Characteristic's of trophoblast cells migrating from first trimester chorionic villus explants and propagated in culture

Irving, J.A.; Lysiak, Jeffrey J.; Graham, Charles H.; Hearn, Stephen; Han, V. K. M.; Lala, Peeyush K.

doi:10.1016/0143-4004(95)90100-0

Cited by 183 publications

(92 citation statements)

References 43 publications

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“…These cells stain immunohistochemically for cytokeratin and the ␣-and ␤-chains of human chorionic gonadotropin, but not vimentin (46 -48). HTR 8 cells were derived from outgrowths of first-trimester chorionic villi (49), express cytokeratin and HLA framework antigens (50), and produce ␤-chain of human chorionic gonadotropin (49,51). Human aortic smooth muscle cells, which express both LRP/␣ 2 MR and the VLDL receptor, were isolated and cultured as described previously (52).…”

Section: Methodsmentioning

confidence: 99%

The Low Density Lipoprotein Receptor-related Protein/α2-Macroglobulin Receptor Regulates Cell Surface Plasminogen Activator Activity on Human Trophoblast Cells

Zhang¹,

Sakthivel²,

Kniss³

et al. 1998

Journal of Biological Chemistry

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The low density lipoprotein receptor-related protein/ ␣ 2 -macroglobulin receptor (LRP/␣ 2 MR) mediates the internalization of numerous ligands, including prourokinase (pro-UK) and complexes between two-chain urokinase (tc-u-PA) and plasminogen activator inhibitor type-1 (PAI-1). It has been suggested that through its ability to internalize these ligands, LRP/␣ 2 MR may regulate the expression of plasminogen activator activity on cell surfaces; this hypothesis, however, has not been experimentally confirmed. To address this issue, we assessed the ability of LRP/␣ 2 MR to regulate plasminogen activator activity on human trophoblast cells, which express both LRP/␣ 2 MR and the urokinase receptor (uPAR). Trophoblasts internalized and degraded exogenous 125 I-pro-UK (primarily following its conversion to tc-u-PA and incorporation into tc-u-PA⅐PAI complexes) in an LRP/␣ 2 MR-dependent manner, which was inhibited by the LRP/␣ 2 MR receptor-associated protein. Receptor-associated protein also caused a ϳ50% reduction in cell surface plasminogen activator activity and delayed the regeneration of unoccupied uPAR by cells on which uPAR were initially saturated with pro-UK. Identical effects were caused by anti-LRP/␣ 2 MR antibodies. These results demonstrate that LRP/␣ 2 MR promotes the expression of cell surface plasminogen activator activity on trophoblasts by facilitating the clearance of tc-u-PA⅐PAI complexes and regeneration of unoccupied cell surface uPAR.

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Section: Methodsmentioning

confidence: 99%

The Low Density Lipoprotein Receptor-related Protein/α2-Macroglobulin Receptor Regulates Cell Surface Plasminogen Activator Activity on Human Trophoblast Cells

Zhang¹,

Sakthivel²,

Kniss³

et al. 1998

Journal of Biological Chemistry

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“…EVTs may also be a target for P. gingivalismediated DDP. HTR8/SvNeo cells are human EVTs [90,91] that were originally isolated from first trimester placental tissue and immortalized with the pSV3neo plasmid [92]. HTR8/SvNeo cells retain the EVT phenotype (i.e.…”

Section: P Gingivalis As a Vascular Pathogen Of The Placental Bedmentioning

confidence: 99%

Porphyromonas gingivalisand adverse pregnancy outcome

Reyes

Phillips

Wolfe

et al. 2017

Journal of Oral Microbiology

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Porphyromonas gingivalis is a Gram-negative, anaerobic bacterium considered to be an important pathogen of periodontal disease that is also implicated in adverse pregnancy outcome (APO). Until recently, our understanding of the role of P. gingivalis in APO has been limited and sometimes contradictory. The purpose of this review is to provide an overview of past and current research on P. gingivalis that addresses some of the controversies concerning the role of this organism in the pathogenesis of APO.ARTICLE HISTORY

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“…These cells were acquired from human explant cultures obtained from the first trimester placenta and immortalized through transfection using a cDNA construct encoding the SV40 large T antigen 22 . These non-tumorigenic and metastatic cells are highly invasive in vitro and exhibit various markers of extravillous trophoblasts in situ 21,22 . HTR8/ SVneo cells were seeded (1 mL/well, 5 3 10 5 /mL) onto a 6-well culture plate (BD Biosciences, Franklin Lakes, NJ, USA) and incubated for 4 hours to facilitate attachment.…”

Section: Cell Isolation and Sortingmentioning

confidence: 99%

Human trophoblast cells induced MDSCs from peripheral blood CD14+ myelomonocytic cells via elevated levels of CCL2

Zhang

Sun

et al. 2015

Cell Mol Immunol

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Successful human pregnancy requires the maternal immune system to recognize and tolerate the semi-allogeneic fetus. Myeloid-derived suppressor cells (MDSCs), which are capable of inhibiting T-cell responses, are highly increased in the early stages of pregnancy. Although recent reports indicate a role for MDSCs in fetal-maternal tolerance, little is known about the expansion of MDSCs during pregnancy. In the present study, we demonstrated that the trophoblast cell line HTR8/SVneo could instruct peripheral CD14 1 myelomonocytic cells toward a novel subpopulation of MDSCs, denoted as CD14 1 HLA-DR 2/low cells, with suppressive activity and increased expression of IDO1, ARG-1, and COX2. After interaction with HTR8/SVneo cells, CD14 1 myelomonocytic cells secrete high levels of CCL2, promoting the expression of signal transducer and activator of transcription 3. We utilized a neutralizing monoclonal antibody to reveal the prominent role of CCL2 in the induction of CD14 1 HLA-DR 2/low MDSCs. In combination, the results of the present study support a novel role for the cross-talk between the trophoblast cell line HTR8/SVneo and maternal CD14 1 myelomonocytic cells in initiating MDSCs induction, prompting a tolerogenic immune response to ensure a successful pregnancy. Cellular & Molecular Immunology

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Characteristic's of trophoblast cells migrating from first trimester chorionic villus explants and propagated in culture

Cited by 183 publications

References 43 publications

The Low Density Lipoprotein Receptor-related Protein/α2-Macroglobulin Receptor Regulates Cell Surface Plasminogen Activator Activity on Human Trophoblast Cells

The Low Density Lipoprotein Receptor-related Protein/α2-Macroglobulin Receptor Regulates Cell Surface Plasminogen Activator Activity on Human Trophoblast Cells

Porphyromonas gingivalisand adverse pregnancy outcome

Human trophoblast cells induced MDSCs from peripheral blood CD14+ myelomonocytic cells via elevated levels of CCL2

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