Application of 16S rRNA Gene Sequencing To Identify
            <i>Bordetella hinzii</i>
            as the Causative Agent of Fatal Septicemia

Kattar, Mireille M.; Chávez, Juan francisco Hernández; Limaye, Ajit P.; Rassoulian-Barrett, Sara L.; Yarfitz, Stuart; Carlson, L. C.; Houze, Yolanda B.; Swanzy, Susan R.; Wood, Brent L.; Cookson, Bt

doi:10.1128/jcm.38.2.789-794.2000

Cited by 73 publications

(17 citation statements)

References 19 publications

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“…In cases where the genus and species could not be correctly identified with these methods, or when the results obtained by the MALDI-TOF MS and conventional methods were discordant, PCR and sequencing of the 16S rRNA gene were performed for definite identification, as described previously. 8 In addition, MALDI-TOF MS analyses were also performed on subcultured colonies derived from the positive blood culture bottles ('indirect' method) for comparison with the direct identification method.…”

Section: Reference Methods For Bacterial Identificationmentioning

confidence: 99%

Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

Yonetani

Ohnìshì

Ohkusu

et al. 2016

International Journal of Infectious Diseases

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Section: Reference Methods For Bacterial Identificationmentioning

confidence: 99%

Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

Yonetani

Ohnìshì

Ohkusu

et al. 2016

International Journal of Infectious Diseases

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“…The nucleotide sequence (1457 bp) of the 16S rRNA gene of strain 10d was 96.7% identical with that of Bordetella avium DSM 11334 T (accession no. AF177666), 96.7% identical with that of Bordetella hinzii DSM 4922 T (AF177667), and 96.0% identical with that of Bordetella bronchiseptica (AJ278452) [26,27]. This close phylogenic relatedness with other members of the genus Bordetella [27,28] was also reflected in the following characteristics of strain 10d: the DNA GC content was 67.0 mol%, the isoprenoid quinone Q-8 was detected, tetrazolium was reduced, nicotinamide was required for growth and potassium tellurite inhibited growth.…”

Section: Identification Of a 4-amino-3-hydroxybenzoate-assimilating Omentioning

confidence: 99%

A novel meta‐cleavage dioxygenase that cleaves a carboxyl‐group‐substituted 2‐aminophenol

Takenaka¹,

Asami

Orii

et al. 2002

European Journal of Biochemistry

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A bacterial strain that grew on 4-amino-3-hydroxybenzoic acid was isolated from farm soil. The isolate, strain 10d, was identified as a species of Bordetella. Cell extracts of Bordetella sp. strain 10d grown on 4-amino-3-hydroxybenzoic acid contained an enzyme that cleaved this substrate. The enzyme was purified to homogeneity with a 110-fold increase in specific activity. The purified enzyme was characterized as a meta-cleavage dioxygenase that catalyzed the ring fission between C2 and C3 of 4-amino-3-hydroxybenzoic acid, with the consumption of 1 mol of O 2 per mol of substrate. The enzyme was therefore designated as 4-amino-3-hydroxybenzoate 2,3-dioxygenase. The molecular mass of the native enzyme was 40 kDa based on gel filtration; the enzyme is composed of two identical 21-kDa subunits according to SDS/PAGE. The enzyme showed a high dioxygenase activity only for 4-amino-3-hydroxybenzoic acid. The K m and V max values for this substrate were 35 lM and 12 lmolAEmin, respectively. Of the 2-aminophenols tested, only 4-aminoresorcinol and 6-aminom-cresol inhibited the enzyme. The enzyme reported here differs from previously reported extradiol dioxygenases, including 2-aminophenol 1,6-dioxygenase, in molecular mass, subunit structure and catalytic properties.

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“…However, for most clinical bacterialisolates the initial 500-bp sequence provides adequate differentiation for identification and in fact can provide a bigger percent difference between strains because the region shows slightly more diversity per kilobase sequenced. Kattar et al (2001) found that 66% of the variability in the 16 S rRNA gene sequence among Bordetella species was in the first 500 bp. Evaluations published in the literature, made using the microseq database (AppliedBiosystems Inc. [ABI], Foster City, Calif.), are usually based on the 500-bp sequence (Tang et al, 1998;Patel et al, 2000;TangHall et al, 2000;Hall, et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Four novel strains of cellulolytic symbiotic bacteria isolated and characterized from GI tract of marine fishes of various feeding habits

Augustine

Imelda

2018

Biocatalysis and Agricultural Biotechnology

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Selected marine fishes with different feeding habits were screened for the presence of symbiotic cellulolytic bacteria in their gut. Four cellulolytic species of symbiotic bacteria were isolated from GI tract of marine fishes namely Carangoides praeustus, Filimanus similis, Sardinella longiceps and Sillago sihama. The strains were identified after polyphasic phenotypic and genotypic (16S rRNA gene) characterization as Bacillus subtilis strain TCPC1, Vibrio alginolyticus strain CFSS2C2, Pseudomonas stutzeri strain KSLS4C3 and Klebsiella oxytoca strain MSSC4. (Genbank Accession nos.: JN710380, JN710378, JN710377, JN712301).The results indicated the presence of cellulolytic bacteria in GI tract of marine fishes of carnivorous, phytoplanktivorous and omnivorous feeding habits. Cellulolytic activity was the maximum for B. subtilis strain TCPC1 (0.45 mg glucose ml −1) and V. alginolyticus strain CFSS2C2 (0.24 mg glucose ml −1) at 234 h. While P. stutzeri strain KSLS4C3 showed the maximum utilization (0.22 mg glucose ml −1) from 240 to 258 h. K. oxytoca strain MSSC4 (0.47 mg glucose ml −1) showed three peaks during the study. The maximum rate of cellulose utilization was shown by P. stutzeri strain KSLS4C3 (0.05 mg glucose ml −1 medium h −1) followed by K. oxytoca strain MSSC4 (0.03 mg glucose ml −1 medium h −1).

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Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

Cited by 73 publications

References 19 publications

Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

A novel meta‐cleavage dioxygenase that cleaves a carboxyl‐group‐substituted 2‐aminophenol

Four novel strains of cellulolytic symbiotic bacteria isolated and characterized from GI tract of marine fishes of various feeding habits

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