Effect of Wnt6 on Human Dental Papilla Cells In Vitro

KLF4 Promotes the Odontoblastic Differentiation of Human Dental Pulp Cells

“…For example, the hepatocyte growth factor was detected to enhance the proliferation and differentiation of DPCs by up-regulating cAMP response elementbinding (CREB) and Ets-like protein 1 (Elk-1) (23). Wnt6 proved to promote odontoblastic differentiation without affecting cell proliferation (24). Transforming growth factor (TGF)-b2 was believed to inhibit the differentiation of hDPCs through activation of the ALK/Smad2/3-signal transduction pathways (25), but only a small number of transcription factors has been found to influence odontoblastic differentiation of hDPCs.…”

Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

“…The mRNA and protein levels of both factors were increased by Poly(P). Bone-associated cells also express other Wnt proteins such as Wnt3a, Wnt6, Wnt10a, and Wnt11 (41)(42)(43). To assess whether the induction of Wnt5a and Wnt5b by Poly(P) is a specific response in DPFCs, we evaluated the expression of these other Wnt proteins following treatment with the same concentrations of Poly(P).…”

JNK/P38 Mitogen-activated Protein Kinase Used for Hepatocyte Growth Factor–induced Proliferation, Differentiation, and Migration in Human Dental Papilla Cells

“…After 1, 2, and 3 days of culture, the cells were lysed by treating with 0.1 mol/L of Triton X-100 for 2 hours, and lysates were centrifuged at 5000 rpm for 10 minutes. The supernatants were used to determine the alkaline phosphatase (ALP) activity by using an ALP assay kit (Nanjing Jiancheng Technological Inc, Nanjing, Jiangsu, China) as described (16). This experiment was repeated 3 times.…”

“…HGF was added in the experimental groups with pretreatment of SB203580 or SP600125, whereas the controls were not treated with HGF. The degree of mineralization was determined by using alizarin red staining after 2 and 3 weeks of culture as described (16). The experiment was repeated 3 times.…”