Characteristics of Bordetella hinzii strains isolated from a cystic fibrosis patient over a 3-year period

Funke, Guido; Heß, Thomas; Graevenitz, A. von; Vandamme, Peter

doi:10.1128/jcm.34.4.966-969.1996

Cited by 82 publications

(32 citation statements)

References 15 publications

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“…By 16S rRNA sequence analysis B. hinzii clusters with the bordetellae and is most closely related to B. bronchiseptica and B. parapertussis. Our phylogenetic data thus confirm the taxonomic position of B. hinzii and corroborate the findings of others on the basis of 23S rRNA-DNA hybridization and whole-cell protein analysis (7,18).…”

Section: Resultssupporting

confidence: 90%

“…The second strain was identified from the sputum of a patient in France, but further details were not available (18). The third and fourth strains were obtained from cultures of consecutive sputum samples from a cystic fibrosis patient over a 3-year period (7). In most of these sputum sample cultures, B. hinzii was associated with other respiratory pathogens, but in some cultures, B. hinzii was the sole organism isolated.…”

Section: Resultsmentioning

confidence: 99%

“…The proportions of some CFAs in B. hinzii vary depending on whether bacterial cells are harvested after 24 or 48 h of incubation (3,7,18). The CFA profile of B. hinzii when it was harvested after 24 h of incubation discriminated between B. hinzii and the phenotypically related species B. avium in previous studies (3,7). However, as noted previously by Vandamme et al (18), we found that the CFA profiles of B. hinzii and B. avium are indistinguishable after 48 h of culture (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Lastly, Bordetella hinzii colonizes the respiratory tracts of poultry (18). Five strains of B. hinzii have so far been isolated from human sources (3,7,17,18), and three of these strains have been documented to cause infection (3,7). We report on the first human infection with B. hinzii that resulted in fatal septicemia.…”

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Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

Kattar¹,

Chávez

Limaye

et al. 2000

J Clin Microbiol

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We report on the first case of fatal septicemia caused byBordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella aviumwith three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

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Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

Kattar¹,

Chávez

Limaye

et al. 2000

J Clin Microbiol

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“…They are found only in warm‐blooded animals. Bordetella pertussis and Bordetella parapertussis are human respiratory tract pathogens, Bordetella bronchiseptica and Bordetella hinzii are animal pathogens but are increasingly found in AIDS and cystic fibrosis patients [1–4]. Whereas B. pertussis produces R‐type lipopolysaccharide (LPS) without the polysaccharide O‐chain, B. bronchiseptica and B. hinzii LPSs have O‐chains.…”

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The structure of the carbohydrate backbone of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica

Vinogradov

2000

European Journal of Biochemistry

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The structure of the core-lipid A region of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica has been analyzed. Lipopolysaccharides were deacylated using strong alkaline hydrolysis, the products were separated by high performance anion-exchange chromatography and analyzed by NMR and mass spectrometry. The following structure of the products can be deduced from the experimental results: Bacteria of the genus Bordetella are gram-negative aerobic coccobacilli, assigned to the family Alcaligenaceae. They are found only in warm-blooded animals. Bordetella pertussis and Bordetella parapertussis are human respiratory tract pathogens, Bordetella bronchiseptica and Bordetella hinzii are animal pathogens but are increasingly found in AIDS and cystic fibrosis patients [1±4]. Whereas B. pertussis produces R-type lipopolysaccharide (LPS) without the polysaccharide O-chain, B. bronchiseptica and B. hinzii LPSs have O-chains. B. parapertussis and B. bronchiseptica produce an O-chain with the same repeating unit [5]. The structure of the core part of Bordetella LPS has been partially determined only for B. pertussis [6]. Here we present the structure of the carbohydrate backbone of the LPS from B. bronchiseptica and B. hinzii. E X P E R I M E N T A L P R O C E D U R E SBacterial strains and lipopolysaccharide isolation B. bronchiseptica strain 110H was obtained from D. Bemis (University of Tennessee, Knoxville, TN, USA) and was originally isolated from a dog in New York in 1970. B. bronchiseptica strain Bp512 (formerly NCTC 8762) was obtained from James Musser (NIH, NIAID, Rocky Mountain Labs, Hamilton, MT, USA) and was originally isolated from the upper respiratory tract of a human in Manchester, UK in 1950. B. hinzii strain ATCC 51730 was originally isolated from the blood of an AIDS patient. Cells were cultivated and LPS isolated as described previously [5].NMR spectroscopy and general methods 1 H and 13 C NMR spectra were recorded using a Varian Inova 500 spectrometer in D 2 O solutions at 25 8C with acetone standard (2.225 p.p.m. for 1 H and 31.5 p.p.m. for 13 C) using vnmr 6.1b software pulse sequences gDQCOSY, TOCSY (mixing time 120 ms), NOESY (mixing time 200 ms), gHSQC, gHMBC (optimized for 5 Hz coupling constant), gHSQC-TOCSY, digital resolution in F2 was 1.2 Hz´point 21X31 P and 1 H± 31 P HMQC (optimized for 7 Hz coupling constant) spectra were recorded on a Varian Inova 400 spectrometer. Spectra were assigned with the help of the pronto program [7]. Electrospray mass spectra were obtained using a Micromass Quattro spectrometer in 50% MeCN with 0.2% HCOOH at a flow rate of 15 mkl´min 21 with direct injection. GLC, GLC-MS, methylation and monosaccharide analyzes were performed as described previously [8]. Preparation of oligosaccharidesLPS from each bacterial strain (200 mg) was heated at 100 8C for 4 h in 4 m NaOH (4 mL) containing a small amount of NaBH 4 , cooled and neutralized to pH 8±9 with 2 m HCl (7.5 mL). The precipitate was removed by centrifugation and the products were separated...

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Structure of the lipid A of Bordetella hinzii ATCC 51730

Aussel

Brisson

Perry

et al. 2000

Rapid Commun. Mass Spectrom.

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Bordetella hinzii has recently been isolated from immunocompromised human hosts. The structure of the lipid A of its endotoxin was investigated using chemical analyses, nuclear magnetic resonnance (NMR), gas liquid chromatography/mass spectrometry (GC/MS), plasma desorption mass spectrometry (PDMS) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The lipid A contains the classical bisphosphorylated beta-(1-->6)-linked D-glucosamine disaccharide with hydroxytetradecanoic acid (C14OH) in amide linkages. The lipid A components of B. pertussis, B. bronchiseptica, and B. parapertussis all differ in their acylation pattern but share a residue of tetradecanoyl-3-hydroxytetradecanoic acid in amide linkage at the C-2' position. However, in the B. hinzii species, the tetradecanoic acid (C14) is stoichiometrically replaced by a 2-hydroxytetradecanoic acid (2-C14OH). In the few reported examples of a hydroxylated fatty acid in this position, the substitutions were only partial. The B. hinzii lipid A differs from that of B. pertussis also by replacement of the hydroxydecanoic acid (C10OH) by hydroxydodecanoic acid (C12OH) and by the presence of a hexadecanoic acid (C16) to give a sixth fatty acid. The lipid A was heterogeneous, being composed of three major molecular species: tetra-, penta- and hexaacylated. The fatty acids in ester linkage were localized by PDMS of the native and alkali-treated lipid A. The lipid A components isolated from the O-chain-linked lipopolysaccharides (LPSs) were shown to be more acylated than those from the O-chain-free LPSs.

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Characteristics of Bordetella hinzii strains isolated from a cystic fibrosis patient over a 3-year period

Cited by 82 publications

References 15 publications

Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

The structure of the carbohydrate backbone of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica

Structure of the lipid A of Bordetella hinzii ATCC 51730

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