Characteristics of cerebral non‐histone chromatin proteins as revealed by polyacrylamide gel electrophoresis

The characterization of the non-histone chromosomal proteins of the main classes of nuclei from rat brain fractionated by zonal centrifugation

“…The combination of the two electrophoretic systems in the two-dimensional electrophoresis, apart from giving a clearer picture of the complexity and heterogeneity of non-histone chromosomal proteins, underlined the limited specificity of patterns obtained by the other two systems. Limited specificity of brain non-histone chromosomal proteins was reported by other authors (Olpe et al, 1973;Tashiro et al, 1974), whereas Dravid & Burdman (1968) could not detect any differences in the proteins from neurons, astrocytes and oligodendrocytes and Panchenko et al (1974) could detect no differences in the proteins derived from four brain regions. Limited tissue and species specificity of non-histone chromosomal proteins was shown in a number of different species and tissues (Elgin & Bonner, 1970;MacGillivray et al, 1971MacGillivray et al, , 1972Wu et al, 1973).…”

“…On the other hand Dravid & Burdman (1968) fractionated nuclei with the technique of L0vtrup- Rein & McEwen (1966) and they could not show any electrophoretic differences between soluble acidic proteins isolated from the nuclei of neurons, astrocytes and oligodendrocytes. Tashiro et al (1974) fractionated nuclei from cerebral cortex into two fractions: neuron-rich and oligodendroglial nuclei. They claim the existence of a group of high-molecular-weight non-histone proteins in neuronal chromatin that is apparently absent from oligodendroglial chromatin.…”

Comparison of the number of RNA initiation sites in rat brain fractions enriched in neuronal or glial nuclei

“…More recent studies on the initiation of RNA synthesis support the view that chromatin proteins are involved in the selection of initiation sites for RNA polymerases [5,6]. Potentially the different rates of phosphorylation [7] and acetylation [8] of chromatinbound neuronal and glial proteins as well as the differences in chromatin composition between both nuclear fractions [7,9] may be involved in the regulation of neuronal and glial transcription.…”

A comparative study of histone acetylation in neuronal and glial nuclei enriched rat brain fractions

“…Neuronal chromatin ternplated transcription is much less restricted than that of oligodendro-and microglia [ 1,2] . These differences in chromatin template restriction are connected with differences in chromatin composition [3,4] and structure [S,lO] and may be related to different enzymatic modifications of chromosomal proteins such as the previously described higher phosphorylation of neuronal nonhistone proteins [4].…”