Characteristic Spectral Features of the Polarized Fluorescence of Human Breast Cancer in the Wavelet Domain

Abstract

Wavelet transform of polarized fluorescence spectra of human breast tissues is found to localize spectral features that can reliably differentiate normal and malignant tissue types. The intensity differences of parallel and perpendicularly polarized fluorescence spectra are subjected to investigation, since they are relatively free of diffusive background. A number of parameters, capturing spectral variations and subtle changes in the diseased tissues in the visible wavelength regime, are clearly identifiable in the wavelet domain. These manifest both in the average low-pass and high frequency high-pass wavelet coefficients.

Detecting cervical cancer progression through extracted intrinsic fluorescence and principal component analysis

“…There is evidence of porphyrin fluorescence in breast tumors from recent studies. [59][60][61] Thus, in spite of a large standard deviation as seen in Fig. 2, distinct differences are still observed in the corresponding correlation maps of intrinsic fluorescence.…”

Intrinsic fluorescence for cervical precancer detection using polarized light based in-house fabricated portable device

“…Fluorescence spectroscopy is one of the relatively sensitive methods to probe subtle biochemical changes and this has been used for in vitro and in vivo studies over the past three decades. [8][9][10][11][12][13][14][15][16][17][18] Since the fluorescence from tissue is significantly modified by absorption and scattering effects at both excitation and emission wavelengths, important diagnostic information of biochemical changes with disease progression are hidden. It is thus necessary to extract intrinsic fluorescence.…”

Mueller-matrix of laser-induced autofluorescence of polycrystalline films of dried peritoneal fluid in diagnostics of endometriosis

“…The diagnostic application of the method of spectral fluorometry of whole blood, including serum and plasma, in oncology and other areas of medicine is based on the effect of increasing fluorescence intensity of the porphyrins in the "red" range of the spectrum. 27,28 However, in the modern literature there are a comparatively small number of publications 20,[29][30][31] on polarization manifestations of fluorescence effects in biological tissues and fluids. Thus, the topical task of complex unification of diagnostic potentiality of both the techniques of laser polarimetry and laser autofluorescence proves to be important.…”