physiological and pathological functions. The distribution of OXA and OX1R has been extensively studied in the male gonad of mammals. Here, we examined the expression and localization of OXB and OX2R as well as their possible involvement in the regulation of testicular and epididymal functions, in healthy and cryptorchid dogs, employing some techniques such as immunohistochemistry, Western blotting, and real-time RT-PCR. In vitro tests were also carried out for evaluating the steroidogenic effect of OXB. OXB and OX2R were expressed in spermatocytes, spermatids, and Leydig cells in normal testis. Their localization was restricted to Sertoli and Leydig cells in cryptorchid conditions. OXB was found to be localized in all tracts of both normal and cryptorchid epididymis, whereas OX2R was found only in the caput. Because the small molecular weight of the peptides OXA and OXB, the expression of their precursor prepro-orexin (PPO), OX1R, and OX2R proteins and mRNAs were investigated by means of Western blot and real-time RT-PCR analyses, respectively, in all tested groups of. In particular, the mRNA level expression of all three genes was higher in cryptorchid dogs than in normal ones. In vitro tests demonstrated that OXB—by binding OX2R—is not involved in testicular steroidogenic processes. Therefore, the findings of this study might be the basis for further functional and molecular studies addressing the possible biochemical effects of OXB and OX2R in normal and pathological conditions of the male reproductive system.

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Section: Discussionmentioning

confidence: 61%

Does Orexin B-Binding Receptor 2 for Orexins Regulate Testicular and Epididymal Functions in Normal and Cryptorchid Dogs?

et al. 2022

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“…The widespread expression of OXB and OX2R in numerous cytotypes in the testis of healthy dogs and their localization pattern closely resembled the previous results in rats and alpacas [ 13 , 14 ], allowing for the hypothesis of a potential role in the regulation of spermatogenesis and endocrine functions in the canine testicle through a mechanism involving autocrine and/or paracrine action. In the retained gonad from cryptorchid dogs, the distribution of OXB and OX2R was limited to Leydig and Sertoli cells, which may be ascribed to damaging consequences of the undescending testis on spermatogenesis, leading to infertility [ 99 , 100 , 101 , 102 ]. In addition, OXB/OX2R did not appear to have a direct steroidogenic effect on the testis, as reported in the normal rat male gonad too [ 13 ].…”

Section: The Orexinergic System In the Male Genital Tract Of Dogmentioning

confidence: 99%

A Comparative Analysis of Orexins in the Physio-Pathological Processes of the Male Genital Tract: New Challenges? A Review

Costagliola,

Montano,

Langella

et al. 2024

Veterinary Sciences

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Orexins A (OXA) and B (OXB) and their specific receptors, receptor 1 (OX1R) and 2 (OX2R) for orexins, are hypothalamic peptides involved in orchestrating several functions in the central nervous system and peripheral organs, including sleep, excitement, nutrition, reward, circadian rhythm, anxiety, cognition, and reproduction. The aim of this narrative review is, in particular, to speculate the role of orexins in the male genital tract of animal species and human beings. The experimental evidence collected in recent years assumed that in the testes of the animal species here described, orexins are directly involved in steroidogenesis and spermatogenesis regulation. In the epididymis, these peptides are locally synthesized, thus suggesting their role governing the fertilizing capability of the immature male gamete. In addition to playing a physiological role, orexins are involved in numerous inflammatory and/or neoplastic pathologies too. The expression of the orexinergic system in prostate cancer suggests that they might play a potential therapeutic function. Overall, the future directions of this literature review allow us to hypothesize a role of the orexinergic complex not only as a marker for the diagnosis of certain tumors affecting the male genital tract but also for the treatment of hypo/infertility condition.

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Characteristics of Sertoli cells in the ectopic and scrotal testes of unilateral cryptorchid West African dwarf goats

Abstract: (Folia Morphol 2016; 75, 3: 355-363)

Cited by 2 publications

References 21 publications

Does Orexin B-Binding Receptor 2 for Orexins Regulate Testicular and Epididymal Functions in Normal and Cryptorchid Dogs?

Does Orexin B-Binding Receptor 2 for Orexins Regulate Testicular and Epididymal Functions in Normal and Cryptorchid Dogs?

A Comparative Analysis of Orexins in the Physio-Pathological Processes of the Male Genital Tract: New Challenges? A Review

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