Characteristics of human lipoaspirate-isolated mesenchymal stromal cells cultivated under lower oxygen tension

Буравкова, Л. Б.; Grinakovskaya, O. S.; Andreeva, E. R.; Zhambalova, A. P.; Kozionova, M. P.

doi:10.1134/s1990519x09010039

Cited by 61 publications

(51 citation statements)

References 14 publications

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“…The proliferation of mesenchymal stem cells is increased under hypoxic conditions as compared to normoxic conditions (3,7). In addition, hypoxia amplifies the paracrine effects of mesenchymal stem cells by enhancing the secretion of certain growth factors.…”

Section: Discussionmentioning

confidence: 89%

Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion

Park

Kim

Choi³

et al. 2010

Biomed. Res.

151

118

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Adipose-derived stem cells (ADSCs) and their secretomes mediate diverse skin-regeneration effects, such as wound-healing and antioxidant protection, that are enhanced by hypoxia. We investigated the hair-growth-promoting effect of conditioned medium (CM) of ADSCs to determine if ADSCs and their secretomes regenerate hair and if hypoxia enhances hair regeneration. If so, we wanted to identify the factors responsible for hypoxia-enhanced hair-regeneration. We found that ADSC-CM administrated subcutaneously induced the anagen phase and increased hair regeneration in C 3 H/NeH mice. In addition, ADSC-CM increased the proliferation of human follicle dermal papilla cells (HFDPCs) and human epithelial keratinocytes (HEKs), which are derived from two major cell types present in hair follicles. We investigated the effect of hypoxia on ADSC function using the same animal model in which hypoxia increased hair regrowth. Forty-one growth factors in ADSC-CM from cells cultured under hypoxic or normoxic conditions were analyzed. The secretion of insulin-like growth factor binding protein (IGFBP)-1, IGFBP-2, macrophage colony-stimulating factor (M-CSF), M-CSF receptor, platelet-derived growth factor receptor-β, and vascular endothelial growth factor was significantly increased by hypoxia, while the secretion of epithelial growth factor production was decreased. It is reasonable to conclude that ADSCs promote hair growth via a paracrine mechanism that is enhanced by hypoxia.

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Section: Discussionmentioning

confidence: 89%

Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion

Park

Kim

Choi³

et al. 2010

Biomed. Res.

151

118

View full text Add to dashboard Cite

show abstract

“…However, there are no studies to-date investigating the effect of oxygen levels on hMSCs and expression of tendon lineage markers, as the literature currently focuses on bone and cartilage, with results remaining inconclusive 17,21,32 . Lower oxygen tension with hMSC culture has been shown in a number of investigations to maintain their progenitor state, and appears to regulate their proliferation, viability, and their commitment to differentiation 17,23,24 . Malla- …”

Section: Discussionmentioning

confidence: 99%

Learning about PRP using cell-based models

Beitzel

McCarthy

Russell

et al. 2014

MLTJ

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SummaryIntroduction: cell-based tissue engineering strategies using human mesenchymal stem cells (hMSCs) may help to augment tendon healing. To further investigate the in-vitro behavior of this cell population, we investigated low oxygen culture levels, and growth factor supplementation and their effect on expression of tendon extracellular proteins and cell proliferation. Materials and methods: bone marrow aspirate (BMA) was harvested during arthroscopic rotator cuff repair. Characterized hMSCs derived from BMA were incorporated into 3-dimensional tissue engineered constructs (TECs). TECs were analyzed by frozen sections with immunohistochemistry for cell density, collagen I and collagen III expression. Results: growth factor stimulation and low oxygen increased cell density within TECs. Low oxygen and addition of growth factors to culture media demonstrated an increase in collagen I and III expression, both in ambient oxygen conditions and low oxygen conditions. Conclusion: low oxygen and TGFβ3 demonstrated a positive effect on cell number, and type I and III collagen expression in 3D culture environments.

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“…Буравковой и соавт. [17]. Ткань измельчали и обрабатывали 0,075% раствором кол-лагеназы IA в среде α-МЕМ в течение 35 мин при 37 °С.…”

Section: дизайн исследованияunclassified

The effects of cysplatin on human adipose tissue derived mesenchymal stromal cells under different oxygen levels

2016

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Characteristics of human lipoaspirate-isolated mesenchymal stromal cells cultivated under lower oxygen tension

Cited by 61 publications

References 14 publications

Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion

Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion

Learning about PRP using cell-based models

The effects of cysplatin on human adipose tissue derived mesenchymal stromal cells under different oxygen levels

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