Characteristics of periodontal ligament subpopulations obtained by sequential enzymatic digestion of rat molar periodontal ligament

Kaneda, Takashi; Miyauchi, Mutsumi; Takekoshi, Toshitsugu; Kitagawa, Shoji; Kitagawa, Masae; Shiba, Hideki; Kurihara, Hidemi; Takata, Takashi

doi:10.1016/j.bone.2005.08.021

Cited by 52 publications

(44 citation statements)

References 16 publications

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“…PDL cells isolated by sequential enzymatic digestion have been reported to exhibit different proliferation, ALP, and mineralization activities (Kaneda et al, 2006). Moreover, ALP activity and collagen type I expression were shown to be strongest adjacent to the bone, while cell proliferation was not significant in the PDL adjacent to the bone in vivo (Rooker et al, 2010).…”

Section: Discussionmentioning

confidence: 98%

Osteogenic differentiation regulated by Rho-kinase in periodontal ligament cells

et al. 2014

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Section: Discussionmentioning

confidence: 98%

Osteogenic differentiation regulated by Rho-kinase in periodontal ligament cells

et al. 2014

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“…It's a kind of heterogeneous pluripotent cells with self-renewal ability, including mature cells that have different functions and undifferentiated mesenchymal cells that have differentiation potentials. [15–17] Hypoxia is a basic environment of life development, and it affects the survival of cells and the formation of blood vessels through a series of regulatory mechanism, which is critical to periodontal tissue reconstruction. [18] To better understand the mechanisms of hypoxia on hPDLCs and to provide novel therapies for periodontal diseases, there are increasingly researches to explore the effects of hypoxia on PDLCs.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway

et al. 2017

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Background:The aim of this study is to investigate the effects of hypoxia on the proliferation, morphology, migration ability, hypoxia inducible factor (HIF) 1 (HIF-1) expression, and the relationship with Wnt/β-catenin signaling of human periodontal ligament cells (hPDLCs) in vitro.Methods:hPDLCs (4th passage) cultured by the tissue culture method were randomly assigned to slight (5% O2), severe hypoxia (1% O2) groups, and the control (21% O2) group, respectively. From 1st to 7th day, the optical density values were detected, and the growth curve was described. Wound healing assay was done to observe the migration ability of hPDLCs under various O2 conditions. Then reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of cementum-related genes and Wnt signaling pathway-related genes. Further, RT-qPCR, Western blot, and immunofluorescence staining method were constructed to show HIF expressions under different O2 concentrations in hPDLCs.Results:The growth rate of hPDLCs decreased with the reduction of O2 content by degree, and the morphology of hPDLCs changed in different O2 contents. Besides, hPDLCs migrate faster in 21% and 5% O2 than in 1% O2, and both the expressions of cementum-related genes and Wnt signaling pathway-related genes were raised under hypoxic conditions. In addition, with the reduction of O2 concentration, the messenger RNA and protein level expression of HIF were all increased, and HIF was gradually transported from cytoplasm into the nucleus and in 1% O2 concentration, it was mainly expressed in the nucleus.Conclusion:This finding demonstrated that hypoxia was capable of suppressing the proliferation and migration ability, changing the morphology of hPDLCs, and stabilizing HIF-1α against degradation and promoting its translocation to the nucleus. Meanwhile, hypoxia may regulate hPDLCs proliferation and cementogenic differentiation via Wnt/β-catenin signaling pathway, which may potentially provide a novel insight into the etiology and treatment of periodontal diseases.

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“…Primers for ALP, type I collagen (Col I), osteocalcin (ON) (11), osteopontin (OPN) (12), bone sialoprotein (BSP), dentin matrix protein-1 (DMP-1), and the internal calibrator GAPDH (synthesized by Invitrogen, Shanghai, China) are listed in Table 1. A 20 mL PCR reaction was amplified in a Roche Lightcycler (Roche Diagnostics, Mannheim, Germany).…”

Section: Real Time Pcr Of Mineralization-related Gene Expressionmentioning

confidence: 99%

Effect of Wnt6 on Human Dental Papilla Cells In Vitro

Chen¹,

Ren²,

Peng³

et al. 2010

Journal of Endodontics

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Characteristics of periodontal ligament subpopulations obtained by sequential enzymatic digestion of rat molar periodontal ligament

Cited by 52 publications

References 16 publications

Osteogenic differentiation regulated by Rho-kinase in periodontal ligament cells

Osteogenic differentiation regulated by Rho-kinase in periodontal ligament cells

Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway

Effect of Wnt6 on Human Dental Papilla Cells In Vitro

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