Characteristics of a retrovirus associated with Jembrana disease in Bali cattle

Kertayadnya, G.; Wilcox, G. E.; Soeharsono, S.; Hartaningsih, Nining; Coelen, R.J.; Cook, Rachelle; Collins, Margaret E.; Brownlie, Joe

doi:10.1099/0022-1317-74-9-1765

Cited by 49 publications

(43 citation statements)

References 14 publications

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“…Based on an early hypothesis that the disease had a rickettsial aetiology [11] and known role of arthropods in the transmission of many rickettsial diseases, arthropods have been suspected to be involved in its transmission, and insecticides have been used to assist in control of disease outbreaks (unpublished observations). The role of arthropods in the disease has, however, never been confirmed and the disease has now been shown to be caused by a lentivirus [4,9].…”

Section: Discussionmentioning

confidence: 99%

“…Infection of other bovine species induces only a mild or subclinical infection [3]. The disease is caused by Jembrana disease virus (JDV), a recently-described lentivirus that is antigenically and genetically related to, but distinguishable from, bovine immunodeficiency-like virus (BIV) [4,5]. The acute nature of Jembrana disease with recovery of most affected animals and no recurrence of disease in the recovered animals [6] is unusual for a lentivirus.…”

Section: Introductionmentioning

confidence: 99%

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The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Soeharsono¹,

Wilcox

Putra³

et al. 1995

Epidemiol. Infect.

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SummaryMethods of transmission of Jembrana disease, an acute and severe disease of Bali cattle (Bos javanicus) caused by a recently-identified bovine lentivirus known as Jembrana disease virus, are described. During the acute disease virus can be detected in saliva and milk. There is evidence of direct transmission from acutely affected animals in close contact with susceptible cattle, possibly by virus in these secretions infecting cattle by the conjunctiva!, intranasal or oral routes, by which it was possible to infect cattle experimentally. During the acute disease the titre of infectious virus in blood is high, about 108 50% cattle infectious units (ID50)/ml, and it is probable that the virus is also transmitted mechanically by haematophagous arthropods. Recovered cattle are also a potential but probably infrequent source of infection; recovered cattle are persistently viraemic but the titre of infectious virus in blood decreases to about 101 ID50/ml by 60 days after recovery from the acute disease, and virus cannot be detected in secretions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Soeharsono¹,

Wilcox

Putra³

et al. 1995

Epidemiol. Infect.

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“…Among these antigens, a 24 kDa protein cross-reacted with the 26 kDa capsid protein of bovine immunodeficiency virus (BIV) of the lentivirus subfamily. This analysis allowed to definitively affiliate JDV to the subfamily lentiviruses of the family Retroviridae (Kertayadnya et al, 1993;Wilcox et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…It was further established as a member of the family Retroviridae, due to the associated disease, the reverse transcription (RT) activity, the morphological characteristics, the virus maturation by C-type budding through the plasma membrane and into the intracytoplasmic vacuoles, formation of spherical virus particles of 96-124 nm in diameter with smooth membrane which is characteristic of enveloped viruses and frequently with an eccentric nucleoid observed under electron microscopy (Kertayadnya et al, 1993;Wilcox et al, 1992). Furthermore, Western immunoblotting using sera from recovered animals identified several proteins with sizes ranging from 14 kDa to 100 kDa.…”

Section: Introductionmentioning

confidence: 99%

The Structure and Function of Jembrana Disease Virus Genome

Kusumawati¹,

Wanahari²,

Tampubolon³

2014

J. Inf. Mol. Biol.

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“…The disease was endemic through-out parts of Indonesia, including Java, Sumatra [9], and also Australia [4]. Jembrana disease virus (JDV), belongs to the lentivirus family of retrovirus [2,11]. Genetically and antigenically, the most closely related to JDV is bovine immunodeficiency virus (BIV) although the respective associated disease is quite different [2].…”

Section: Introductionmentioning

confidence: 99%

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

et al. 2015

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Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on conserved tm subunit of Jembrana disease virus env gene. The RT-LAMP conditions were optimized by varying the concentration of MgSO 4 , betaine, dNTP, and temperature as well as the time and duration of reaction. The primers sensitivity for JDV was confirmed. The method was able to detect env-tm gene dilution which contained 2 9 10 -15 g of template. Comparatively, the sensitivity of RT-LAMP/LFD was 100-fold more sensitive than reverse transcription-polymerase chain reaction. The primers specificity for JDV was also confirmed using positive and negative controls. This work also showed that virus detection could be done not only on total RNA extracted from blood but various organs could also be analyzed for the presence of JDV using RT-LAMP/LFD method. The whole process, including the LAMP reaction and the LFD hybridization step only lasts approximately 75 min. Results of analysis can be easily observed with naked eyes without addition of any chemical or further analysis. The combination of RT-LAMP with LFD makes the method a more suitable diagnostic tool in conditions where sophisticated and expensive equipments are not available for field investigations on Jembrana disease in Bali cattle.

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Characteristics of a retrovirus associated with Jembrana disease in Bali cattle

Cited by 49 publications

References 14 publications

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

The Structure and Function of Jembrana Disease Virus Genome

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

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