Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

Abstract

Glehnia littoralis is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte G. littoralis is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues of G. littoralis were illustrated with high throughput sequencing of the V3-V4 region of the bacterial 16S rRNA. The results resolved that the diversity and richness of endophytic bacteria were significantly higher in root than in leaf and stem. The operational taxonomic units (OTU) analysis demonstrated that the Actinobacteria and Proteobacteria were dominant in all the samples at the phylum level, and Pseudomonas, Bacillus, Rhizobium were the dominant genera. Our results unraveled that the bacterial communities differed among different tissues of G. littoralis. Endophytic bacterial communities in leaf and stem shared more similarity than that in the root. Furthermore, the difference of bacteria community and structure among different tissues were also detected by principal coordinate analysis. Taken altogether, we can conclude that the bacterial communities of different tissues are unique, which could facilitate understanding the diversity of endophytic bacteria in G. littoralis.

The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles

“…At present, research on the community structure of endophytic bacteria in plants mainly focuses on higher terrestrial plants, and much work has been done on isolating, identifying, and describing the physiological and biochemical characteristics of endophytic bacteria, as well as uncovering their interactions with hosts (Afzal et al, 2019;Ling et al, 2022;Yarte et al, 2022). In recent years, high-throughput sequencing technology has also been widely used to obtain a large amount of information on the endophytic bacterial community in terrestrial plants, includingthe crops Oryza sativa (Liu Y. et al, 2019), Zea mays and the medicinal plants Panax notoginseng (Zhang C. et al, 2020) and Gastrodia elata f. glauca (Zheng et al, 2022), as well as some aquatic plants, such as Lemnaceae, Pontederia crassipes, Pistia (Pramanic et al, 2023), and Glehnia littoralis (Huo et al, 2020). There have also been some studies on isolating, identifying, and describing the physiological activity and function of algal endophytic bacteria (Song et al, 2015;Zhang, 2017;Feng et al, 2020;Amri et al, 2023) based on culture methods.…”