Characteristic Variations in Lasiodiplodia theobromae; Pathogen of inflorescence Dieback of Cashew in Growing Ecologies of Nigeria

Adeniyi, Dele Omoyele; Olufolaji, D. B.; Joseph, Abiodun

doi:10.9734/arrb/2016/18047

Cited by 6 publications

(8 citation statements)

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“…Alves et al (2008) also reported presence of paraphyses within the conidiomata and the conidia initially hyaline and aseptate but with time a formation of septum and dark brown and melanin deposition gave conidia a striated appearance. Adeniyi et al (2016) reported significant variations observed in characters of L. theobromae isolates from dieback diseases of cashew and the similarity was observed in this study on conidia size (31.3 -42.9 × 15.6 -19.5 µm) of CDA1416 and conidia size of L. gonubiensis reported by Pavlic et al (2004) except for the multi-septate conidia nature of L. gonubiensis. However, conidia sizes of isolates in this study were somewhat larger than those reported by Burgess et al (2006) and Alves et al (2008) for L. theobromae.…”

Section: Discussionsupporting

confidence: 87%

“…A collection of isolates previously identified as L. theobromae, pathogen of inflorescence dieback disease of cashew in Nigeria (Adeniyi et al, 2011) was studied (Table 1), to investigate its mycelia and morphological characters according to the methods of Adeniyi et al (2016). Such characters include mycelia growth rate, colour pigmentation, pycnidial production and conidial dimension of the isolates.…”

Section: Methodsmentioning

confidence: 99%

“…This disease is a major focus of recent cashew pathology research in Nigeria. In-view of the wide spread of this pathogen, the large number of hosts and its character variability (Adeniyi et al, 2016), it is possible that L. theobromae on cashew composed of a number of traits considering diversity in their morphological characters expression. The purpose of this study was to determine variability within a collection of isolates previously identified as L. theobromae through analysis of their morphological quantitative parameters as part of measures to develop management strategies against the pathogen.…”

Section: Status Of Disease In Nigeriamentioning

confidence: 99%

See 2 more Smart Citations

Morphological Character Analysis and Signal Cryptic Speciation in Lasiodiplodia theobromae on Cashew (Anacardium occidentale L.)

Adeniyi¹,

Adewale²,

Nduka³

et al. 2017

JAS

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The research is financed by self and part of regular research mandate for the development of management strategies for the inflorescence die-back disease of cashew in Nigeria. AbstractLasiodiplodia theobromae (Pat) Griffon & Maubl. is a pathogen causing inflorescence dieback disease of cashew in Nigeria and also a common pathogen with a wide host range in the tropics and sub-tropics. The character variations in this pathogen necessitate better understanding of it towards development of management strategies. Isolates identified as L. theobromae were cultured from inflorescence dieback disease of cashew across growing ecologies of Nigeria and studied base on morphological characters. Variability in mycelial texture and colour, conidia and septa sizes and pycnidia production were recorded in this study. The Principal Component Analysis (PCA) and WARD clustering analysis identified four well-supported traits within the isolate group. Isolates within each cluster was: 2, 2, 4 and 1 respectively and isolate CDA1416 (Obollo-Afor) and CDA2924 (Idi-Ayunre) in cluster III were the most similar. Members within clusters I and II united at the semi-partial R-Square distance of 0.0294 and 0.0278 respectively. Isolate CDA2308 (Oro) was distinguished among others and signal a potential cryptic specie, differences in these isolates were supported by conidial morphology and textural variations. This understanding will form the bases for development of diseases management strategy against the pathogen.

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Status Of Disease In Nigeriamentioning

confidence: 99%

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Morphological Character Analysis and Signal Cryptic Speciation in Lasiodiplodia theobromae on Cashew (Anacardium occidentale L.)

Adeniyi¹,

Adewale²,

Nduka³

et al. 2017

JAS

View full text Add to dashboard Cite

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“…The developed fungal colonies were purified using hyphals or single spores. Micromorphological descriptions of mature conidiomata and conidia for 36 fungal cultures were carried out after 4 weeks of incubation using slides mounted in water following the methods described by Adeniyi et al (2016). Colony colors on the surface and reverse were assessed according to the color charts (Rayner 1970).…”

Section: Methodsmentioning

confidence: 99%

First Report of Lasiodiplodia theobromae Causing Needle Blight and Stem Canker Diseases on Araucaria heterophylla in Ethiopia

Darge

2017

Journal of Horticultural Research

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Canker and needle blight of Araucaria heterophylla (Norfolk Island pine) trees were observed during the surveys conducted in Addis Ababa and Adama cities (Ethiopia) from November to December 2016. The main objective of this study was to investigate fungal pathogens that cause diseases on Araucaria heterophylla trees. Six localities with Araucaria heterophylla plantings were purposively surveyed for disease symptoms. Samples from symptomatic parts of trees were collected, surface sterilized, cultured on PDA and morphologically identified for genus and species. A total of 36 isolates of fungi were identified. Based on macro- and microscopic morphological features of the colonies, the fungal isolates were found to be the genus Diplodia and species Lasiodiplodia theobromae (Pat.) Griffon & Maubl (syn. Botryodiplodia theobromae), the anamorph of Botryosphaeria rhodina (Berk. & M.A. Curtis) Arx. The pathogenicity test showed that the isolates of Lasiodiplodia theobromae, caused stem canker and needle blight on Araucaria heterophylla. This finding is important in the study of management options for future prevention and control of diseases in the country.

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“…Salamiah et al (2008) dan Sinaga et al (2009) melaporkan bahwa beberapa kebun di sentra produksi jeruk di Indonesia mengalami busuk batang gumosis dan mati meranggas yang disebabkan oleh Botryodiplodia theobromae sehingga menyebabkan penurunan produktivitas jeruk hingga 53.9%. Patogen ini memiliki kisaran inang lebih dari 280 spesies tanaman dan menyebabkan gejala nekrosis batang, diikuti dengan gumosis, busuk batang, mati pucuk, dan mati meranggas (Adeniyi et al 2016).…”

Section: Pendahuluanunclassified

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya

Khairani

Sinaga

Mutaqin

2017

J Fitopatol Indones

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ABSTRAKProduktivitas tanaman jeruk seringkali dibatasi oleh penyakit tanaman. Di Indonesia, penyakit busuk batang yang disebabkan oleh Botryodiplodia theobromae menjadi penyakit penting yang menyebabkan tanaman jeruk mati meranggas. Penelitian dilakukan untuk mempelajari mekanisme pengendalian B. theobromae oleh khamir, cendawan mikoriza arbuskular (CMA), bakteri simbiotik CMA dan kitosan. Uji in vitro dilakukan untuk mengevaluasi mekanisme antibiosis, hiperparasitisme, produksi senyawa volatil, dan produksi enzim kitinase. Percobaan dilanjutkan dengan pengujian in planta terhadap khamir, CMA, bakteri simbiotik CMA terpilih, dan kitosan baik secara tunggal maupun kombinasi. Percobaan disusun dalam rancangan acak lengkap dengan 3 ulangan. Peubah yang diamati ialah periode laten, insidensi dan keparahan penyakit, laju infeksi, area under disease progress curve (AUDPC), tingkat asosiasi CMA, dan kandungan fenol total. Hasil uji in vitro menunjukkan bahwa khamir mempunyai aktivitas hiperparasitisme pada B. theobromae dengan afinitas 26 sel per hifa, memproduksi senyawa volatil dengan tingkat hambatan relatif (THR) 29.1%, dan memproduksi enzim kitinase. Isolat bakteri simbiotik CMA terbaik menunjukkan mekanisme antibiosis dengan THR 42.9% dan memproduksi senyawa volatil dengan THR 26.7%. Isolat ini memiliki homologi 98% dengan Bacillus subtilis asal Vietnam. Perlakuan khamir + CMA + kitosan memperlambat periode laten dan menekan insidensi penyakit. Perlakuan tunggal CMA dan kombinasi khamir + CMA menekan keparahan penyakit, laju infeksi, dan nilai AUDPC. Aplikasi khamir + CMA menunjukkan tingkat asosiasi CMA dan peningkatan kandungan fenol total paling tinggi. Berdasarkan hasil penelitian ini, perlakuan tunggal CMA dan khamir + CMA direkomendasikan dalam pengendalian penyakit busuk batang jeruk.Kata kunci: antibiosis, Botryodiplodia theobromae, hiperparasitisme, kitinase ABSTRACT Plant diseases become an important constraint on citrus production. Stem rot disease caused by Botryodiplodia theobromae is a major disease on citrus in Indonesia. This study was aimed to evaluate the mechanism of yeast, arbuscular mychorrhiza fungi (AMF), symbiotic bacteria of AMF and chitosan in controlling stem rot disease. In vitro study was performed to evaluate the mechanism of antibiosis, hyperparasitism, production of volatile compounds, and production of chitinase enzyme. The experiment was continued by in planta assays using yeast, AMF, symbiotic bacteria of AMF, and chitosan either singly or in combination. The experiments were performed using completely randomized

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Characteristic Variations in Lasiodiplodia theobromae; Pathogen of inflorescence Dieback of Cashew in Growing Ecologies of Nigeria

Cited by 6 publications

References 0 publications

Morphological Character Analysis and Signal Cryptic Speciation in Lasiodiplodia theobromae on Cashew (Anacardium occidentale L.)

Morphological Character Analysis and Signal Cryptic Speciation in Lasiodiplodia theobromae on Cashew (Anacardium occidentale L.)

First Report of Lasiodiplodia theobromae Causing Needle Blight and Stem Canker Diseases on Araucaria heterophylla in Ethiopia

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya

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