Characteristics and diversity of Rhizoctonia spp. population in soil of selected forest bare-root nurseries in Poland

Abstract

Fourty three <em>Rhizoctonia</em> isolates obtained from four forest nurseries situated in the Wielkopolska region (central-western Poland) has been proved as multinucleate (anamorph – <em>R. solani</em>). They represented four anastomosis groups (AG): AG1-IC, AG-5, AG4-HG2 and AG2-1. Three AGs were found in Jarocin nursery (AG-5, AG4-HG2 and AG2-1), two in Łopuchówko (AG-5 and AG4-HG2) and one in Konstantynowo (AG1-IC) and Pniewy (AG-5). All isolates were highly pathogenic to Scots pine (<em>Pinus sylvestris</em>) seedlings and pose a large damping-off threat to the seedlings in the nurseries with single AG and in those where more AGs exists.

Diversity of multinucleate Rhizoctonia spp. in soil of two forest nurseries Garncarskibród and Lipka

“…The research by Stępniewska-Jarosz et al (2006) and Bełka and Mańka (2014) were partially conducted in the same area giving different results. In the later work (Bełka and Mańka, 2014) isolates AG4-HG2 were observed. The difference may have resulted from possible bridging of some isolates belonging to certain AGs with isolates belonging to the other (Ogoshi, 1972) or from the fact that different techniques were used to assign isolates to certain AGs.…”

“…As different anastomosis groups have different sensitivity to fungicides (Carling et al, 1999;Campion et al, 2003) and are characterized by different pathogenicity (Bełka and Mańka, 2014), the knowledge of their occurrence in soil could have a big impact on seedling protection approach. The results of some studies (Herr, 1995;Sharon et al, 2011) suggest the possibility of using non-pathogenic Rhizoctonia strains as biological control agents against those highly pathogenic ones.…”

“…In Polish nurseries, so far only the research by Stępniewska-Jarosz et al (2006), and Bełka and Mańka (2014) described the differences between anastomosis groups in Polish forest nurseries. Stępniewska-Jarosz et al (2006) found five, and Bełka and Mańka (2014) isolated four different anastomosis groups from Polish forest nurseries soil. The research by Stępniewska-Jarosz et al (2006) and Bełka and Mańka (2014) were partially conducted in the same area giving different results.…”

“…Stępniewska-Jarosz et al (2006) found five, and Bełka and Mańka (2014) isolated four different anastomosis groups from Polish forest nurseries soil. The research by Stępniewska-Jarosz et al (2006) and Bełka and Mańka (2014) were partially conducted in the same area giving different results. In the later work (Bełka and Mańka, 2014) isolates AG4-HG2 were observed.…”

“…The difference may have resulted from possible bridging of some isolates belonging to certain AGs with isolates belonging to the other (Ogoshi, 1972) or from the fact that different techniques were used to assign isolates to certain AGs. While Stępniewska-Jarosz et al (2006) used classic techniques, with culture of isolates in vitro and subsequent observation of their growth under microscope, Bełka and Mańka (2014) based their study fully on molecular methods. As most of the research on Rhizoctonia not only in Poland, but worldwide has been conducted on agricultural plants, it is highly desirable to further screen forest nurseries for the pathogen and its anastomosis groups.…”