“…The temperature of the surface layer of the manure pile was 29°C. The following 2-(4-[hydroxyethyl]-1-piperazinyl) ethanesulfonic acid (HEPES)-trace elements medium modified from HEPES medium ( 9 ) was used for the cultivation of strain KYUHI-S T . The medium was composed of HEPES (11.92 g L −1 , 50 mM), KH 2 PO 4 (0.5 g L −1 ), NaHCO 3 (0.5 g L −1 ), MgSO 4 ·7H 2 O (0.1 g L −1 ), (NH 4 ) 2 SO 4 (2.5 g L −1 , equivalent to 38 mM NH 4 + ), CaCl 2 ·2H 2 O (5 mg L −1 ), EDTA-Fe (III) (1 mg L −1 ), 5 mL of bromothymol blue solution (bromothymol blue 1 mg mL −1 , Na 2 HPO 4 945 mg mL −1 , and K 2 PO 4 0.49 mg mL −1 in deionized water), and 5 mL of trace elements (NaMoO 4 ·2H 2 O 10 mg, MnCl 2 ·4H 2 O 20 mg, CoCl 2 ·6H 2 O 0.2 mg, CuSO 4 ·5H 2 O 2 mg, ZnSO 4 ·7H 2 O 10 mg, FeSO 4 ·7H 2 O 770 mg, and EDTA·2Na 1.03 g in 1,000 mL of deionized water).…”