Characteristics of human neural stem cells In Vitro and after transplantation into rat brain

Александрова, М. А.; Podgornyi, O. V.; Marei, M. V.; Полтавцева, Р. А.; Tsitrin, E. B.; Gulyaev, D. V.; Cherkasova, L V; Revishchin, A. V.; Korochkin, L. I.; Khrushchov, N. G.; Sukhikh, G. N.

doi:10.1007/s10517-005-0227-0

Cited by 7 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neurons are the main functional neural cells in the central nervous system (CNS). Knowing that cellular or gene therapy of CNS diseases needs large amounts of neurons differentiated from NSCs (Aleksandrova et al, 2005), the main purpose of our cell transplant experiment was to obtain neurons with related phenotypes and use them to promote the repair of CNS injuries. However, the local microenvironment in the injured area may limit the proliferation and differentiation of NSCs, resulting in a relatively low percentage of differentiation when NSCs are transplanted alone (Haughey et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

Chen

Cai

Shen

et al. 2014

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Neurotrophic factors can promote the proliferation and differentiation of neural stem cells (NSCs). Here we report that the possibility of using bFGF in combination with BDNF and NGF to promote proliferation and differentiation of NSCs in vitro. C57BL/6 mouse NSCs were cultured, passaged and stained by immunofluorescence for nestin and GFP. According to different neurotrophic factors added to NSCs, seven experiment groups (NGF, BDNF, bFGF, bFGF+NGF, bFGF+BDNF, NGF+BDNF and NGF+BDNF+bFGF) and a blank control group were established. One week after induction and differentiation, results showed that there was significant difference in the percentage of NSCs differentiating into neurons among the experiment groups. The percentage in the multi-factor groups was significantly higher than that in the single-factor groups (p<0.05), among which the percentage was the highest in NGF+BDNF+bFGF group. In the two-factor groups, the percentage in bFGF+NGF and bFGF+BDNF groups was significantly higher than that in NGF+BDNF group (p<0.05). The NSCs growth curves showed that cells proliferated continuously with the time of culture prolonging, but there was significant difference between the group containing bFGF and that without bFGF. Our results demonstrate that combined use of NGF/BDNF/bFGF significantly improved the ability of NSCs proliferation and differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

Chen

Cai

Shen

et al. 2014

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

show abstract

“…After xenotransplantation of human fetal neural stem cells to adult rat brain, their active migration was detected, which could promote repair of defects in neurodegenerative diseases [8,[16][17][18][19][20][21]. Note that the genetic constitution of the transplanted material should be taken into account, as it is known that migration and differentiation of neural stem cells is controlled by sets of special genes [22].…”

Section: Stem Cells As An Experimental Model For Analysis Of Gene Expmentioning

confidence: 99%

10.1007/s11177-008-3001-3

2010

CrossRef Listing of Deleted DOIs

View full text Add to dashboard Cite

The evidence obtained in the last 15 years has shed new light on the functioning of the brain tissue in norm and pathology. It has been shown that proliferating stem cells exist in the adult brain. Under certain conditions, these cells can participate in posttraumatic repair, replacing perished cells. The involvement of stem cells in the development of malignant tumors have been established. Numerous genomic mechanisms of regulating self-renewal of neural stem cells, their proliferation and differentiation have been found. These findings open new avenues in studying brain functions and development. They are used for designing cardinally novel technologies for treating neurogenerative diseases and brain cancers. In this review, we present new evidence on the genomic mechanisms involved in governing the fate of neural stem cells in vivo and in vitro.

show abstract

“…These cells are affected by various factors. It is known that culturing conditions determine NSPC maintenance, specifi city of differentiation of their descendants, and effectiveness of cells after their transplantation into the brain [1,25,28,37,40]. For successful maintenance of the primary cultures of human NSPC, the terms of material isolation, media, and culturing conditions should be selected.…”

mentioning

confidence: 99%

Analysis of Neural Stem Cells from Human Cortical Brain Structures In Vitro

et al. 2016

Self Cite

View full text Add to dashboard Cite

Comparative immunohistochemical analysis of the neocortex from human fetuses showed that neural stem and progenitor cells are present in the brain throughout the gestation period, at least from week 8 through 26. At the same time, neural stem cells from the first and second trimester fetuses differed by the distribution, morphology, growth, and quantity. Immunocytochemical analysis of neural stem cells derived from fetuses at different gestation terms and cultured under different conditions showed their differentiation capacity. Detailed analysis of neural stem cell populations derived from fetuses on gestation weeks 8-9, 18-20, and 26 expressing Lex/SSEA1 was performed.

show abstract

Characteristics of human neural stem cells In Vitro and after transplantation into rat brain

Cited by 7 publications

References 10 publications

Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

10.1007/s11177-008-3001-3

Analysis of Neural Stem Cells from Human Cortical Brain Structures In Vitro

Contact Info

Product

Resources

About