Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field

Abstract

Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and 1H and 13C liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta.

A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H2O2 in Human Embryonic Kidney (HEK) 293T Cells

“…The FT-IR spectrum was recorded by using an IR Prestige-21 Fourier-transform infrared (FT-IR) spectrometer (Shimadzu, Japan) in the range of 4000–400 cm −1 . Briefly, 2 mg of the dried NMCP-2 was ground with 200 mg of KBr powder and pressed into 1-mm thick pellets for the analysis [36].…”

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

“…Compositions of other Cordyceps species were generally similar to those of C. sinensis [ 22 , 23 , 24 , 25 ], although some other monosaccharides, such as Rha, Xyl, Fru and Ara, were present in C. cicadae and C. kyushuensis [ 26 , 27 ]. In Morchella esculenta , another well-known ascomycete, the PSs contain four or more monosaccharides [ 28 , 29 , 30 ]. Zhao et al isolated 52 PSs from fermentation systems and fruiting bodies of Tuber species, and found that they were all composed mainly of Man, Glc, and Gal, suggesting that Tuber PSs have identical chemical compositions [ 31 ].…”

Secondary Metabolites and Antiradical Activity of Liquid Fermentation of Morchella sp. Isolated from Southwest China

“…These edible mushrooms appreciated worldwide have been used in traditional medicine for centuries, due to their health-related benefits. In recent research, the anti-oxidative, anti-inflammatory bioactivities, immunostimulatory, anti-tumor and antimicrobial properties were reported [1,2,3]. It was considered that morel’s health benefits were attributed mainly to sugar (polysaccharides) and to various constituents such as compounds, amino acids, important vitamin, fatty, organic acid and mineral profile [2,4].…”