Characteristics of Prolactin Secretion in Normal and Estrogen-Treated Pituitaries of Rats at the Single Cell Level: Analysis by Reverse Hemolytic Plaque Assay.

Yoshikawa, Mineka; Uozumi, Tohru; Kawamoto, Keiichi; Arita, Kazunori; Ito, Akihiro; Takahashi, Sumio

doi:10.1507/endocrj.42.235

Cited by 3 publications

(2 citation statements)

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“…These results agree with a previous report showing no differences in basal PRL secretion between E 2 -induced adenoma and normal pituitaries, estimated by RIA (Yoshikawa et al 1995). In addition, these authors found a smaller mean plaque area for E 2 -treated cells from F344 rats using the reverse hemolytic plaque assay (RHPA), suggesting a lower PRL release rate in E 2 -induced tumor cells (Yoshikawa et al 1995). This observation could be in line with the low levels of exocytotic protein components detected in pituitary glands of E 2 -treated rats.…”

Section: Discussionsupporting

confidence: 93%

“…Our results show that neither the basal nor 8-Br-cAMP-induced PRL release in cultured cells from E 2 -treated animals differs from that in control donors. These results agree with a previous report showing no differences in basal PRL secretion between E 2 -induced adenoma and normal pituitaries, estimated by RIA (Yoshikawa et al 1995). In addition, these authors found a smaller mean plaque area for E 2 -treated cells from F344 rats using the reverse hemolytic plaque assay (RHPA), suggesting a lower PRL release rate in E 2 -induced tumor cells (Yoshikawa et al 1995).…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Exocytotic protein components in rat pituitary gland after long-term estrogen administration

Majó

Lorenzo²,

Blasi³

et al. 1999

Journal of Endocrinology

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Recently, a set of proteins involved in the docking and fusion machinery of secretory organelles has been identified in anterior pituitary cells. In this study we analyzed, by Western blotting and immunocytochemistry, the expression of several proteins involved in exocytosis after long-term administration of 17 -estradiol (E 2 ) in Fischer 344 rats. No differences were observed in the amount of synaptosomal-associated protein of 25 kDa, synaptobrevin 2, syntaxin 1, synaptotagmin I and Rab3a in total brain homogenates from treated rats after E 2 administration. In striking contrast, the levels of all of these exocytotic proteins, including cellubrevin, were notably decreased in pituitary glands of E 2 -treated rats. In addition, no differences were observed in the in vitro basal and 8-Br-cAMPinduced prolactin (PRL) release between pituitary cells from control and E 2 -treated rats, whereas TRH-induced PRL release in anterior pituitary cells from E 2 -treated animals was higher than in control donors. In conclusion, this study shows that protein components of the exocytotic machinery are specifically down-regulated in the pituitary gland of E 2 -treated Fischer 344 rats.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%