Analysis of Neural Stem Cells from Human Cortical Brain Structures In Vitro

Abstract

Comparative immunohistochemical analysis of the neocortex from human fetuses showed that neural stem and progenitor cells are present in the brain throughout the gestation period, at least from week 8 through 26. At the same time, neural stem cells from the first and second trimester fetuses differed by the distribution, morphology, growth, and quantity. Immunocytochemical analysis of neural stem cells derived from fetuses at different gestation terms and cultured under different conditions showed their differentiation capacity. Detailed analysis of neural stem cell populations derived from fetuses on gestation weeks 8-9, 18-20, and 26 expressing Lex/SSEA1 was performed.

Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells

“…Here, as well as in our earlier publication we showed that NSPCs maintained in FSC-containing medium supposed to promote astrocytic diffentiation, still capable of expressing such "stemness" markers as nestin and Lex/SSEA1, as well as an early marker of neuronal differentiation βIII-tubulin. Their expression is mostly localized in neurosphere-like floating cell aggregates [21] . Probably, maintaining of NSPCs cultures in serum-containing media results in the accumulation of the least differentiated cells in neurospheres, while more differentiated cells adhere to cultural plastic and are eliminated at passaging.…”

Thousand and one kinase 1 protects MCAO-induced cerebral ischemic stroke in rats by decreasing apoptosis and pro-inflammatory factors

“…The primary neural stem cells were obtained from the cerebral cortex of embryo at 18 days gestation rats as described previously [20]. In brief, the cerebral cortices were digested with 0.25% trypsin, and then the cell suspension was seeded into six-well plates pre-coated with poly- l -lysine.…”