Characteristics of interleukin-4 gene (C-589T, rs2243250) polymorphism in children with bronchial asthma and allergic rhinitis with isolated or allergic rhinitis-induced comorbid malocclusion

Shumna, Т. Ye.; Fedosieieva, O. S.; Zinchenko, Т. P.; Nedelska, S.M.; Voznyi, O. V.; Kamyshnyi, О. М.

doi:10.14739/2310-1210.2019.6.186484

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Shumna et al analyzed the relationship of the IL-4 T589C polymorphism with bronchial asthma, allergic rhinitis, and orthodontic pathology and concluded that the CC genotype of the IL-4 gene is associated with bronchial asthma (OR = 4.31; 95% CI = 1.63–11.36; p = 0.002) and allergic rhinitis (OR = 4.32; 95% CI = 1.04–7.81; p = 0.04) [ 32 ]. The TT genotype showed a predisposition to the development of orthodontic pathology.…”

Section: Discussionmentioning

confidence: 99%

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

Matsyura

Беш

Kens

et al. 2022

Life

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Cow’s milk allergy (CMA) is one of the most frequent types of food allergy. The aim of the study was to investigate whether IL-13 R130Q and IL-4 T589C polymorphisms are associated with the risk of CMA in young Ukrainian children. A total of 120 children (age range: 1–3 years) participated in the study and were divided into two groups: CMA (n = 60) and healthy controls (CNT, n = 60). The CMA group had two subgroups: specific oral tolerance induction (SOTI, n = 30) and milk elimination diet (MED, n = 30). The CNT group had two subgroups: positive family history of allergy (+FHA, n = 24) and negative family history of allergy (−FHA, n = 36). In the CMA group, molecular genetic testing of CC, CT, and TT genotypes of single nucleotide IL-4 T589C gene polymorphisms showed significantly higher rates of the CC genotype compared to healthy controls (92.2% vs. 58.8%; p < 0.01). In the CMA group, molecular genetic testing of GG, GA, and AA genotypes of single nucleotide IL-13 R130Q gene polymorphisms showed significantly higher rates of GA and AA polymorphic locus genotypes compared to healthy controls (43.5% vs. 22.4%, p < 0.05 and 8.7% vs. 0%, p < 0.05, respectively). In future studies, the genotypic and allelic distribution of these polymorphic variants will be determined in children with CMA and healthy children.

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Section: Discussionmentioning

confidence: 99%

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

Matsyura

Беш

Kens

et al. 2022

Life

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“…25, № 3 нетическую предрасположенность, нарушение регуляции и синтеза цитокинов [1,2,4,5,6,7,9,10]. Бронхиальная астма относится к многофакторным воспалительным заболеваниям, имеет сложную этиологию, зависящую от множества генетических факторов, контроль которого требует понимания различных механизмов иммунопатогенеза [3,8,11,12,13,14]. Мутации в генах цитокинов, определяющие дефекты продукции и рецепции отдельных цитокинов, составляют значительную часть иммуноопосредованных механизмов развития и прогрессирования патологических процессов при аллергических заболеваниях [1].…”

Section: генотипы цитокинов у детей с бронхиальной астмойunclassified

Association of gene polymorphism and cytokine content in the blood serum in children with allergic bronchial asthma

Просекова

Dolgopolov

Turyanskaya

et al. 2022

Russian Journal of Immunology

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The study of genes controlling cytokine activities of is among important tasks when assessing predisposition and revealing pathogenetic links of initiation and course of clinical disorders. Aberrant production of cytokines and dysregulation of immune response may be considered genetic predictors associated with differentiation and functioning of T helpers, being of decisive importance in pathogenesis of pediatric allergic bronchial asthma. Our objective was to evaluation of associations between polymorphic genotypes and serum levels of cytokines of various T helper profiles in the children with allergic bronchial asthma. We have observed 175 children aged 3 to 11 years. Of them, we have examined 75 patients diagnosed with allergic bronchial asthma (ABA) as well as 100 healthy children matched for age and gender. All children underwent general clinical and allergological examination. The contents of cytokines attributed to Th1, Th2 and Th17 profiles were determined in blood serum by means of ELISA technique. DNA samples isolated from peripheral venous blood were used for molecular genetic analysis. Using allele-specific PCR technique, the following mutation points were investigated: IFN (T-874 A), IL-4 (C-589 T), IL-6 (C-174 G), IL-17A (G- 197 A), TNF (G-308 A). The analysis of distribution and occurrence of the cytokine gene polymorphisms was carried out, and the odds ratio of the disease risk were calculated. Statistical data processing was carried out using the program Statistica 10 by methods of descriptive, parametric and non-parametric statistics, comparison of unrelated groups was performed by qualitative characteristics of HardyWeinberg equilibrium, and with Chi-square test ( 2). These studies have revealed differences in patterns and occurrence of polymorphic genotypes associated with aberrant production of cytokines typical for various Th profiles among the children with allergic bronchial asthma. A comparative analysis of the mutant allele frequencies and cytokine genotypes of various Th profiles, along with determination of the cytokine contents in blood serum of children with allergic bronchial asthma revealed a predominance of homozygous IFN 874A, IL-4 589T, IL-6 174G, IL-17A 197A, and TNF 308A genotypes. Studies of gene polymorphisms, features of production and content of the cytokines specific for T helpers 1, T helpers 2, T helpers 17 profiles in bronchial asthma in the children revealed differences in distribution and occurrence of mutant alleles associated with aberrant cytokine production, variable risk of developing allergic pathology and development of the distinct disease phenotype.

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Characteristics of Cytokine Gene Polymorphisms in Children With Different Phenotypes of Bronchial Asthma.

Просекова¹,

Dolgopolov

Сабыныч

et al. 2022

Russian Journal of Allergy

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Abstract BACKGROUND: Bronchial asthma is a chronic inflammatory disease of the airways, the development of which is based on genetic predictors associated with the differentiation and functioning of T-helpers. Polymorphisms in the genes of cytokines involved in the regulation of the direction of the T-helper mediated immune response are risk factors for the development of the disease and the realization of various phenotypes of bronchial asthma. AIMS: Study of the structure and frequency of occurrence of single nucleotide polymorphisms of cytokine genes with an assessment of the risk of various phenotypes of bronchial asthma in children. MATERIALS AND METHODS: In this case control study, 250 children were examined, including 150 children with a verified diagnosis of bronchial asthma (including 75 children with virus-induced and 75 children with allergen-induced disease phenotypes) and 100 sexually comparable healthy peers. The children underwent a comprehensive general clinical and allergological examination, genotyping, analysis of the structure, frequency of occurrence of cytokine gene polymorphisms and calculation of the odds ratio of the risk of developing different bronchial asthma phenotypes. DNA samples isolated from peripheral venous blood were used as material for molecular genetic analysis. The following mutation points were selected: IFN (T-874 A), IL-4 (C-589 T), IL-6 (C-174 G), IL-17A (G-197 A), TNF (G-308 A). The work followed the ethical principles set forth by the Declaration of Helsinki by the World Medical Association and the Rules of Clinical Practice in the Russian Federation, the study design was approved by the Interdisciplinary Ethics Committee of the Federal State Budgetary Educational Institution of Higher Education Pacific State Medical University of the Ministry of Healthcare of the Russian Federation on April 27, 2015 (Protocol No. 8), informed voluntary consent was signed by the parents. When processing digital data, we used the methods of descriptive, parametric and nonparametric statistics of the Statistica 10 program, comparison of unrelated groups by qualitative characteristics, assessment of the correspondence of the distributions of genotypes to the expected values at the Hardy-Weinberg equilibrium. The analysis of the frequency distributions of genotypes and alleles in two subpopulations was carried out using the Chi-square test (2). RESULTS: A comparative analysis of the frequencies of alleles and genotypes of cytokines of various Th profiles with the definition of features in allergen-induced and virus-induced phenotypes of the disease revealed in children with bronchial asthma the predominance of homozygous genotypes IFN (A-874A), IL-4 (T-589T), IL- 6 (G-174G), IL-17A (A-197A), TNF (A-308A), and in healthy peers the prevalence is IFN (T-874T), IL-4 (C-589C), IL-6 (C -174C), IL-17A (G-197G), TNF G-308G. Heterozygous genotypes IL-4 (C -589T), IL-6 (G -174C), IL-17A (G -197A), TNF (G -308A) found in children with bronchial asthma more often than in healthy peers, with the exception of the IFN genotype (T -874A). In children with the virus-induced bronchial asthma phenotype, the presence of the IL-4 (C-589T) mutant allele was found in 30,67% of cases with an odds ratio of -19,3 CI 95%. (11,23-33,31). When carrying the mutant A genotype IFN (T-874A), the odds ratio of the risk of developing the disease reflected a high degree of probability of the virus-induced bronchial asthma phenotype (OR = 5,11 (3,18-8,23) CI 95%). Carriage of homozygous genotypes IL-6 (G-174G), IL-17A (A-197A) determined an increased risk of developing allergen-induced bronchial asthma (OR = 2,71 (1,73-4,18) CI 95% and OR = 0,51 (0,32-0,71) CI 95%, respectively). Among children with bronchial asthma, there was a statistically significant increase in the incidence of the functionally unfavorable genotype 308 A /A of the TNF 308G /A gene, and the odds ratio reflects a 2,6-fold increase in the risk of developing a virus-induced bronchial asthma phenotype (2 = 18,66; p = 0,017; OR = 2,60, CI 95% (1,67-4,01). CONCLUSIONS: As a result of the study, significant differences were determined in the structure and frequency of occurrence of cytokine gene polymorphisms in children with allergen and virus-induced bronchial asthma, depending on the realized phenotype of the disease. Carriage of mutant alleles IFN (A-874A), IL-4 (T-589T), IL-6 (G-174G), IL-17A (A-197A), TNF (A-308A) can be characterized as genetic predictors of bronchial asthma, for the implementation of the virus-induced phenotype, the odds ratio is higher in the presence of mutant alleles IFN (A-874A), IL-4 (T-589T), TNF (A-308A), for the allergen-induced phenotype of the disease - IL-6 (G -174G), IL-17A (A-197A).

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Characteristics of interleukin-4 gene (C-589T, rs2243250) polymorphism in children with bronchial asthma and allergic rhinitis with isolated or allergic rhinitis-induced comorbid malocclusion

Cited by 3 publications

References 13 publications

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

Polymorphic Variants of Interleukin-13 R130Q and Interleukin-4 T589C in Children with and without Cow’s Milk Allergy

Association of gene polymorphism and cytokine content in the blood serum in children with allergic bronchial asthma

Characteristics of Cytokine Gene Polymorphisms in Children With Different Phenotypes of Bronchial Asthma.

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