Characteristics of melanomacrophage centers in the liver and spleen of the roach Rutilus rutilus (Cypriniformes: Cyprinidae) in Lake Kotokel during the Haff disease outbreak

Pronina, S. V.; Batueva, M. D.; Pronin, N. M.

doi:10.1134/s003294521401010x

Cited by 19 publications

(13 citation statements)

References 34 publications

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“…In the present study, three species had absence of division into hepatic lobules and the lack of portal triads as also recorded in many teleosts (Vicentini et al, 2005;Nejedli, Gajger, 2013;Pronina et al, 2014;Faccioli et al, 2014). The network of blood capillaries showed vessels that were anastomosed and drained by centre-lobular veins.…”

Section: Discussionmentioning

confidence: 77%

“…In teleosts, they are particularly concentrated in the head kidney, liver and spleen from some species (Wolke, 1992;Ribeiro et al, 2011;Pronina et al, 2014;Diaz-Satizabal, Magor, 2015;Gomes et al, 2015). The liver and spleen of G. brasiliensis and H. aff.…”

Section: Discussionmentioning

confidence: 99%

“…It is often found in fish liver (Wolke, 1992;Velkova-Jordanoska, Kostoski, 2005;Leknes, 2015). Increased hemosiderin in hepatocytes (hemosiderosis) was found in teleosts in a disturbed environment (Pronina et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, variations in the size, number and histology of the MMCs can occur due to age, nutritional status, disease and environmental conditions (Hartley et al, 1996, Ribeiro et al, 2011Pronina et al, 2014). In O. niloticus, number and area of splenic MMCs were directly related to type of inflammatory agent and that the increases in area and number of MMCs were greater when the stimulation was performed by inoculation of the bacillus Calmette-Guerin (BCG) when compared to subcutaneous implant of glass coverslip or with the implant and BCG in association (Manrique et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The analysis and quantification of granules can be a great indicator of the main metabolic activities by macrophages from MMCs (Ribeiro et al, 2011;Diaz-Satizabal, Magor, 2015;Gomes et al, 2015). Moreover, presence, number and area of MMCs have been used as biological parameters in toxicology and environmental impact studies (Rabitto et al, 2004;Ribeiro et al, 2011;Pronina et al, 2014;Gomes et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Comparative histology in the liver and spleen of three species of freshwater teleost

et al. 2017

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We describe and compare the histology of liver and spleen of Geophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) and Hoplias aff. malabaricus (Characiformes), tropical freshwater fishes. In G. brasiliensis and H. aff. malabaricus the hepatocytes were arranged in tubular form whereas in H. francisci they cord-like. In all species, hepatocytes presented glycogen, but in G. brasiliensis and H. aff. malabaricus they showed strong stained for hemossiderin in the cytoplasm. In G. brasiliensis and H. aff. malabaricus, melanomacrophage centres (MMCs) were associated to hepatic structures and only in G. brasiliensis was observed intrahepatic exocrine pancreas. The spleen, in all species, was characterized by red and white pulp without boundary between the two regions, but only in H. francisci was recorded nodular organization in splenic parenchyma. The G. brasiliensis and H. aff. malabaricus presented in the white pulp MMCs linked mainly to ellipsoids. Besides, we observed large MMCs in the spleen in relation to liver of G. brasiliensis and H. aff. malabaricus. In liver, highest values of reticular fibers and collagen were observed in G. brasiliensis. In spleen, highest values of reticular fibers and collagen were recorded in H. aff. malabaricus and H. francisci, respectively. Histological differences confirm the hypothesis that the phylogenetic distance is reflected in liver and spleen.Keywords: Extracellular matrix, Hepatocytes, Intrahepatic exocrine pancreas, Melanomacrophage centres, Red and White pulp.Nós descrevemos e comparamos a histologia do fígado e do baço de Geophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) e Hoplias aff. malabaricus (Characiformes), peixes neotropicais de água doce. Em G. brasiliensis e H. aff. malabaricus os hepatócitos organizaram-se na forma tubular enquanto que em H. francisci eles apresentaram-se como cordões celulares. Em todas as espécies, os hepatócitos apresentaram glicogênio, mas em G. brasiliensis e H. aff. malabaricus, eles mostraram forte marcação para hemossiderina no citoplasma. Em G. brasiliensis e H. aff. malabaricus, centros melanomacrofágicos (CMMs) foram associados a estruturas hepáticas e somente em G. brasiliensis foi observado pâncreas exócrino intrahepático. O baço, em todas as espécies, foi caracterizado pela polpa vermelha e branca sem limites entre as duas regiões, mas somente em H. francisci foi registrado uma organização nodular no parênquima esplênico. G. brasiliensis e H. aff. malabaricus apresentaram na polpa branca CMMs associados principalmente a elipsoides. Além disso, nós observamos CMMs grandes no baço em relação ao fígado de G. brasiliensis e de H. aff. malabaricus. No fígado, valores altos de fibras reticulares e colágeno foram observado em G. brasiliensis. No baço, valores altos de fibras reticulares e colágeno foram registrados em H. aff. malabaricus e H. francisci, respectivamente. Diferenças histológicas confirmam a hipótese que a distância filogenética está refletida no fígado e no baço. Palavras-chave:...

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative histology in the liver and spleen of three species of freshwater teleost

et al. 2017

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Comparisons of Stereological and Other Approaches for Quantifying Macrophage Aggregates in Piscine Spleens

2019

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Macrophage aggregates (MAs) are focal accumulations of pigmented macrophages in the spleen and other tissues of fish. A central role of MAs is the clearance and destruction of degenerating cells and recycling of some cellular components. Macrophage aggregates also respond to chemical contaminants and infectious agents and may play a role in the adaptive immune response. Tissue damage or physiological stress can result in increased MA accumulation. As a result, MAs may be sensitive biomarkers of environmental stress in fish. Abundance of MAs in tissues has been reported in a variety of ways—most commonly as density, mean size, and relative area—but the utility of these estimates has not been compared. In this study, four different types of splenic MA abundance estimates (abundance score, density, relative area, and total volume) were compared in two fish populations (Striped Bass Morone saxatilis and White Perch M. americana) with a wide range in ages. Stereological estimates of total volume indicated an increase in MA abundance with spleen volume, which generally corresponded to fish age, and with splenic infections (mycobacteria or trematode parasites). Abundance scores were generally limited in the ability to detect changes in MA abundance by these factors, whereas density estimates were greatly influenced by changes in spleen volume. In some instances, densities declined while the total volume of MAs and spleen volume increased. Experimentally induced acute stress resulted in a decrease in spleen volume and an increase in MA density, although the total volume of MAs remained unchanged. Relative area estimates accounted for the size and number of MAs but not for changes in organ volume. Total volume is an absolute measure of MA abundance irrespective of changes in organ volume or patterns of accumulation and may provide an improved means of quantifying MAs in the spleens of fish.

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Pigmented macrophages and related aggregates in the spleen of european sea bass dosed with heavy metals: Ultrastructure and explorative morphometric analysis

Manera

Dezfuli

DePasquale

et al. 2018

Microscopy Res & Technique

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The ultrastructure and morphometrics of pigmented macrophages (PMs) were assessed in the spleen of European sea bass experimentally dosed with Cd and Hg. PMs occurred either as solitary cells or as variably structured aggregations, defined as macrophage aggregates (MAs). Light microscopy revealed a high degree of morphological heterogeneity amongst MAs of all experimental groups. At the ultrastructural level, MAs showed a heterogeneous pigment content that was not influenced by the treatment. Cytoplasm rarefaction/vacuolation and euchromatic nuclei, were observed in PMs of dosed fish. Undosed and Cd-dosed samples differ significantly with regard to the following morphometric features: the Minor axis of the best fitting ellipse, Aspect Ratio, and Roundness. In Cd-dosed fish, MAs showed reduced size and complexity. Lacunarity showed significant differences between undosed and both Cd and Hg-dosed samples. These results suggest that heavy metals, and especially Cd, may influence the dynamics of PM aggregation/disaggregation. Variability in splenic MAs was observed both by light and electron microscopy. However, only the morphometric techniques adequately and objectively described the phenomenon, allowing a quantitative/statistical comparison of morphology among experimental groups. These morphometric analyses could be usefully applied in toxicological and ecotoxicological, as well as morpho-functional studies.

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Characteristics of melanomacrophage centers in the liver and spleen of the roach Rutilus rutilus (Cypriniformes: Cyprinidae) in Lake Kotokel during the Haff disease outbreak

Cited by 19 publications

References 34 publications

Comparative histology in the liver and spleen of three species of freshwater teleost

Comparative histology in the liver and spleen of three species of freshwater teleost

Comparisons of Stereological and Other Approaches for Quantifying Macrophage Aggregates in Piscine Spleens

Pigmented macrophages and related aggregates in the spleen of european sea bass dosed with heavy metals: Ultrastructure and explorative morphometric analysis

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