Characteristics and Mode of Growth of a Tissue Culture Strain (EB1) of Human Lymphoblasts From Burkitt's Lymphoma2

Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

“…Continuous suspension cultures of leukocytes may be derived from the peripheral blood of patients with lymphomas and leukemias and also from normal individuals [4,15,18,26,32]. The present report describes several of the properties of such cell lines cultured from normal individuals and those with cystinosis and illustrates the feasibility of utilizing these cells for investigations of the pathogenesis of cystinosis.…”

Analysis of Major Histocompatibility Complex Gene Products in Tissues Isolated from the Developing Human Nervous System

“…Total RNA isolated from human fetal DRG tissue was assayed for the presence of MHC class I-and II-specific RNA sequences and the level was compared to U937 cells (provided by Dr. Francisco Gonzalez-Scarano, The University of Pennsylvania, Phila delphia, Pa.), a human monocyte-derived cell line shown to be MHC class I protein positive, but class II-negative [25]; U-373 MG cells (provided by Dr. Francisco Gonzalez-Scarano), a human glioma cell line which has been shown to express a moderate amount of MHC class I protein on its cellu lar surface [26] and Raji cells, a B lympho cyte-derived cell line shown to express a sig nificant amount of class II protein on its cel lular surface [27] with no expression of MHC class I-specific protein. Total RNA Fig.…”

Tumor Antigens

“…Most studies concerning serum protein production by various tumors have employed the use of cell lines, the majority of which were composed of lymphoblastoid cells originating either from the peripheral blood of patients with leukemia [16][17][18][19][20][21][22] or from biopsies of tumors from patients with Burkitt's lymphoma [23][24][25][26][27][28][29][30] and more recently from a human reticulum cell sarcoma [31]. Except for a few Burkitt's Lymphoma lines [32,33] almost all of the lines which have been investigated thus far have been found to produce one or more immunoglobulins or subunits thereof [22,[33][34][35][36], Stech e r and T h o rbeck e [13] in comparing several tissue culture cell lines, characterized as fibroblastic, epithelial-like or lymphoblastic with regard to their abilities to form serum proteins in vitro, found that fibroblast-like cells of both human and murine origin produced scrum proteins associated with complement activity.…”