Characteristics of catechin extracted from cocoa husks using microwave assisted extraction (MAE)

Abstract

Abstract. Rahayu PP, Rosyidi D, Purwadi, Thohari I. 2019. Characteristics of catechin extracted from cocoa husks using microwave-assisted extraction (MAE). Biodiversitas 20: 3626-3631. Cocoa husks, a by-product of cocoa processing, were investigated as a source of catechin. The objective of this research was to investigate the best extraction time for catechin of cocoa husk using microwave-assisted extraction (MAE). Four different treatments (4, 6, 8, and 10 min) were tested. Catechin was analyzed using Fourier transform infrared (FTIR) spectroscopy, and total phenol content (TPC) and total catechin content (TCC) were determined by liquid chromatography-mass spectrometry (LC-MS). The longest extraction time (10 min) produced the highest phenolic content, although it was similar to that obtained at 8 min. The IR spectra of the cocoa husk extract were stable for the 4, 6, and 8 min extractions. However, the spectra after the 10 min extraction showed more peaks, especially for phenolic compounds and catechins. The mass spectra of catechin showed a peak at RT 3.11 and a protonated ion peak [M+H]+ at m/z 307. The results for TPC, TCC, FTIR, and LC-MS indicated that a 10 min extraction using MAE was the optimum treatment.

Perbandingan efektifitas pretreatment ozonolisis dan hydrothermal pressure dalam meningkatkan kandungan tanin dan kafein ekstrak kulit biji kakao (Theobroma cacao L.)

“…Proses pengolahan biji kakao menghasilkan limbah berupa kulit biji kakao, yang jika tidak dikelola dengan baik dapat menyebabkan dampak lingkungan dan ekonomi yang signifikan. Kulit biji kakao adalah produk sampingan utama dari industri cokelat, dipisahkan dari kotiledon selama proses pra-panggangan atau setelah proses panggang (Rosyidi et al, 2019). Menurut Kim et al (2004), persentase kulit biji kakao terhadap total berat biji kakao kering mencapai 10-12%, sehingga peningkatan produksi kakao berpotensi mengakibatkan peningkatan limbah kulit biji kakao.…”

Enhancing bioactive content in Malus sylvestris peel extract for antioxidant source using microwave-assisted extraction (MAE) and predicting the potential of ant nests

“…Malus sylvestris extraction was conducted using the MAE method 17 with a slight modification, employing a Sharp Model R -222Y(S) microwave at a medium (50°C). The Malus sylvestris powder was mixed with 50% methanol at a ratio of 5 grams of powder to 100 milliliters of methanol and allowed to soak for 24 hours.…”

Characterization of Andrographis Paniculata Extract Obtained by Microwave-Assisted Extraction (MAE) Method with Radiation Time

“…The AP was extracted by the MAE method (Rahayu et al, 2019)(Sharp Model R -222Y(S)) at a level power high (70°C) with a slight modification. AP powder was dissolved in methanol 50% at a ratio of 5 g powder to 100 mL methanol.…”